A5036621427- Osteoarthritis Treatment and Mechanisms
- Vitamin D Research Studies
- Cytokine Signaling Pathways and Interactions
- Bone Metabolism and Diseases
- TGF-β signaling in diseases
- Reproductive System and Pregnancy
- Inflammatory mediators and NSAID effects
- Bone health and treatments
- Vitamin C and Antioxidants Research
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- HIV-related health complications and treatments
- Muscle Physiology and Disorders
- Adrenal Hormones and Disorders
- Virus-based gene therapy research
- Tendon Structure and Treatment
- NF-κB Signaling Pathways
- Pregnancy and preeclampsia studies
- Periodontal Regeneration and Treatments
- Prostate Cancer Treatment and Research
- Heterotopic Ossification and Related Conditions
- HIV Research and Treatment
- Proteoglycans and glycosaminoglycans research
- Adenosine and Purinergic Signaling
- Cell Adhesion Molecules Research
University of Southern California
2018-2024
Southern California University for Professional Studies
2024
Nanjing Medical University
2018
University of California, Los Angeles
2002-2017
Orthopaedic Hospital
2010-2014
Oklahoma State University Center for Health Sciences
2008
George Washington University
2002
Johns Hopkins University
2002
ABSTRACT Brain microvascular endothelial cells (BMVECs) present an incomplete barrier to human immunodeficiency virus type 1 (HIV-1) neuroinvasion. In order clarify the mechanisms of HIV-1 invasion, we have examined uptake and transcellular penetration in vitro BMVEC model. No evidence productive infection was observed by luciferase, PCR, reverse transcriptase assays. Approximately 1% viral RNA infectious penetrated without disruption tight junctions. The upregulated ICAM-1 on plasma...
Vitamin D insufficiency is a global health issue. Although classically associated with rickets, low vitamin levels have also been linked to aberrant immune function and problems such as inflammatory bowel disease (IBD). To test the hypothesis that impaired status predisposes IBD, 8-wk-old C57BL/6 mice were raised from weaning on D-deficient or D-sufficient diets then treated dextran sodium sulphate (DSS) induce colitis. showed decreased serum of precursor 25-hydroxyvitamin D3 (2.5 ± 0.1 vs....
The vitamin D-activating enzyme 1α-hydroxylase (CYP27B1) and D receptor (VDR) support anti-inflammatory responses to in many tissues. Given the high basal expression of CYP27B1 VDR trophoblastic cells from placenta, we hypothesized that effects may be particularly important this organ. Pregnant wild type (WT) mice i.p. injected with LPS showed elevated mouse Cyp27b1 (4-fold) (6-fold). Similar results were also obtained after ex vivo treatment WT placentas LPS. To assess functional impact...
We examined the potential of ex vivo gene therapy to enhance bone repair using lentiviral vectors encoding either enhanced green fluorescent protein (EGFP) as a reporter or morphogenetic protein-2 (BMP-2) downstream cytomegalovirus immediate early (CMV) promoter murine leukemia virus long terminal repeat (RhMLV) derived from retrovirus adapted replicate in rhesus macaque. In vitro, rat marrow stromal cells (BMSCs) transduced with Lenti-CMV-EGFP Lenti-RhMLV-EGFP demonstrated over 90%...
Lung cancer metastases to bone produce a primarily mixed osteolytic/osteoblastic lesion. The purpose of this study was determine if blockade both pathways would inhibit the formation these lesions in bone. Inhibition osteoblastic lesion with noggin and osteolytic RANK:Fc successful treatment strategy progression lung bone.Approximately 9-30% patients develop metastases, leading significant morbidity mortality. A549 is non-small-cell (NSCLC) line that produces metastatic We sought key...
Mesenchymal stem cells derived from human liposuction aspirates, termed processed lipoaspirate cells, have been utilized as cellular delivery vehicles for the induction of bone formation in tissue engineering and gene therapy strategies. In this study, we sought to evaluate efficacy morphogenetic protein (BMP)-2-producing adipose-derived inducing a posterolateral spine fusion an athymic rat model.Single-level (L4-L5) intertransverse spinal arthrodesis was attempted with use type-I collagen...
Abstract Epidemiology has linked vitamin D deficiency with preeclampsia in humans. We hypothesized that low status pregnant mice may lead to symptoms of preeclampsia. Female BL6 were raised on D-sufficient or -deficient diets from weeks 4 age and then mated males at week 8. The resulting either allowed deliver pups monitored for blood pressure (BP) weight offspring euthanized day 14 18 gestation (E14 E18) analysis serum, placental/kidney tissues, fetuses. At E14 serum concentrations...
Abstract Epigenetic mechanisms guiding articular cartilage regeneration and age‐related disease such as osteoarthritis (OA) are poorly understood. STAT3 is a critical age‐patterned transcription factor highly active in fetal OA chondrocytes, but the context‐specific role of regulating epigenome cells remain elusive. In this study, DNA methylation profiling was performed across human chondrocyte ontogeny to build an epigenetic clock establish association between CpG age. Exposure adult...
We investigated 18 AIDS hearts (5 with and 13 without cardiomyopathy) by using immunocytochemistry computerized image analysis regarding the roles of HIV-1 proteins tumor necrosis factor ligands in HIV cardiomyopathy (HIVCM). HIVCM cardiomyocyte apoptosis were significantly related to each other expression inflammatory cells gp120 factor-α. In heart, active caspase 9, a component mitochondrion-controlled apoptotic pathway, elements death receptor-mediated factor-α Fas ligand, expressed...
Objective Human adult articular cartilage (AC) has little capacity for repair, and joint surface injuries often result in osteoarthritis (OA), characterised by loss of matrix, hypertrophy chondrocyte apoptosis. Inflammation mediated interleukin (IL)-6 family cytokines been identified as a critical driver proarthritic changes mouse human joints, resulting feed-forward process driving expression matrix degrading enzymes IL-6 itself. Here we show that signalling through glycoprotein 130...
In order to accelerate the tendon-bone healing processes and achieve efficient osteointegration between tendon graft bone tunnel, we aim design bioactive electrospun nanofiber membranes combined with stem/progenitor cells (TSPCs) promote osteogenic regeneration of interface.In this study, polycaprolactone (PCL), PCL/collagen I (COL-1) hybrid membranes, poly(dopamine) (PDA)-coated PCL PDA-coated PCL/COL-1 were successfully fabricated by electrospinning. The biochemical characteristics...
Adult mammals are incapable of multitissue regeneration, and augmentation this potential may shift current therapeutic paradigms. We found that a common co-receptor interleukin 6 (IL-6) cytokines, glycoprotein 130 (gp130), serves as major nexus integrating various context-specific signaling inputs to either promote regenerative outcomes or aggravate disease progression. Via genetic pharmacological experiments in vitro vivo, we demonstrated tyrosine 814 (Y814) within gp130 cellular stress...
With the increasing incidence of osteoarthritis (OA) in elderly patients and challenges posed by its multifaceted pathogenesis developing effective treatments, this study explores protein loading mode Progranulin (PGRN) therapeutic potential for treating OA. GelMA hydrogels prepared with different concentrations PGRN, employing AC-PEG-NHS as a crosslinking agent drug encapsulation, were used to test sustained release effect composite hydrogel delivery system. Additionally, we conducted vitro...
25-Hydroxyvitamin D (25D) circulates bound primarily to serum vitamin binding protein (DBP), with DBP showing higher affinity for 25D3 than 25D2. We therefore hypothesized that D2 (D2) promotes levels of unbound 25D (free 25D), different functional responses, relative D3 (D3). Week 3 C56BL/6 mice were placed on diets containing either or alone (both 1000 IU/kg). At week 8 and 16, had only 25D2 in circulation (26.6 ± 1.9 33.3 4.4 ng/mL), (28.3 2.0 31.7 2.1 ng/mL). (44.5 6.4 vs 62.4 11.6...
Articular cartilage injury impairs joint function and necessitates orthopedic intervention to restore the structure of cartilage. Extracellular matrix (ECM) scaffolds derived from bone marrow mesenchymal stem cells (BMSCs) can effectively promote cell adhesion, proliferation, chondrogenesis. However, pre-shaped ECM have limited applicability due their poor fit with irregular surface most articular defects. In this study, we fabricated an injectable active hydrogel autologous BMSCs-derived by...
Abstract Background Due to the unclear pathogenesis of osteoarthritis (OA), effective treatment for this ailment is presently unavailable. Accumulating evidence points chondrocyte senescence as a key driver in OA development. This study aims identify OA-specific microRNAs (miRNAs) targeting alleviate progression. Methods We screened and identified miRNAs differentially expressed normal cartilage, then confirmed impact miR-653-5p on functions phenotypes through vitro experiments with...
Abstract Growth of long bones and vertebrae is maintained postnatally by a long-lasting pool progenitor cells. Little known about the molecular mechanisms that regulate output maintenance cells give rise to mature cartilage. Here we demonstrate postnatal chondrocyte-specific deletion transcription factor Stat3 results in severely reduced proliferation coupled with increased hypertrophy, growth plate fusion, stunting signs progressive dysfunction articular This effect dimorphic, females more...
Inhibition of hedgehog (HH) signaling prevents cartilage degeneration and promotes repair in animal models osteoarthritis (OA). This study, undertaken OA human articular cartilage, was designed to explore whether kappa opioid receptor (KOR) modulation via the inhibition HH may have therapeutic potential for achieving disease-modifying activity OA.Primary synovial tissue samples from patients with knee undergoing total joint replacement healthy subjects were obtained National Disease Research...
Osteoarthritis (OA) impacts hundreds of millions people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for development OA. Current cartilage repair strategies moderately effective at reducing pain but often replace damaged tissue biomechanically inferior fibrocartilage. Here we describe development, transcriptomic ontogenetic characterization quality assessment...