A5034347864- Hematopoietic Stem Cell Transplantation
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- T-cell and B-cell Immunology
- Congenital heart defects research
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Polyomavirus and related diseases
- Cancer-related molecular mechanisms research
- Chronic Lymphocytic Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Neutropenia and Cancer Infections
- Circular RNAs in diseases
- Cardiac Fibrosis and Remodeling
- Hematological disorders and diagnostics
- Transplantation: Methods and Outcomes
- Antifungal resistance and susceptibility
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lymphoma Diagnosis and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Renal Transplantation Outcomes and Treatments
- Organic Food and Agriculture
- Cardiovascular Function and Risk Factors
- Platelet Disorders and Treatments
Kyoto University
2015-2025
The University of Tokyo
2012-2024
Osaka Metropolitan University
2022-2024
Tokyo Metropolitan University
2022-2024
Science Council of Japan
2019-2024
Metropolitan University
2024
Universidad Metropolitana
2023
Osaka City University
2013-2022
Kyushu University
1992-2021
Nihon University
2010-2021
Embryonic stem (ES) cells are immortal and present the ability to self-renew while retaining their differentiate. In contrast, most primary possess a limited proliferative potential, when this is exhausted, undergo an irreversible growth arrest termed senescence. cells, senescence can be also triggered by variety of stress which ES highly refractory. Here authors report that capacity murine closely correlates with high activity different glycolytic enzymes, elevated flux, low mitochondrial...
A significant increase in congestive heart failure (CHF) was reported when the anti-ErbB2 antibody trastuzumab used combination with chemotherapy drug doxorubicin (Dox).The aim of present study to investigate role(s) miRNAs acute Dox-induced cardiotoxicity. Methods and resultsNeuregulin-1-ErbB signalling is essential for maintaining adult cardiac function.We found a reduction ErbB4 expression hearts mice after Dox treatment.Because proteasome pathway only partially involved expression, we...
Summary For successful chimeric antigen receptor T (CAR‐T) cell therapy, CAR‐T cells must be manufactured without failure caused by suboptimal expansion. In order to determine risk factors for manufacturing failure, we performed a nationwide cohort study in Japan and analysed patients with diffuse large B‐cell lymphoma (DLBCL) who underwent tisagenlecleucel production. We compared clinical between 30 cases that failed (7.4%) those succeeded ( n = 378). Among the failures, proportion of...
Although altered T cell function plays a part in immunosenescence, the mechanisms remain uncertain. Here we identify bona fide age-dependent PD-1(+) memory phenotype (MP) CD4(+) subpopulation that hardly proliferates response to receptor (TCR) stimulation and produces abundant osteopontin at cost of typical lymphokines. These cells demonstrate impaired repopulation Rag2(-/-) mice, but homeostatic proliferation gamma-ray-irradiated mice. also reveal unique molecular signature, including...
While adult heart muscle is the least regenerative of tissues, embryonic cardiomyocytes are proliferative, with stem (ES) cells providing an endless reservoir. In addition to secreted factors and cell-cell interactions, extracellular microenvironment has been shown play important role in cell lineage specification, understanding how scaffold elasticity influences cardiac differentiation crucial tissue engineering. Though previous studies have analyzed matrix on function differentiated...
Heart failure and atherosclerosis share the underlying mechanisms of chronic inflammation followed by fibrosis. A highly conserved microRNA (miR), miR-33, is considered as a potential therapeutic target for because it regulates lipid metabolism inflammation. However, role miR-33 in heart remains to be elucidated.To clarify involved failure.We first investigated expression levels miR-33a/b human cardiac tissue samples with dilated cardiomyopathy. Increased miR-33a was associated improving...
Both internal and external oxidative stresses act on DNA can induce carcinogenesis. 8-hydroxydeoxyguanosine (8-OHdG) is an indicator of stress it leads to transversion mutations 8-OHdG excision-repaired by glycosylase (OGG1). The purpose this study clarify the effect damage repair enzymes esophageal levels OGG1 were immunohistochemically evaluated in resected specimens, including squamous cell carcinoma (SCC) 97 patients with cancer. Higher normal epithelium associated a higher smoking index...
Tight control of cardiomyocyte proliferation is essential for the formation four-chambered heart. Although human mutation NKX2-5 linked to septal defects and atrioventricular conduction abnormalities, early lethality hemodynamic alteration in mutant models have caused controversy as whether Nkx2-5 regulates proliferation.In this study, we circumvented these limitations by atrial-restricted deletion Nkx2-5.Atrial-specific mutants died shortly after birth with hyperplastic working myocytes...
Abstract Adaptive thermogenesis is essential for survival, and therefore tightly regulated by a central neural circuit. Here, we show that microRNA (miR)-33 in the brain indispensable adaptive thermogenesis. Cold stress increases miR-33 levels hypothalamus −/− mice are unable to maintain body temperature cold environments due reduced sympathetic nerve activity impaired brown adipose tissue (BAT) Analysis of f/f dopamine-β-hydroxylase ( DBH )-Cre indicates importance Dbh -positive cells....
Familial hypercholesterolemia is an inherited disorder that remains underdiagnosed. Conventional genetic testing methods such as next-generation sequencing (NGS) or target PCR are based on the amplification process. Due to efficiency limits of polymerase and ligase enzymes, these usually short regions do not detect large mutations straightforwardly. This study combined long-read nanopore CRISPR-Cas9 system sequence DNA molecules without amplification. We originally designed optimized...
CCN1 (Cyr61) is an extracellular matrix-associated protein involved in cell proliferation and survival. bound to vascular smooth muscle cells (VSMCs) via integrins expressed VSMCs atherosclerotic lesions, suggesting involvement the regulation of (VSMC) atherosclerosis. We hypothesized that knockdown may inhibit VSMC suppress neointimal hyperplasia.We examined effect using rat cultured a balloon injury model. stimulated adhesion migration dose-dependent manner, this was blocked by antibody...
Background Despite recent progress with drug-eluting stents, restenosis and thrombosis after endovascular intervention are still major limitations in the treatment of cardiovascular diseases. These problems possibly caused by inappropriate inhibition neointimal formation retardation re-endothelialization on surface stents. miR-126 has been shown to have potential enhance vascular endothelial cell proliferation. Methods results We designed constructed a 27-nt double strand RNA (dsRNA)...
Nonalcoholic steatohepatitis (NASH) can lead to cirrhosis and hepatocellular carcinoma in their advanced stages; however, there are currently no approved therapies. Here, we show that microRNA (miR)-33b hepatocytes is critical for the development of NASH. miR-33b located intron sterol regulatory element-binding transcription factor 1 abundantly expressed humans, but absent rodents. knock-in (KI) mice, which have a sequence same as humans express similar exhibit NASH under high-fat diet...
Character education is increasingly recognized as a critical component in developing individuals who are not only academically proficient but also possess strong moral and ethical values. This study examines the integration of social studies, character education, Sustainable Development Goals (SDGs) within Japan's educational system, focusing on how these elements equip students with skills values necessary to address contemporary global challenges. Employing qualitative descriptive...
Endoplasmic reticulum (ER) stress pathways contribute to atherosclerosis progression. Recently, we developed Kyoto University Substance (KUS) 121, which selectively inhibits ATPase activities of valosin-containing protein (VCP), consequently conserving intracellular ATP consumption and mitigating ER stress. This study evaluated the efficacy KUS121 in atherosclerosis. was administered daily Apoe-/- mice fed a Western diet (WD) for 8 weeks. treatment resulted 40-50 % reduction Interestingly,...