A5057634885- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Cytokine Signaling Pathways and Interactions
- Knee injuries and reconstruction techniques
- Pregnancy and preeclampsia studies
- Drug Transport and Resistance Mechanisms
- Muscle Physiology and Disorders
- Periodontal Regeneration and Treatments
- Congenital heart defects research
- Proteoglycans and glycosaminoglycans research
- Bone Metabolism and Diseases
- Inflammatory mediators and NSAID effects
- Silk-based biomaterials and applications
- Cancer-related molecular mechanisms research
- TGF-β signaling in diseases
- CRISPR and Genetic Engineering
- Neuropeptides and Animal Physiology
- Pregnancy and Medication Impact
- Reproductive System and Pregnancy
- Receptor Mechanisms and Signaling
- Total Knee Arthroplasty Outcomes
- Pharmacological Effects and Toxicity Studies
- Hair Growth and Disorders
University of Southern California
2016-2025
Southern California University for Professional Studies
2024
Gomel State Medical University
2024
LAC+USC Medical Center
2024
Broad Center
2010-2023
Keck Hospital of USC
2019-2022
University of California, Los Angeles
2010-2018
Los Angeles Medical Center
2017
University of California System
2015
Los Angeles City College
2015
Our understanding of how mesodermal tissue is formed has been limited by the absence specific and reliable markers early mesoderm commitment. We report that commitment from human embryonic stem cells (hESCs) initiated epithelial-to-mesenchymal transition (EMT) as shown gene expression profiling reciprocal changes in cell surface proteins, EpCAM/CD326 NCAM/CD56. Molecular functional assays reveal earliest CD326 − CD56 + cells, generated hESCs presence activin A, BMP4, VEGF, FGF2, represent a...
Unlike bone tissue, articular cartilage regeneration has not been very successful and many challenges ahead. We have previously developed injectable hydrogels using photopolymerizable chitosan (MeGC) that supported growth of chondrocytes. In this study, we demonstrate a biofunctional hydrogel for specific use in by conjugating transforming factor-β1 (TGF-β1), well-documented chondrogenic factor, to MeGC impregnating type II collagen (Col II), one the major cartilaginous extracellular matrix...
Placental ATP binding cassette (ABC) transporters protect placental and fetal tissues by effluxing xenobiotics endogenous metabolites. We have investigated the effects of cytokines survival/growth factors, implicated in various pathologies, on ABC transporter expression function primary trophoblast cells. Treatment term trophoblasts vitro with tumor necrosis factor-α (TNF-α) or interleukin (IL)-1β decreased mRNA protein apical ABCB1/multidrug resistance gene product 1 (MDR1) ABCG2/breast...
ATP-binding cassette (ABC) efflux transporters are expressed in the human placenta where they thought to help protect fetus from xenobiotics. To evaluate models for analysis of ABC transporter function and regulation placenta, we have characterized expression activity multidrug resistance (MDR) 1/P glycoprotein (Pgp), MDR3/Pgp, breast cancer protein (BCRP), proteins 1 2 (MRPs 1, 2) differentiating primary trophoblast cells BeWo Jar cell lines. Real-time PCR immunoblotting were used mRNA...
Joint injury and osteoarthritis affect millions of people worldwide, but attempts to generate articular cartilage using adult stem/progenitor cells have been unsuccessful. We hypothesized that recapitulation the human developmental chondrogenic program pluripotent stem (PSCs) may represent a superior approach for restoration. Using laser-capture microdissection followed by microarray analysis, we first defined surface phenotype (CD166low/negCD146low/negCD73+CD44lowBMPR1B+) distinguishing...
The efflux pump ATP binding cassette superfamily member G2 (ABCG2)/breast cancer resistance protein (BCRP) is highly expressed in human placenta. We have investigated the role of BCRP protection placental trophoblasts from apoptosis and its expression idiopathic fetal growth restriction, a condition associated with abnormal apoptosis. Inhibition activity selective inhibitor Ko143 augmented cytokine (tumor necrosis factor-alpha/interferon-gamma)-induced phosphatidylserine externalization...
While adult heart muscle is the least regenerative of tissues, embryonic cardiomyocytes are proliferative, with stem (ES) cells providing an endless reservoir. In addition to secreted factors and cell-cell interactions, extracellular microenvironment has been shown play important role in cell lineage specification, understanding how scaffold elasticity influences cardiac differentiation crucial tissue engineering. Though previous studies have analyzed matrix on function differentiated...
We developed strategies to stabilize TGF-β1 signaling in visible blue-light inducible chitosan hydrogel systems for specific use cartilage regeneration.
Abstract Tissue regeneration is a process that recapitulates and restores organ structure function. Although previous studies have demonstrated wound-induced hair neogenesis (WIHN) in laboratory mice ( Mus ), the limited to center of wound unlike those observed African spiny Acomys ) mice. mechanics been implicated as an integral part tissue morphogenesis. Here, we use WIHN model investigate mechanical molecular responses mice, report these models demonstrate opposing trends spatiotemporal...
Abstract Epigenetic mechanisms guiding articular cartilage regeneration and age‐related disease such as osteoarthritis (OA) are poorly understood. STAT3 is a critical age‐patterned transcription factor highly active in fetal OA chondrocytes, but the context‐specific role of regulating epigenome cells remain elusive. In this study, DNA methylation profiling was performed across human chondrocyte ontogeny to build an epigenetic clock establish association between CpG age. Exposure adult...
With the increasing incidence of osteoarthritis (OA) in elderly patients and challenges posed by its multifaceted pathogenesis developing effective treatments, this study explores protein loading mode Progranulin (PGRN) therapeutic potential for treating OA. GelMA hydrogels prepared with different concentrations PGRN, employing AC-PEG-NHS as a crosslinking agent drug encapsulation, were used to test sustained release effect composite hydrogel delivery system. Additionally, we conducted vitro...
Multipotent mesenchymal stem cells (MSCs) have enormous potential in tissue engineering and regenerative medicine. However, until now, their development for clinical use has been severely limited as they are a mixed population of with varying capacities lineage differentiation formation. Here, we identify receptor tyrosine kinase-like orphan 2 (ROR2) cell surface marker expressed by those MSCs an enhanced capacity cartilage We generated clonal human MSC populations chondrogenesis. ROR2 was...
Tissue-specific gene expression defines cellular identity and function, but knowledge of early human development is limited, hampering application cell-based therapies. Here we profiled 5 distinct cell types at a single fetal stage, as well chondrocytes 4 stages in vivo 2 during vitro differentiation. Network analysis delineated five tissue-specific modules; these modules chromatin state defined broad similarities cartilage specification maturation vivo, including progressive silencing...
Rotator cuff tears are a common cause of shoulder pain and often necessitate operative repair. Muscle atrophy, fibrosis, fatty infiltration can develop after rotator tears, which may compromise surgical outcomes. This study investigated the regenerative potential 2 human adipose-derived progenitor cell lineages in murine model massive tears.Ninety immunodeficient mice were used (15 groups 6 mice). Mice assigned to 1 3 procedures: sham, supraspinatus infraspinatus tendon transection (TT), or...
Objective Human adult articular cartilage (AC) has little capacity for repair, and joint surface injuries often result in osteoarthritis (OA), characterised by loss of matrix, hypertrophy chondrocyte apoptosis. Inflammation mediated interleukin (IL)-6 family cytokines been identified as a critical driver proarthritic changes mouse human joints, resulting feed-forward process driving expression matrix degrading enzymes IL-6 itself. Here we show that signalling through glycoprotein 130...
In order to accelerate the tendon-bone healing processes and achieve efficient osteointegration between tendon graft bone tunnel, we aim design bioactive electrospun nanofiber membranes combined with stem/progenitor cells (TSPCs) promote osteogenic regeneration of interface.In this study, polycaprolactone (PCL), PCL/collagen I (COL-1) hybrid membranes, poly(dopamine) (PDA)-coated PCL PDA-coated PCL/COL-1 were successfully fabricated by electrospinning. The biochemical characteristics...
Adult mammals are incapable of multitissue regeneration, and augmentation this potential may shift current therapeutic paradigms. We found that a common co-receptor interleukin 6 (IL-6) cytokines, glycoprotein 130 (gp130), serves as major nexus integrating various context-specific signaling inputs to either promote regenerative outcomes or aggravate disease progression. Via genetic pharmacological experiments in vitro vivo, we demonstrated tyrosine 814 (Y814) within gp130 cellular stress...
Human embryonic stem cells (hESC) exist as large colonies containing tightly adherent, undifferentiated cells. Disaggregation of hESC single significantly affects their survival and differentiation, suggesting that adhesion mechanisms are critical for the assembly maintenance colonies. The goal these studies was to determine key extracellular matrix (ECM) components regulate growth hESC. Our demonstrate express a specific subtype laminin (laminin-511) nidogen-1. addition purified protein...