A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was evaluate influence wood-based cellulose nanofibrils (CNF) bioactive glass (BaG) on rheological properties gelatin–alginate bioinks initial responses bone cells embedded these inks. CNF modulated flow behavior hydrogels, thus improving their printability. Chemical characterization by SEM-EDX ion release analysis confirmed reactivity BaG...

Degradable and electrically conductive polysaccharide hydrogels (DECPHs) have been synthesized by functionalizing with aniline oligomers. DECPHs based on chitosan (CS), tetramer (AT), glutaraldehyde were obtained a facile one-pot reaction using the amine group of CS AT under mild conditions, which avoids multistep reactions tedious purification involved in synthesis degradable our previous work. Interestingly, these possess good film-forming properties, electrical conductivity, pH-sensitive...

Degradable electrically conducting hydrogels (DECHs), which combine the unique properties of degradable polymers and hydrogels, were synthesized by introducing biodegradable segments into conductive hydrogels. These DECHs obtained joining together photopolymerized macromer acrylated poly(d,l-lactide)−poly(ethylene glycol)−poly(d,l-lactide) (AC-PLA-PEG-PLA-AC), glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) network aniline tetramer (AT) coupling reaction between AT GMA....

A low dose of 1μg rhBMP-2 was immobilised by four different functionalising techniques on recently developed poly(l-lactide)-co-(ε-caprolactone) [(poly(LLA-co-CL)] scaffolds. It either (i) physisorbed unmodified scaffolds [PHY], (ii) onto modified with nanodiamond particles [nDP-PHY], (iii) covalently linked nDPs that were used to modify the [nDP-COV] or (iv) encapsulated in microspheres distributed [MICS]. Release kinetics BMP-2 from quantified using targeted mass spectrometry for up...

The dynamic interplay between cells and their native extracellular matrix (ECM) influences cellular behavior, imposing a challenge in biomaterial design. Dynamic covalent hydrogels are viscoelastic show self-healing ability, making them potential scaffold for recapitulating ECM properties. We aimed to implement kinetically thermodynamically distinct crosslinkers prepare explore the arising properties effects on behavior. To do so, aldehyde-substituted hyaluronic acid (HA) was synthesized...

In this article the effects of number molecular branches (chain ends) and stereochemistry poly(lactide)s (PLAs) on enzymatic degradation alkaline hydrolysis are studied. Various linear branched PLAs were synthesized using lipase PS (Pseudomonas fluorescens)-catalyzed ring-opening polymerization (ROP) lactide monomers having different stereochemistries (l-lactide, d-lactide, d,l-lactide). Five alcohols used as initiators for ROP, monomer-to-initiator molar feed ratio was varied from 10 to 100...

Two-, four-, and six-armed branched copolymers with electroactive biodegradable properties were synthesized by coupling reactions between poly(l-lactides) (PLLAs) different architecture carboxyl-capped aniline trimer (CCAT). The oligomer CCAT was prepared from amino-capped succinic anhydride. FT-IR, NMR, SEC analyses confirmed the structure of copolymers. UV−vis spectra cyclic voltammetry copolymer solution showed good properties, similar to those polyaniline. water contact angle PLLAs...

We present macromolecular architecture design as a useful tool to enhance the conductivity of degradable polymers. Linear and hyperbranched copolymers with electrical biodegradability were synthesized by an "A2 + Bn (n = 2, 3, 4)" strategy using carboxyl-capped aniline pentamer (CCAP) branched poly(ε-caprolactone)s (PCLs) coupling reactions. A more hydrophilic surface lower crystallinity doped emeraldine state (EMAP) copolymer was achieved compared PCLs, TGA results demonstrated that CCAP...

Vinyl polymers, e.g. polyvinyl acetate and alcohol, have properties which suit tissue engineering in many perspectives. A copolymer of 2-methylene-1,3-dioxepane (MDO) with vinyl (VAc) should combine the desirable polymers degradability aliphatic polyesters. Our results detailed kinetics information show that it is possible to vary amount MDO widely copolymer. Between 1 70% was incorporated into polymer when copolymerized VAc at 60 °C using 2,2-azobisisobutyronitrile (AIBN) as an initiator....

Abstract Introduction Rapid establishment of functional blood vessels is a prerequisite for successful tissue engineering. During vascular development, endothelial cells (ECs) and perivascular assemble into complex regulating proliferation ECs, vessel diameter production extracellular matrix proteins. The aim this study was to evaluate the ability mesenchymal stem (MSCs) establish an endothelial-perivascular in tissue-engineered constructs comprising ECs MSCs. Methods Primary human MSCs were...

Abstract Functionalizing aliphatic polyester hydrogels with an aniline oligomer is a means of achieving electrically conductive and degradable hydrogels. To lower the content while maintaining high conductivity to overcome acidic degradation product from polylactide reported in our previous work, series electroactive based on polycaprolactone (PCL) carboxyl‐capped pentamer (CCAP) were synthesized by simple coupling reaction at room temperature. The was carried out between hydroxyl groups...

The understanding of cell-material interactions is important for creating personalized implants tissue engineering. This has resulted in an interest developing polymers with functional groups the possibility controlling macromolecular surface. We have a one-pot reaction synthesized series amorphous and degradable polyester-based copolymers active by copolymerization 2-methylene-1,3-dioxepane glycidyl methacrylate. properties final were varied varying feed ratios monomers, it was seen that...

Covalent surface functionalization is presented as a versatile tool to increase the hydrophilicity and introduce electroactivity of polyester films. Acrylic acid maleic anhydride were photografted onto polylactide (PLA) with "grafting from" method wettability, subsequent coupling conductive aniline oligomer was used PLA surface. The photopolymerization acrylic tetramer (AT) characterized by FT-IR, UV, TGA. morphology before after modification examined scanning electron microscopy (SEM)...

Significant evidence has indicated that poly(L-lactide)-co-(ɛ-caprolactone) [(poly(LLA-co-CL)] scaffolds could be one of the suitable candidates for bone tissue engineering. Oxygen-terminated nanodiamond particles (n-DP) were combined with poly(LLA-co-CL) and revealed to positive cell growth. In this study, we evaluated influence modified by n-DP on attachment, proliferation, differentiation marrow stromal cells (BMSCs) in vitro, formation using a sheep calvarial defect model. BMSCs seeded...

Degradable porous polymeric structures are attractive candidates for biological tissue scaffolds, and adequate mechanical, transport, chemical properties determine their functionality. Aside from the of polymer-based materials, scaffold's meso-structure controls its elasticity at organ length-scale. This study investigated effect on scaffolds' mechanical transport using finite element analysis (FEA) computational fluid dynamics (CFD). A number poly (ε-caprolactone) (PCL) - based scaffolds...

Various 3D printing techniques currently use degradable polymers such as aliphatic polyesters to create well-defined scaffolds. Even though are influenced by the process, and this subsequently affects mechanical properties degradation profile, of polymer during process is not often considered. Degradable scaffolds today printed cell-material interactions evaluated without considering fact that change while scaffold. Our methodology herein was vary parameters temperature, pressure, speed...

Abstract Adipose-derived stem cells (ASC) have been used as an alternative to bone marrow mesenchymal (BMSC) for tissue engineering. However, the efficacy of ASC in regeneration comparison with BMSC remains debatable, since inconsistent results reported. Comparing obtained from different individuals might contribute this inconsistency results. Therefore, study aimed compare regenerative capacity donor-matched human and seeded onto poly( l -lactide-co-ε-caprolactone) scaffolds using calvarial...

A simple and effective method of fabricating scaffolds with open pore structures was successfully used on several copolymers. The method, which is straightforward fast, developed to overcome problems such as low interconnectivity achieve thick three-dimensional scaffolds. Copolymers are particular interest because it possible tune their mechanical degradable properties, in this work, copolymers l-lactide (LLA) trimethylene carbonate (TMC) were synthesized through ring-opening polymerization....

We present a universal strategy for the facile synthesis of degradable and electroactive block copolymers organogels (DEBCGs) based on aniline oligomers polyesters in two-step approach, here exemplified by preparation series DECBCGs tetramer (AT) poly(ε-caprolactone) (PCL). Polyesters with an dimer (AD) segment were first obtained controlled ring-opening polymerization (ROP) ε-caprolactone initiated amine group AD or without 2,2-bis(ε-caprolactone-4-yl) propane (BCP). The postpolymerization...