A5007372145- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Periodontal Regeneration and Treatments
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- Tissue Engineering and Regenerative Medicine
- Retinoids in leukemia and cellular processes
- Chronic Myeloid Leukemia Treatments
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Tryptophan and brain disorders
- CAR-T cell therapy research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Osteoarthritis Treatment and Mechanisms
- Bone fractures and treatments
- Erythrocyte Function and Pathophysiology
- Acute Myeloid Leukemia Research
- Bone Tissue Engineering Materials
- Acute Lymphoblastic Leukemia research
- Immune cells in cancer
German Red Cross
2014-2025
University Hospital Ulm
2014-2023
Universität Ulm
2012-2022
DRK-Blutspendedienst Baden-Württemberg - Hessen
2008-2021
Immungenetics (Germany)
2006-2019
Helmholtz-Institute Ulm
2009
Institute for Transfusion Medicine
2007-2008
University of Tübingen
2005
University of Victoria
2005
Freie Universität Berlin
2000-2003
Proper activation of macrophages (Mφ) in the inflammatory phase acute wound healing is essential for physiological tissue repair. However, there a strong indication that robust Mφ responses may be causal fibrotic response always accompanying adult healing. Using complementary approach vitro and vivo studies, we here addressed question whether mesenchymal stem cells (MSCs)-due to their anti-inflammatory properties-would control fibrosis murine model full-thickness skin wounds. We have shown...
Autologous grafting, despite some disadvantages, is still considered the gold standard for reconstruction of maxillofacial bone defects. The aim this study was to evaluate regeneration using marrow-derived mesenchymal stromal cells (MSCs) in a clinical trial, less invasive approach than autologous grafting. This comprehensive trial included subjects with severe mandibular ridge resorption. 11 aged 52–79 years Bone marrow were aspirated from posterior iliac crest and plastic adherent expanded...
BACKGROUND: Cytomegalovirus (CMV) disease constitutes a serious complication after allogeneic stem cell transplantation. For the clearance of CMV, CD8+ T cells are pivotal. STUDY DESIGN AND METHODS: Here, novel streptamer technology was used at good manufacturing practice (GMP) level for adoptive transfer CMV‐specific into acute leukemia patients with recurrent high CMV antigenemia RESULTS: After single transfusion, frequency CD8+CD45RA+CCR7– effector increased dramatically from 0.0% to...
Background Mesenchymal stromal cells (MSC) have gained importance in tissue repair, engineering and immunosupressive therapy during the last years. Due to limited availability of MSC bone marrow, ex vivo amplification prior clinical application is requisite obtain therapeutic applicable cell doses. Translation preclinical into clinical-grade large-scale expansion necessitates precise definition standardization all procedural parameters including seeding density, culture medium cultivation...
Background Recently, we demonstrated immunological and clinical responses to a RHAMM-R3 peptide vaccine in patients with acute myeloid leukemia, myelodysplastic syndrome multiple myeloma. To improve the outcome of vaccine, second cohort was vaccinated higher dose 1,000 μg peptide.Design Methods Nine received four vaccinations subcutaneously at biweekly interval. Immunomonitoring cytotoxic CD8+ as well regulatory CD4+ T cells performed by flow cytometry enzyme-linked immunospot (ELISpot)...
The estimated frequency of MSCs in BM is about 0.001–0.01% total nucleated cells. Most commonly, one applied therapeutic cell dose 1–5 million MSCs/kg body weight, necessitating a reliable, fast, and safe expansion system. limited availability demands for an extensive ex vivo amplification step to accumulate sufficient numbers. Human platelet lysate (PL) has proven be feasible alternative animal-derived serum as supplement MSC cultivation. We have investigated the functionally closed...
ORTHO-1 is a European, multicentric, first in human clinical trial to prove safety and feasibility after surgical implantation of commercially available biphasic calcium phosphate bioceramic granules associated during surgery with autologous mesenchymal stromal cells expanded from bone marrow (BM-hMSC) under good manufacturing practices, patients long pseudarthrosis.Twenty-eight femur, tibia or humerus diaphyseal metaphyso-diaphyseal non-unions were recruited surgically treated France,...
Repair of large bone defects remains a significant clinical challenge. Bone marrow stromal cells (BMSCs), subset which is known as marrow-derived mesenchymal stem cells, show therapeutic potential for regeneration. However, their isolation, expansion and implantation will need to be conducted under good manufacturing practices (GMP) at separate locations. An investigation mimics this scenario where shall regenerated required before trials can initiated. Seven batches 100 million human...
SUMMARY: Research on expanded human stem cells has become an increasing field of interest during the last decade. The in adult cells, especially mesenchymal and stromal hematology regenerative medicine is also based simplicity isolation ex vivo expansion these cells. These processes require adequate quality control source product. In this review, we summarize various different attempts to characterize surface protein expression by flow cytometry define multipotent subpopulations for...
The poor healing potential of tendons is still a clinical problem, and the use Platelet Rich Plasma (PRP) was hypothesized to stimulate healing. As efficacy PRPs remains unproven, platelet lysate (PL) could be an alternative with its main advantages storage characterization before use. Five different blood products were prepared from 16 male donors: human serum, two (Arthrex, (PRP-ACP); RegenLab (PRP-BCT)), concentrate (apheresis, PC), PL (freezing-thawing destruction PC). Additionally, ten...
Advanced therapy medicinal products (ATMP) frequently lack of clinical data on efficacy to substantiate a future use. This study aims evaluate the heal long bone delayed unions and non-unions, as secondary objective EudraCT 2011-005441-13 trial, through radiological consolidation at 3, 6 12 months follow-up, with subgroup analysis affected bone, gender, tobacco use, time since original fracture.Twenty-eight patients were recruited surgically treated autologous marrow derived mesenchymal...
Highly sensitive and specific platforms for the detection of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies are becoming increasingly important evaluating potential SARS-CoV-2 convalescent plasma donors, studying spread infections, identifying individuals with seroconversion. This study provides a comparative validation 4 anti-SARS-CoV-2 platforms. A unique feature is use representative cohort patients disease 2019 mild to moderate course. All showed significant...
Mesenchymal stromal cells (MSCs) are promising therapeutic candidates in a variety of diseases due to having immunomodulatory and pro-regenerative properties. In recent years, MSC-derived small extracellular vesicles (sEVs) have attracted increasing interest as possible alternative conventional cell therapy. However, translational processes sEVs for clinical applications still impeded by inconsistencies regarding isolation procedures culture conditions. We systematically compared different...
Abstract Necrosis is a characteristic feature of advanced solid tumors. Released necrotic factors, also referred to as damage associated molecular patterns (DAMPs), are known critically impact the tumor microenvironment by enhancing angiogenesis or influencing immune response. We have recently shown that DAMPs can act chemoattractants and activators granulocytes. demonstrate material from both normal cells promotes proliferation trafficking human mesenchymal stem (MSCs). characterize protein...
The immune reconstitution after allogeneic hematopoietic stem cell transplantation comprises thymus-dependent and thymus-independent pathways. We wanted to improve the understanding of this complex process using two different measurements at definite checkpoints T-cell neogenesis. therefore assessed pathway by combining single joint receptor excision circles (sjTREC) β (βTREC) in an improved quantitative light-cycler hybridization polymerase chain reaction assay. In a subgroup patients, we...
Abstract The novel selective BCR‐ABL Breakpoint cluster region – Abelson murine leukemia viral oncogene homolog 1 (BCR‐AML) inhibitor nilotinib (AMN107) is a tyrosine kinase that more potent against leukaemia cells in vitro than imatinib. As might be used the context of allogeneic stem cell transplantation where CD8 + T lymphocytes play pivotal role graft‐versus‐leukaemia (GVL) effect, we investigated effects on this lymphocyte subpopulation. Nilotinib inhibits phytohemagglutinin...