A5025667256- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Electrospun Nanofibers in Biomedical Applications
- Extracellular vesicles in disease
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Hematopoietic Stem Cell Transplantation
- MicroRNA in disease regulation
- Proteoglycans and glycosaminoglycans research
- Pluripotent Stem Cells Research
- Erythrocyte Function and Pathophysiology
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Cancer Diagnosis and Treatment
- Renal and related cancers
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Muscle Physiology and Disorders
- Wound Healing and Treatments
- Blood transfusion and management
- Biomedical Ethics and Regulation
German Red Cross
2016-2025
Heidelberg University
2016-2025
University Hospital Heidelberg
2016-2025
University Medical Centre Mannheim
2015-2024
Medizinische Fakultät Mannheim
2018-2024
University of Mannheim
2005-2023
DRK-Blutspendedienst Baden-Württemberg - Hessen
2005-2022
Karlsruhe Institute of Technology
2020
Heidelberg University
2012-2019
Tissue Dynamics (Israel)
2013
Mesenchymal stem cells (MSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. Bone marrow (BM) was the first source reported to contain MSCs. However, use, BM may be detrimental due highly invasive donation procedure and decline MSC number differentiation potential with increasing age. More recently, umbilical cord blood (UCB), attainable by less method, introduced as an alternative Another is adipose tissue (AT). We compared MSCs derived from these...
Abstract Evidence has emerged that mesenchymal stem cells (MSCs) represent a promising population for supporting new clinical concepts in cellular therapy. However, attempts to isolate MSCs from umbilical cord blood (UCB) of full-term deliveries have previously either failed or been characterized by low yield. We investigated whether with MSC characteristics and multi-lineage differentiation potential can be cultivated UCB healthy newborns yields might maximized optimal culture conditions...
ABSTRACT Pattern recognition via Toll-like receptors (TLR) by antigen-presenting cells is an important element of innate immunity. We report that wild-type measles virus but not vaccine strains activate both human and murine TLR2, this a property the hemagglutinin (H) protein. The ability to TLR2 MV H protein abolished mutation single amino acid, asparagine at position 481 tyrosine, as found in attenuated strains, which for interaction with CD46, receptor these strains. activation stimulates...
Abstract Mesenchymal stromal cells (MSCs) are promising candidates for novel cell therapeutic applications. For clinical scale manufacturing, human factors from serum or platelets have been suggested as alternatives to fetal bovine (FBS). We previously shown that pooled (HS) and thrombin-activated platelet releasate in plasma (tPRP) support the expansion of adipose tissue-derived MSCs. Contradictory results with bone marrow (BM)-derived MSCs initiated a comprehensive comparison HS, tPRP,...
Abstract Background Mesenchymal stromal cells (MSCs) are attractive for cell-based therapies ranging from regenerative medicine and tissue engineering to immunomodulation. However, clinical efficacy is variable it unclear how the phenotypes defining bone marrow (BM)-derived MSCs as well donor characteristics affect their functional properties. Methods BM-MSCs were isolated 53 (25 female, 28 male; age: 13 80 years) donors analyzed by: (1) phenotype using flow cytometry cell size measurement;...
The spatial presentation of mechanical information is a key parameter for cell behavior. We have developed method polymerization control in which the differential diffusion distance unreacted cross-linker and monomer into prepolymerized hydrogel sink results tunable stiffness gradient at cell-matrix interface. This simple, low-cost, robust was used to produce polyacrylamide hydrogels with gradients 0.5, 1.7, 2.9, 4.5, 6.8, 8.2 kPa/mm, spanning vivo physiological pathological landscape....
Abstract MSCs are currently in focus regarding their clinical potential cell therapy and tissue engineering. However, most isolation expansion protocols for clinical-scale production of use fetal calf serum (FCS) as a supplement, which poses risk infections well immunological reactions. To find suitable FCS substitute, we investigated the effects pooled human AB (AB-HS) thrombin-activated platelet-rich plasma (tPRP) on adipose (AT-MSCs) with standard control medium. AT-MSCs 10 donors were...
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment myocardial infarction (MI) remains unexplored. This study was to assess regenerative potential hMSC origins and evaluate role CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation received respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, catheterization showed...
Hypoxic preconditioning was shown to improve the therapeutic efficacy of bone marrow-derived multipotent mesenchymal stromal cells (MSCs) upon transplantation in ischemic tissue. Given interest clinical applications umbilical cord blood-derived MSCs, we developed a specific hypoxic protocol and investigated its anti-apoptotic pro-angiogenic effects on blood MSCs undergoing simulated ischemia vitro by subjecting them hypoxia nutrient deprivation with or without preceding preconditioning. Cell...
The inherent immunomodulatory capacity of mesenchymal stem/progenitor cells (MSPCs) encouraged initiation multiple clinical trials. Release criteria for therapeutic MSPCs cover identity, purity and safety but appropriate potency assessment is often missing. Reports on functional heterogeneity created additional uncertainty regarding donor organ/source selection. We established a robust immunomodulation assay based pooling responder leukocytes to minimize individual immune response...
Abstract Hematopoietic stem cells (HSCs) in the bone marrow are able to differentiate into all types of blood and supply organism each day with billions fresh cells. They applied cure hematological diseases such as leukemia. The clinical need for HSCs is high there a demand being control multiply vitro . hematopoietic system highly proliferative thus sensitive anti-proliferative drugs chemotherapeutics. For many these suppression dose-limiting toxicity. Therefore, biomimetic 3D models HSC...
Mesenchymal stromal cells (MSCs) have been isolated from many tissues, including gestational tissue. To date, a study comparing the properties and suitability of these in cell-based therapies is lacking. In this study, we compared phenotype, proliferation rate, migration, immunogenicity, immunomodulatory capabilities human MSCs derived umbilical cord lining (CL-MSCs), blood (CB-MSCs), placenta (P-MSCs), Wharton's jelly (WJ-MSCs). Differences were noted differentiation, proliferation, with...
The number of Mesenchymal Stem/Stromal Cells (MSCs) in the human bone marrow (BM) is small compared to other cell types. BM aspirate concentration (BMAC) may be used increase numbers MSCs, but composition MSC subpopulations and growth factors after processing are unknown. purpose this study was assess enrichment stem/progenitor cells by two different commercial devices versus standard aspiration. 120 mL aspirated from iliac crest 10 male donors. Each sample processed simultaneously either...