Future cell-based therapies such as tissue engineering will benefit from a source of autologous pluripotent stem cells. For mesodermal engineering, one cells is the bone marrow stroma. The compartment contains several cell populations, including mesenchymal (MSCs) that are capable differentiating into adipogenic, osteogenic, chondrogenic, and myogenic However, procurement has potential limitations. An alternate adult obtainable in large quantities, under local anesthesia, with minimal...

Much of the work conducted on adult stem cells has focused mesenchymal (MSCs) found within bone marrow stroma. Adipose tissue, like marrow, is derived from embryonic mesenchyme and contains a stroma that easily isolated. Preliminary studies have recently identified putative cell population adipose stromal compartment. This population, termed processed lipoaspirate (PLA) cells, can be isolated human lipoaspirates and, MSCs, differentiate toward osteogenic, adipogenic, myogenic, chondrogenic...

Our laboratory has recently characterized a population of cells from adipose tissue, termed processed lipoaspirate (PLA) cells, which have multi-lineage potential similar to bone-marrow-derived mesenchymal stem (MSCs). This study is the first comparison PLA and MSCs isolated same patient. No significant differences were observed for yield adherent stromal growth kinetics, cell senescence, differentiation capacity, gene transduction efficiency. Adipose tissue an abundant easily procured...

The use of undifferentiated cells for cell-based tissue engineering and regeneration strategies represents a promising approach skeletal muscle repair. For such to succeed, readily available source myogenic precursor must be identified. We have previously shown that isolated from raw human lipoaspirates, called processed lipoaspirate cells, display multilineage mesodermal potential in vitro. Because liposuctioned fat is large quantities can harvested with low morbidity, it may an ideal stem...

In 2002, researchers at UCLA published a manuscript in Molecular Biology of the Cell describing novel adult stem cell population isolated from adipose tissue-the adipose-derived (ASC). Since that time, ASC has gone on to be one most popular populations currently being used field. With multilineage mesodermal potential and possible ectodermal endodermal potentials also, could conceivably an alternate pluripotent ES cells both lab clinic. this retrospective article, historical perspective is...

Human processed lipoaspirate (PLA) cells are multipotent stem cells, capable of differentiating into multiple mesenchymal lineages (bone, cartilage, fat, and muscle). To date, differentiation to nonmesodermal fates has not been reported. This study demonstrates that PLA can be induced differentiate early neural progenitors, which an ectodermal origin. Undifferentiated cultures human expressed markers characteristic such as neuron-specific enolase (NSE), vimentin, nuclear protein (NeuN)....

We have isolated pluripotent mesenchymal progenitor cells in large numbers from liposuction aspirates (processed lipoaspirate or PLAs). This study examines the osteogenic potential of PLAs and bone marrow aspirate (BMAs), when exposed to either recombinant human morphogenetic protein (BMP)-2 (rh-BMP-2) adenovirus containing BMP-2 cDNA (Ad-BMP-2).Liposuction underwent proteolytic digestion obtain PLAs. After exposure exogenous rh-BMP-2 Ad-BMP-2 for four seven days, BMAs were assessed by...

The use of stem cells for cell-based tissue-engineering strategies represents a promising alternative the repair cartilaginous defects. multilineage potential population putative mesodermal obtained from human lipoaspirates, termed processed lipoaspirate cells, was previously characterized. chondrogenic those confirmed with combination histological and molecular approaches. Processed under high-density micromass culture conditions, supplemented transforming growth factor-beta1, insulin,...

Human adipose tissue is an ideal source of autologous cells that both plentiful and easily obtainable in large quantities through the simple surgical procedure liposuction. The stromal vascular fraction contains a stem cell population, adipose-derived (ASCs), capable adipogenic, osteogenic, myogenic chondrogenic differentiation. These have already been recognized to possess great therapeutic potential engineering regeneration. In this study, we sought determine effect donor age on growth...

The purpose of this study was to evaluate the use adipose-derived stem cells (ADSCs) as a source for full-thickness cartilage repair in an animal model. Autologous ADSCs were isolated and induced with growth medium placed fibrin glue scaffold into 3-mm x 4-mm chondral defects rabbits negative controls. Specimens evaluated early healing using immunostaining, Western blotting, reverse transcriptase polymerase chain reaction, transfection Lac Z gene, quantitative assessment. Twelve 12 (100%)...

In 2001, researchers at the University of California, Los Angeles, described isolation a new population adult stem cells from liposuctioned adipose tissue that they initially termed Processed Lipoaspirate Cells or PLA cells. Since then, these have been renamed as Adipose-derived Stem ASCs and gone on to become one most popular populations in fields cell research regenerative medicine. Thousands articles now describe use variety animal models, including bone regeneration, peripheral nerve...

We performed a pilot study to investigate the ability of human adipose derived, multipotent stem cells be delivered and survive within bladder urethral smooth muscle.Lipoaspirate was acquired from female patients undergoing liposuction. The lipoaspirate processed yield pluripotent population (PLA) cells. For tissue delivery PLA were fluorescent labeled suspended in Hanks' balanced salt solution (Sigma Chemical Co., St. Louis, Missouri). To assess viability multiple animal models 8 Rnu...

Progenitor cells capable of induction into multiple mesenchymal lineages have been isolated from human liposuction aspirates. These cells, named processed lipoaspirate previously shown in vitro osteogenic capacity. The purpose this study was to examine the vivo bone capacity morphogenetic protein-2 (BMP-2)-transduced using adipose tissue harvest sites.Processed extracted abdominal and buttock aspirates (n = 5) infrapatellar fat pads were placed media containing Dulbecco's Modified Eagle...

<i>Background/Aims:</i> A crucial step in providing clinically relevant applications of cardiovascular tissue engineering involves the identification a suitable cell source. The objective this study was to identify exogenous and endogenous parameters that are critical for differentiation human adipose stem cells (hASCs) into cells. <i>Methods:</i> hASCs were isolated from lipoaspirate samples, analyzed, subjected two protocols. <i>Results:</i> As shown by...

Mesenchymal stem cells derived from human liposuction aspirates, termed processed lipoaspirate cells, have been utilized as cellular delivery vehicles for the induction of bone formation in tissue engineering and gene therapy strategies. In this study, we sought to evaluate efficacy morphogenetic protein (BMP)-2-producing adipose-derived inducing a posterolateral spine fusion an athymic rat model.Single-level (L4-L5) intertransverse spinal arthrodesis was attempted with use type-I collagen...

In Brief Study Design. Rat spinal fusion model. Objective. To compare the efficacy of human adipose tissue-derived mesenchymal stem cells (HATDMSCs) and bone marrow-derived (HBMDMSCs) transduced with an adenovirus containing cDNA for morphogenetic proteins (BMP)-2 inducing in athymic rat Summary Background Data. Recombinant BMPs have successfully induced clinical trials. However, large doses are required adequate repair. Regional gene therapy may deliver to specific anatomic sites more...

One potential treatment option for severe vocal fold scarring is to replace the cover layer with a tissue-engineered structure containing autologous cells. As first step toward that goal, we sought develop three-dimensional cell-populated matrix resembling layers of lamina propria and epithelium.Basic science investigation.Adipose-derived stem cells were cultured in fibrin hydrogels various growth factors. At end culture period, matrices sectioned labeled immunomarkers identify cell...

Recombinant human bone morphogenetic protein-2 (rhBMP2) has been shown to induce both in vitro osteogenic differentiation and vivo formation, with the capacity of rhBMP2 elicit repair numerous bony defects (calvaria, spinal fusion, femora, so on) well documented. In addition, approved by Food Drug Administration (FDA) for selected indications. Despite fact that healing is often achieved, challenge still remains optimize therapeutic use rhBMP2. One avenue may be through combination stem cells...

In 2001, researchers at the University of California, Los Angeles, described isolation a new population adult stem cells from liposuctioned adipose tissue that they initially termed Processed Lipoaspirate Cells or PLA cells. Since then, these have been renamed as Adipose-derived Stem ASCs and gone on to become one most popular populations in fields cell research regenerative medicine. Thousands articles now describe use variety animal models, including bone regeneration, peripheral nerve...

In Brief Adipose-derived stem cells (ADSCs) hold promise for use in tissue engineering. Despite growing enthusiasm of ADSCs, there is limited research that has examined their behavior different vitro and vivo systems. The purpose our study was to evaluate the effect extracellular matrix structure composition on osteogenic differentiation by comparing marker expression ADSCs grown under 2-dimensional or 3-dimensional cell culture conditions. Group 1 (2-D) included raised conventional...

Bone marrow- and adipose tissue-derived stromal cells (BMSCs ASCs, respectively) exhibit a similar capacity for osteogenic differentiation in vitro, but it is unclear whether they share common process, because originate from different tissues. The aim of this study was to explore BMSC ASC by focusing on the expression extracellular matrix–related genes (ECMGs), which play crucial role osteogenesis bone tissue regeneration vivo. We characterized gene profiles BMSCs ASCs using custom...