A5010733094- Pharmacogenetics and Drug Metabolism
- Liver physiology and pathology
- Drug-Induced Hepatotoxicity and Protection
- Liver Disease Diagnosis and Treatment
- Eicosanoids and Hypertension Pharmacology
- Carcinogens and Genotoxicity Assessment
- Drug Transport and Resistance Mechanisms
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Organ Transplantation Techniques and Outcomes
- Cancer Immunotherapy and Biomarkers
- Cutaneous Melanoma Detection and Management
- Metabolomics and Mass Spectrometry Studies
- Melanoma and MAPK Pathways
- RNA modifications and cancer
- DNA Repair Mechanisms
- Liver Disease and Transplantation
- Aquaculture disease management and microbiota
- Polyomavirus and related diseases
- Full-Duplex Wireless Communications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic function and diabetes
- Molecular Biology Techniques and Applications
- Mycotoxins in Agriculture and Food
- Physiological and biochemical adaptations
Inserm
2013-2025
Université de Rennes
2015-2024
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2013-2024
Centre National de la Recherche Scientifique
2011-2024
Centre Hospitalier Universitaire de Rennes
2008-2024
Université Rennes 2
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Foie, Métabolisme, Cancer
2011-2019
Université Européenne de Bretagne
2009-2015
Hôpital Pontchaillou
1993-2015
Cytokines are thought to cause the depression of cytochrome P-450 (CYP)-associated drug metabolism in humans during inflammation and infection. We have examined role five cytokines, i.e., interleukin-1 beta, interleukin-4, interleukin-6, tumor necrosis factor-alpha, interferon-gamma, on expression CYP1A2, CYP2C, CYP2E1, CYP3A, epoxide hydrolase primary human hepatocyte cultures. Steady state mRNA levels, as well ethoxyresorufin-O-deethylase nifedipine oxidation activities, which mainly...
Idiopathic pulmonary fibrosis is a devastating as yet untreatable disease. We demonstrated recently the predominant role of NLRP3 inflammasome activation and IL-1β expression in establishment inflammation mice.The contribution IL-23 or IL-17 was assessed using bleomycin model deficient mice.We show that IL-1β-induced lung injury leads to increased early IL-23p19, IL-17A IL-17F expression. Early IL-23p19 IL-17A, but not IL-17F, IL-17RA signaling are required for inflammatory response BLM...
Abstract This study reports on the characterization of B cells germinal center (GC)-like structures infiltrating salivary glands (SGs) patients with Sjögren’s syndrome. Eight two-color combinations were devised to characterize phenotype these in 11 SG specimens selected from biopsies obtained 40 syndrome and three normal tonsils. The 9G4 mAb, which recognizes V4.34-encoded autoAbs, enabled us identify autoreactive cells. Quantitative RT-PCR was used determine level mRNAs for...
Intrahepatic cholangiocarcinoma (ICC) is the second most common type of primary cancer in liver. ICC an aggressive with poor prognosis and limited therapeutic strategies. The identification new drug targets prognostic biomarkers important clinical challenge for ICC. presence abundant stroma a histological hallmark Given well-established role stromal compartment progression diseases, we hypothesized that relevant could be identified by analyzing By combining laser capture microdissection gene...
Cryopreserved hepatocytes from various animal species and human beings were tested for their ability to survive function in primary culture. The freeze/thaw protocol primarily designed rat was used with slight modifications the cells of all other species; it consisted suspending parenchymal Leibovitz L15 medium containing 10% fetal calf serum 16% dimethyl sulfoxide. After transient storage at 4 degrees C cell suspensions transferred -20 then -70 before being plunged liquid nitrogen....
CD95 ligand (CD95L) is expressed by immune cells and triggers apoptotic death. Metalloprotease-cleaved CD95L (cl-CD95L) released into the bloodstream but does not trigger signaling. Hence, pathophysiological role of cl-CD95L remains unclear. We observed that skin-derived endothelial from systemic lupus erythematosus (SLE) patients after cleavage, promoted T helper 17 (Th17) lymphocyte transmigration across barrier at expense regulatory cells. cell migration relied on a direct interaction...
Merkel cell carcinoma (MCC) is a rare but aggressive skin cancer involving cells. Recently, new human polyomavirus was implicated in MCC, being present 80% of the samples analyzed. In virus-positive (MCPyV) clonally integrated into patients DNA, and carries mutations its large T antigen, leading to truncated protein. non-symptomatic tissue MCPyV can reside at very low levels. MCC also associated with older age, immunosuppression sun exposure. However, link solar exposure remains unknown, as...
Aurora kinases belong to a conserved family of serine/threonine key regulators cell cycle progression. Aurora-A and Aurora-B are expressed in somatic cells involved mainly mitosis while Aurora-C is during spermatogenesis oogenesis meiosis. hardly detectable normal cells. However all three overexpressed many cancer lines. possesses an oncogenic activity does not. Here we investigated whether such activity. We report that overexpression induces abnormal division resulting centrosome...
Melanoma is a highly aggressive cancer endowed with unique capacity of rapidly metastasizing, which fundamentally driven by aberrant cell motility behaviors. Discovering "migrastatics" targets, specifically controlling invasion and dissemination melanoma cells during metastasis, therefore primary importance. Here, we uncover the prominent expression plasma membrane TRPV2 calcium channel as distinctive feature tumors, directly related to metastatic dissemination. In vitro well in vivo,...
Osteosarcoma is medically defined as a bone-forming tumor with associated bone-degrading activity. There lack of knowledge about the network that generates overproduction bone. We studied early stage osteosarcoma development mice enduring periosteum injection cells at proximal third tibia. On day 7 (D7), activate over-synthesis bone-like material inside medulla. This bone quickly (D13) followed by degradation. Samples were characterized microfocus small-angle X-ray scattering (SAXS),...
The aryl hydrocarbon receptor (AhR) is involved in various processes such as cytochrome P450 (P450) 1A induction after xenobiotic exposure. It also considered to play a major role cell proliferation and differentiation. Recent evidences have suggested cross-talk between AhR functions the mitogen-activated protein kinase (MAPK) cascade. We now report that 1,4-diamino-2,3-dicyano-1,4-<i>bis</i>[2-aminophenylthio]butadiene (U0126), specific inhibitor of MAPK (MEK) MEK1/2, elicits marked...
The effects of interleukin (IL)-1 beta, IL-4, IL-6, tumor necrosis factor (TNF)-alpha, interferon (IFN)-alpha, IFN-gamma, and transforming growth (TGF)-beta 1 on cytochrome P-450 (CYP) 1A expression polycyclic aromatic hydrocarbon (PAH)-mediated induction in primary human hepatocyte cultures were determined. Most cytokines that previously found to decrease basal CYP could counteract PAH CYP1A mRNA its associated ethoxyresorufin-O-deethylation (EROD) activity. IL-1 beta TNF-alpha blocked...
Abstract The nucleotide excision repair (NER) pathway and its leading gene excision-repair cross-complementary 1 (ERCC1) have been shown to be up-regulated in hepatocellular carcinomas even the absence of treatment with chemotherapeutics. aim this study was determine mechanism involved NER regulation during liver cell growth observed carcinoma. Both activity ERCC1 expression were increased after exposure epidermal factor (EGF) cultured normal tumoral human hepatocytes. These increases...
Intrahepatic cholangiocarcinoma (iCCA) is a deadly liver primary cancer associated with poor prognosis and limited therapeutic opportunities. Active transforming growth factor beta (TGFβ) signaling hallmark of the iCCA microenvironment. However, impact TGFβ on transcriptome tumor cells has been poorly investigated. Here, we have identified specific signature genes commonly deregulated in cell lines, namely HuCCT1 Huh28. Novel coding noncoding targets were identified, including TGFβ-induced...
Clinical studies suggest that moderate alcohol consumption can have beneficial effects, in particular regarding cardiovascular events, insulin resistance, and type 2 diabetes. In this study, lean obese diabetic ob/ob mice were submitted or not to chronic ethanol intake via the drinking water for 6 months, which was associated with levels of plasma ethanol. Plasma alanine aminotransferase aspartate increased by intake. Ethanol progressively reduced gain body weight mice, but observed despite...
Adenosine triphosphate (ATP) has been described as a danger signal activating the NOD-like receptor-family protein 3 (NLRP3)-inflammasome leading to pro-inflammatory cytokine, interleukin (IL)-1β, release in lung. The NLRP3-inflammasome pathway previously be involved experimental collagen deposition and development of pulmonary fibrosis. aim present study was investigate role NLRP3 inflammasome P2X7 purinergic receptor activation human macrophages vitro by ATP. We showed that adenosine...
Abstract Background Mutations of BRAF, NRAS and c‐KIT oncogenes are preferentially described in certain histological subtypes melanoma linked to specific histopathological features. BRAF‐, MEK‐ KIT‐inhibitors led improvement overall survival patients harbouring mutated metastatic melanoma. Objectives To assess the prevalence types NRAS, MITF mutations cutaneous mucous correlate mutation status with clinicopathological features outcome. Methods Clinicopathological 108 samples 98 consecutive...
Fish gills represent a complex organ composed of several cell types that perform multiple physiological functions. Among these cells, ionocytes are implicated in the maintenance ion homeostasis. However, because ionocyte represents only small percent whole gill tissue, its specific transcriptome can be overlooked among numerous included gill. The objective this study is to better understand functions by comparing RNA expression type freshwater and seawater acclimated rainbow trout. To...