A5080109321- Knee injuries and reconstruction techniques
- Shoulder Injury and Treatment
- Sports injuries and prevention
- Total Knee Arthroplasty Outcomes
- Osteoarthritis Treatment and Mechanisms
- Periodontal Regeneration and Treatments
- Tendon Structure and Treatment
- Lower Extremity Biomechanics and Pathologies
- Mesenchymal stem cell research
- Anesthesia and Pain Management
- Shoulder and Clavicle Injuries
- Bone fractures and treatments
- Pregnancy-related medical research
- Adhesion, Friction, and Surface Interactions
- Bone and Joint Diseases
- Foot and Ankle Surgery
- Orthopedic Infections and Treatments
- Biomedical Ethics and Regulation
- Inflammatory mediators and NSAID effects
- Trauma Management and Diagnosis
- Orthopedic Surgery and Rehabilitation
- Spine and Intervertebral Disc Pathology
- Sexual function and dysfunction studies
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Effects of Exercise
University of Colorado Denver
2019-2025
University of Colorado Anschutz Medical Campus
2021-2025
University of Colorado Health
2020-2025
Steadman Clinic
2020-2025
University of Colorado Boulder
2023-2024
University of Colorado System
2023
Stanford University
2011-2020
Stanford Medicine
2013-2020
Palo Alto Institute
2019
University of Utah
2017
Our laboratory has recently characterized a population of cells from adipose tissue, termed processed lipoaspirate (PLA) cells, which have multi-lineage potential similar to bone-marrow-derived mesenchymal stem (MSCs). This study is the first comparison PLA and MSCs isolated same patient. No significant differences were observed for yield adherent stromal growth kinetics, cell senescence, differentiation capacity, gene transduction efficiency. Adipose tissue an abundant easily procured...
Background: Clinical studies claim that platelet-rich plasma (PRP) shortens recovery times because of its high concentration growth factors may enhance the tissue repair process. Most these obtained PRP using different separation systems, and few analyzed content used as treatment. Purpose: This study characterized composition single-donor produced by 3 commercially available systems. Study Design: Controlled laboratory study. Methods: Five healthy humans donated 100 mL blood, which was...
Background: Previous studies have shown improvement in patellar tendinopathy symptoms after platelet-rich plasma (PRP) injections, but no randomized controlled trial has compared PRP with dry needling (DN) for this condition. Purpose: To compare clinical outcomes a single ultrasound-guided, leukocyte-rich injection versus DN. Study Design: Randomized trial; Level of evidence, 1. Methods: A total 23 patients on examination and MRI who had failed nonoperative treatment were enrolled to receive...
Background: Numerous studies have shown platelet-rich plasma (PRP) preparations differ with respect to the inclusion of certain blood components, which may affect host’s cellular response. Hypothesis: This study evaluated inflammatory effect Biomet GPS III leukocyte-rich PRP (LR-PRP) versus MTF Cascade leukocyte-poor (LP-PRP) after intratendinous injection in an animal model. The authors anticipated that LR-PRP would incite a greater acute response than LP-PRP. Study Design: Controlled...
The effect of platelet-rich plasma (PRP) on chondrocytes has been studied in cell and tissue culture, but considerably less attention given to the PRP synoviocytes. Fibroblast-like synoviocytes (FLS) compose 80% normal human synovium produce cytokines matrix metalloproteinases that can mediate cartilage catabolism.To compare effects leukocyte-rich (LR-PRP), leukocyte-poor (LP-PRP), red blood (RBC) concentrate, platelet-poor (PPP) FLS determine whether leukocyte erythrocyte concentrations...
We have isolated pluripotent mesenchymal progenitor cells in large numbers from liposuction aspirates (processed lipoaspirate or PLAs). This study examines the osteogenic potential of PLAs and bone marrow aspirate (BMAs), when exposed to either recombinant human morphogenetic protein (BMP)-2 (rh-BMP-2) adenovirus containing BMP-2 cDNA (Ad-BMP-2).Liposuction underwent proteolytic digestion obtain PLAs. After exposure exogenous rh-BMP-2 Ad-BMP-2 for four seven days, BMAs were assessed by...
Multipotential processed lipoaspirate (PLA) cells extracted from five human infrapatellar fat pads and embedded into fibrin glue nodules, were induced the chondrogenic phenotype using media. The remaining placed in osteogenic media transfected with an adenovirus carrying cDNA for bone morphogenetic protein-2 (BMP-2). We evaluated tissue-engineered cartilage vitro techniques by placing hind legs of severe combined immunodeficient mice. After six weeks, radiological histological analysis...
The purpose of this study was to evaluate the use adipose-derived stem cells (ADSCs) as a source for full-thickness cartilage repair in an animal model. Autologous ADSCs were isolated and induced with growth medium placed fibrin glue scaffold into 3-mm x 4-mm chondral defects rabbits negative controls. Specimens evaluated early healing using immunostaining, Western blotting, reverse transcriptase polymerase chain reaction, transfection Lac Z gene, quantitative assessment. Twelve 12 (100%)...
Background: Platelet-rich plasma (PRP) is an autologous blood product used to treat acute and chronic tendon, ligament, muscle injuries in over 86,000 athletes the United States annually. The World Anti-Doping Agency (WADA) banned intramuscular PRP injections competitive 2010 because of concerns that it may increase performance-enhancing growth factors. ban on was removed 2011 limited evidence for a systemic ergogenic effect PRP, but factors within remain prohibited. Purpose: To quantify...
Injuries to the anterior cruciate ligament (ACL) are common in athletic populations, particularly athletes participating football, soccer, and skiing.The purpose of this study was analyze National Collegiate Athletic Association (NCAA) Injury Surveillance System (ISS) men's football ACL injury database from playing seasons 2004-2005 through 2008-2009 determine incidence epidemiology complete NCAA athletes.Descriptive study.The ISS reviewed using specific code, "Anterior tear." The rate...
A small number of randomized controlled trials have found ultrasound-guided injection platelet-rich plasma (PRP) to be no more effective than saline for several tendinopathies; limited information exists patellar tendinopathy. In addition, different PRP formulations that produce varying concentrations leukocytes not been directly compared tendinopathy.To determine if a single injection, either leukocyte-rich (LR-PRP) or leukocyte-poor (LP-PRP), was superior the treatment The null hypothesis...
Background: The female anterior cruciate ligament may be more susceptible to injury than the male because of gender-specific expression receptors for relaxin, a collagenolytic hormone that promotes remodeling ligament. Purpose: This study was undertaken investigate whether collegiate athletes with elevated serum relaxin concentrations (SRC) sustain tears at an increased rate compared those lower SRC. Study Design: Cohort (prognosis); Level evidence, 2. Methods: From 2005 2010, 143 Division I...
The administration of amide-type local anesthetics to cartilaginous tissues has revealed potential chondrotoxicity.This study evaluated whether single doses 1% lidocaine, 0.25% bupivacaine, and 0.5% ropivacaine resulted in decreased chondrocyte viability or cartilage matrix degradation vitro.Controlled laboratory study.Monolayer human chondrocytes intact samples were cultured for 1 week media. Each drug was delivered a custom bioreactor over its clinical duration action. A Live/Dead...