A5046597795- Endodontics and Root Canal Treatments
- Bone and Dental Protein Studies
- Immune Response and Inflammation
- dental development and anomalies
- Oral microbiology and periodontitis research
- Periodontal Regeneration and Treatments
- Dental materials and restorations
- Mesenchymal stem cell research
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Dental Radiography and Imaging
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Oral and Maxillofacial Pathology
- Wound Healing and Treatments
- Bone health and treatments
- Bone Tissue Engineering Materials
- Dental Trauma and Treatments
- Dental Implant Techniques and Outcomes
- Cancer Diagnosis and Treatment
- TGF-β signaling in diseases
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Proteoglycans and glycosaminoglycans research
- Dental Research and COVID-19
Hospices Civils de Lyon
2015-2024
Biologie Tissulaire et Ingénierie Thérapeutique
2015-2024
Centre National de la Recherche Scientifique
2014-2024
Université Claude Bernard Lyon 1
2015-2024
Fundação Faculdade de Odontologia
2004-2020
Institut de Génomique Fonctionnelle de Lyon
2008-2015
École Normale Supérieure de Lyon
2008-2014
Université de Lyon
2014
Université de Rennes
2013
Inserm
2005-2008
Gram-positive bacteria entering the dentinal tissue during carious process are suspected to influence immune response in human dental pulp. Odontoblasts situated at pulp/dentin interface first cells encountered by these and therefore could play a crucial role this response. In present study, we found that vitro-differentiated odontoblasts constitutively expressed pattern recognition receptor TLR1-6 9 genes but not TLR7, 8, 10. Furthermore, lipoteichoic acid (LTA), wall component of bacteria,...
Dental pulp is a dynamic tissue able to resist external irritation during tooth decay by using immunocompetent cells involved in innate and adaptive responses. To better understand the immune response of toward gram-negative bacteria, we analyzed biological mediators rat incisor experimentally inflamed either lipopolysaccharide (LPS) or saline solution (phosphate-buffered [PBS]). Untreated teeth were used as control. Expression pro- anti-inflammatory cytokines, chemokine ligands, growth...
Odontoblasts and fibroblasts are suspected to influence the innate immune response triggered in dental pulp by micro-organisms that progressively invade human tooth during caries process. To determine whether they differ their responses oral pathogens, we performed a systematic comparative analysis of odontoblast-like cell fibroblast TLR2-, TLR3-, TLR4-specific agonists (lipoteichoic acid [LTA], double-stranded RNA, lipopolysaccharide [LPS], respectively). Cells responded these differential...
OPINION article Front. Physiol., 11 November 2013Sec. Craniofacial Biology and Dental Research https://doi.org/10.3389/fphys.2013.00326
Transforming Growth Factor-betal (TGFβ1) is a potent modulator of tissue repair in various tissues. To analyze its role during human dental repair, we used thick-sliced teeth cultured as described previously (Magloire et al., 1996). The supply TGFβ1 to the pulp was accomplished by means small tube glued onto dentin. We show that this device allowed growth factor diffuse locally through dentinal tubules and bind cells present coronal opposite TGFβ1-delivery tube. filled with 20 ng/mL TGFβ1,...
Nanocomposite fibrin-based hydrogel with antibacterial and antibiofilm properties, safe for dental pulp mesenchymal stem cells.
Human odontoblasts trigger immune response s to oral bacteria that invade dental tissues during the caries process. To date, their ability regulate expression of nucleotide-binding domain leucine-rich repeat containing receptor NOD2 when challenged by Gram-positive is unknown. In this study, we investigated in healthy and inflamed human pulps bacteria, cultured odontoblast-like cells stimulated with lipoteichoic acid (LTA), a Toll-like (TLR) 2 agonist which specific for bacteria. We found...
In recent years, mesenchymal cell-based products have been developed to improve surgical therapies aimed at repairing human tissues. this context, the tooth has recently emerged as a valuable source of stem/progenitor cells for regenerating orofacial tissues, with easy access pulp tissue and high differentiation potential dental cells. International guidelines now recommend use standardized procedures cell isolation, storage expansion in culture ensure optimal reproducibility, efficacy...
TGF-beta1 released from dentin degraded by bacterial or iatrogenic agents is suspected to influence dental pulp response, including the modulation of cell migration. To determine consequences action on immune cells, we analyzed, immunohistochemistry, effect transdentinally diffusing their localization in a human tooth slice culture model. induced an accumulation HLA-DR-positive cells both odontoblast and subodontoblast layers stimulated zone. Together with HLA-DR, these co-expressed Factor...