A5041909354- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- Heat shock proteins research
- Sarcoma Diagnosis and Treatment
- Mechanisms of cancer metastasis
- Bone health and treatments
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Cancer Research and Treatments
- Glutathione Transferases and Polymorphisms
- Cytokine Signaling Pathways and Interactions
- Bone Tumor Diagnosis and Treatments
- Sirtuins and Resveratrol in Medicine
- Orthopedic Infections and Treatments
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- Orthopaedic implants and arthroplasty
- Kruppel-like factors research
- Rheumatoid Arthritis Research and Therapies
- Bone fractures and treatments
- Cancer-related molecular mechanisms research
- Psoriasis: Treatment and Pathogenesis
- Bone Tissue Engineering Materials
Nantes Université
2016-2025
Inserm
2016-2025
Centre National de la Recherche Scientifique
2022-2024
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2022-2024
Université d'Angers
2023
École Pratique des Hautes Études
2021
La Ligue Contre le Cancer
2013-2016
Université Nantes Angers Le Mans
2015
Hôtel-Dieu de Paris
2014
IMT Atlantique
2013
Interleukin (IL)-36α, IL-36β and IL-36γ are expressed highly in skin involved the pathogenesis of psoriasis, while antagonists IL-36Ra or IL-38, another potential IL-36 inhibitor, limit uncontrolled inflammation. The expression role cytokines rheumatoid arthritis (RA) Crohn's disease (CD) is currently debated. Here, we observed that during imiquimod-induced mouse inflammation human IL-36α, γ IL-36Ra, but not IL-38 mRNA, was induced correlated with IL-1β T helper type 17 (Th17) (IL-17A,...
Interleukin‐34 (IL‐34) was recently characterized as the M‐CSF “twin” cytokine, regulating proliferation/differentiation/survival of myeloid cells. The implication in oncology initially suspected by reduced metastatic dissemination knock‐out mice, due to angiogenesis impairment. Based on this observation, our work studied involvement IL‐34 pathogenesis osteosarcoma. vivo effects were assessed tissue vasculature and macrophage infiltration a murine preclinical model based paratibial...
Objectives Interleukin (IL) 34 is a new cytokine implicated in macrophage differentiation and osteoclastogenesis. This study assessed IL-34 expression the tissue of patients with rheumatoid arthritis (RA). Methods Immunohistochemistry was performed synovial biopsies from RA (n=20), osteoarthritis (n=3) or other inflammatory (n=4). detected fluid by ELISA its messenger RNA studied quantitative PCR fibroblasts after stimulation tumour necrosis factor α (TNFα) IL-1β. Wild-type, jnk1 −/− –jnk2...
Human adipose tissue-derived stem cells (hATSC) have been contemplated as reparative for cartilage engineering. Chondrogenic differentiation of hATSC can be induced by an enriched culture medium and a three-dimensional environment. Given that bone is vascularized not, oxygen tension has suggested regulatory factor osteochondrogenic differentiation. Our work aimed at determining whether hypoxia affects the potential hATSC. were cultured in chondrogenic or osteogenic 28 days, pellets...
Dental pulp is a dynamic tissue able to resist external irritation during tooth decay by using immunocompetent cells involved in innate and adaptive responses. To better understand the immune response of toward gram-negative bacteria, we analyzed biological mediators rat incisor experimentally inflamed either lipopolysaccharide (LPS) or saline solution (phosphate-buffered [PBS]). Untreated teeth were used as control. Expression pro- anti-inflammatory cytokines, chemokine ligands, growth...
Repair of large bone defects remains a significant clinical challenge. Bone marrow stromal cells (BMSCs), subset which is known as marrow-derived mesenchymal stem cells, show therapeutic potential for regeneration. However, their isolation, expansion and implantation will need to be conducted under good manufacturing practices (GMP) at separate locations. An investigation mimics this scenario where shall regenerated required before trials can initiated. Seven batches 100 million human...
Osteosarcoma is the main malignant primary bone tumor in children and adolescents for whom prognosis remains poor, especially when metastasis present at diagnosis. Because transforming growth factor-β (TGFβ) has been shown to promote many solid tumors, we investigated effect of natural TGFβ/Smad signaling inhibitor Smad7 TβRI SD-208 on osteosarcoma behavior.By using a mouse model induced by paratibial injection cells, assessed impact overexpression or growth, microenvironment, remodeling,...
Primary cancer cell dissemination is a key event during the metastatic cascade, but context-specific determinants of this process remain largely undefined. Multiple reports have suggested that p53 (TP53) family member p63 (TP63) plays an antimetastatic role through its minor epithelial isoform containing N-terminal transactivation domain (TAp63). However, and contribution major lacking domain, ΔNp63α, Here, we report distinct TAp63-independent mechanism by which ΔNp63α-expressing cells...
// Camille Jacques 1, 2 , François Lamoureux Marc Baud'huin 2, 3 Lidia Rodriguez Calleja Thibaut Quillard Jérôme Amiaud Franck Tirode 4 Françoise Rédini James E. Bradner 5, 6 Dominique Heymann Benjamin Ory 1 INSERM, UMR 957, Équipe Labellisée Ligue 2012, Nantes, France Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, Université Nantes Atlantique Universités, EA3822, University Hospital, Institut Curie, INSERM U830, Paris, 5 Department of Medical...
Oncostatin M (OSM) has been reported to be overexpressed in psoriasis skin lesions and exert proinflammatory effects vitro on human keratinocytes. Here, we report the role of OSM vivo a mouse model inflammation induced by intradermal injection murine OSM-encoding adenovirus (AdOSM) compare with that IL-6 injection. show potently regulates expression genes involved epidermal differentiation primary In vivo, AdOSM ears provoked robust thickening keratinocyte proliferation, while minimal effect...
Classically, particle-induced periprosthetic osteolysis at the implant–bone interface has explained aseptic loosening of joint replacement. This response is preceded by triggering both innate and acquired immune with subsequent activation osteoclasts, bone-resorbing cells. Although been considered a foreign body chronic inflammation mediated myelomonocytic-derived cells, current reports describe wide heterogeneous inflammatory cells infiltrating tissues. review aims to discuss role those...
Abstract Introduction Osteonecrosis of the femoral head (ONFH) is a degenerative disease progressing to (FH) collapse. Injection osteoprogenitor cells like bone marrow mesenchymal stromal (BMSCs) into FH appears be good therapeutic treatment. However, safety and efficacy BMSCs treat defect are main preclinical data required for clinical application. Efficacy lack risk cell transformation after amplification have been extensively described. The objectives this study were develop simple usable...
// Audrey Lamora 1, 2, 3, 4 , Mathilde Mullard 3 Jérôme Amiaud Régis Brion Dominique Heymann Françoise Redini Franck Verrecchia 1 INSERM, UMR 957, Equipe Labellisée Ligue contre le Cancer 2012, Nantes, France 2 Université de Laboratoire Physiopathologie la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, CHU Hôtel Dieu, Inserm Liliane Bettencourt School, Correspondence to: Verrecchia, e-mail: franck.verrecchia@inserm.fr Keywords: halofuginone, osteosarcoma, TGF-β, metastases,...
Background: Osteosarcoma is the most frequent form of malignant pediatric bone tumor. Despite current therapeutic arsenal, patient life-expectancy remains low if metastases are detected at time diagnosis, justifying research into better knowledge all stages osteosarcoma ontogenesis and identification new targets. Receptor Activator Nuclear factor κB (RANK)expression has been reported in cells, raising question Ligand (RANKL)/RANK signaling implications these tumor cells (intrinsic), addition...
Bone destruction is a hallmark of chronic inflammation, and bone-resorbing osteoclasts arising under such condition differ from steady-state ones. However, osteoclast diversity remains poorly explored. Here, we combined transcriptomic profiling, differentiation assays in vivo analysis mouse to decipher specific traits for inflammatory osteoclasts. We identified validated the pattern-recognition receptors (PRR) Tlr2, Dectin-1, Mincle, all involved yeast recognition as major regulators showed...
Rationale: During development, the contribution of IL34, a ligand macrophage colony stimulating factor receptor (MCSFR), has not been fully defined. Together with its twin cytokine MCSF, they display an essential role in differentiation and activation, including tissue specialized macrophages. The mechanism action each molecule involves phosphorylation MCSFR varying intensity kinetics. Furthermore, IL34 can interact other receptors cofactors, opening wide range modulations during...
Osteosarcoma is medically defined as a bone-forming tumor with associated bone-degrading activity. There lack of knowledge about the network that generates overproduction bone. We studied early stage osteosarcoma development mice enduring periosteum injection cells at proximal third tibia. On day 7 (D7), activate over-synthesis bone-like material inside medulla. This bone quickly (D13) followed by degradation. Samples were characterized microfocus small-angle X-ray scattering (SAXS),...
Osteosarcoma (OS) is the most common malignant bone tumor in children and teenagers. In many cases, such as poor response to treatment or presence of metastases at diagnosis, survival rate patients remains very low. Although literature, more studies are emerging on role Ubiquitin-Specific Proteases (USPs) development cancers, few data exist regarding OS. this context, RNA-sequencing analysis OS cells mesenchymal stem differentiated not into osteoblasts reveals increased expression four USPs...
Abstract The development of osteosarcoma, the most common malignant primary bone tumor is characterized by a vicious cycle established between proliferation and paratumor osteolysis. This osteolysis mainly regulated receptor activator nuclear factor κB ligand (RANKL). Preclinical studies have demonstrated that Rankl blockade soluble receptors an effective strategy to prevent osteolytic lesions leading osteosarcoma inhibition. A new therapeutic option could be directly inhibit expression...
Despite recent improvements in therapeutic management of osteosarcoma, ongoing challenges improving the response to chemotherapy warrants development new strategies improve overall patient survival. Among them, HSP90 is a molecular chaperone involved maturation and stability various oncogenic proteins leading tumor cells survival disease progression. We assessed antitumor properties synthetic inhibitor, PF4942847, alone or combination with zoledronic acid osteosarcoma.The effects PF4942847...