A5090942576- Hematopoietic Stem Cell Transplantation
- Lymphoma Diagnosis and Treatment
- Bladder and Urothelial Cancer Treatments
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Developmental Biology and Gene Regulation
- Urinary and Genital Oncology Studies
- Renal cell carcinoma treatment
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Lung Cancer Treatments and Mutations
- Viral-associated cancers and disorders
- Urological Disorders and Treatments
- Crystallization and Solubility Studies
- Invertebrate Immune Response Mechanisms
- Medical Imaging and Pathology Studies
- Immune Cell Function and Interaction
- Prostate Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Chronic Myeloid Leukemia Treatments
- Urologic and reproductive health conditions
- Cytomegalovirus and herpesvirus research
- Acute Lymphoblastic Leukemia research
- Neutropenia and Cancer Infections
- Hematological disorders and diagnostics
Kyushu University
2024-2025
Hitachi (Japan)
2024
Ome Municipal General Hospital
2007-2023
University of Tsukuba
2003-2023
The University of Tokyo
2022-2023
Osaka City General Hospital
2009-2022
Tane General Hospital
1999-2020
Nippon Koei (Japan)
2020
Pharmaceuticals and Medical Devices Agency
2020
Tokyo Institute of Technology
2019
Stem cells are maintained in vivo by short-range signaling systems specialized microenvironments called niches, but the molecular mechanisms controlling physical space of stem cell niche poorly understood. In this study, we report that heparan sulfate (HS) proteoglycans (HSPGs) essential regulators germline (GSC) niches Drosophila melanogaster gonads. GSCs were lost both male and female gonads mutants deficient for HS biosynthesis. dally, a glypican, is expressed GSC responsible maintaining...
A slurry crystallization technique was used in cocrystal screening of two nonionizable pharmaceutical host compounds, stanolone and mestanolone, with 11 pharmaceutically acceptable guest acids. Crystallization performed simply by adding solvents to solid mixtures a guest, which had been prepared using lyophilization their dimethyl sulfoxide solution. Powder X-ray diffraction thermogravimetric/differential thermal analysis were identify new forms. Two resultant forms, l-tartaric acid 1:1...
Nanos (Nos) is one of the evolutionarily conserved proteins known to direct germ-line development. In Drosophila , maternal Nos protein maintains transcriptional quiescence in progenitors or pole cells repress ectopic expression somatic genes. Here we show that required establish and maintain identity by preventing apoptosis cell fate. The lacking were degraded during mid late embryogenesis. When was suppressed Df(3L)H99 some adopted fates. These expressed markers ectopically lost marker...
The many laboratory and diagnostic applications utilizing streptavidin as a molecular adaptor rely on its high affinity essentially irreversible interaction with biotin. However, there are situations where recovery of the biotinylated molecules is desirable. We have previously shown that poly(N-isopropylacrylamide) (PNIPAAm), temperature-sensitive polymer, can reversibly block biotin association polymer's conformation changes at lower critical solution temperature (LCST). Here, we...
Nanos (Nos) is an evolutionarily conserved protein essential for the survival of primordial germ cells. In Drosophila , maternal Nos partitions into pole cells and suppresses apoptosis to permit proper germ-line development. However, how this critical event regulated by has remained elusive. Here, we report that represses suppressing translation head involution defective ( hid ), a member RHG gene family required Caspase activation. addition, demonstrate acts in concert with another gene,...
The purpose of this study is to clarify the effects intestinal drug supersaturation on solubility-limited nonlinear absorption. Oral absorption a novel farnesyltransferase inhibitor (FTI-2600) from its crystalline free base and HCl salt was determined in dogs. To contribution improving absorption, vivo intraluminal concentration FTI-2600 after oral administration estimated pharmacokinetics data using physiologically based model. Dissolution precipitation characteristics biorelevant media...
Sex determination in the Drosophila germ line is regulated by both sex of surrounding soma and cell-autonomous cues. How primordial cells (PGCs) initiate sexual development via mechanisms unclear. Here, we demonstrate that, Drosophila, lethal (Sxl) gene acts autonomously PGCs to induce female development. Sxl transiently expressed during their migration gonads; this expression, which was detected only XX PGCs, necessary for assume a fate. Ectopic expression XY sufficient them enter oogenesis...
Immunomodulation induced by dasatinib is reportedly related to better prognosis in chronic myeloid leukemia (CML). However, the underlying mechanism has not yet been fully elucidated. The immunoprofiles of 63 patients phase CML were evaluated during treatment with a tyrosine kinase inhibitor (imatinib, n = 36; nilotinib, 9; dasatinib, 18). numbers CD56 + CD57 and CD3 cells increased significantly group. regulatory T-cells comparable among three groups. Dasatinib markedly enhanced natural...
Abstract Inhibitors of microtubule (MT) assembly or dynamics that target α/β-tubulin are widely exploited in cancer therapy and biological research. However, specific inhibitors the MT nucleator γ-tubulin would allow testing temporal functions during cell cycle yet to be identified. By evolving β-tubulin-binding drugs we now find glaziovianin A derivative gatastatin is a γ-tubulin-specific inhibitor. Gatastatin decreased interphase human cells without affecting number. inhibited mitotic...
Significance Metamorphosis is an important biological process by which animals alter their body structures to become sexually mature adults. We discovered that tyramine signaling through the β3-octopamine receptor plays essential role in producing steroid hormone ecdysone, critical for metamorphosis. Based on our observations, we propose monoamine acts downstream of a size checkpoint allows metamorphosis occur only when weight attained during larval development and nutrients are sufficiently...
In Drosophila, ligands of the Unpaired (Upd) family activate Janus kinase/signal transducers and activators transcription (JAK/STAT) pathway. The JAK/STAT pathway controls many developmental events, including multiple functions in ovary. These include an early role germarium for specification stalk cells a later vitellarium to pattern follicular epithelium surrounding each cyst. this latter role, graded activation specifies three distinct anterior cell fates, suggesting that Upd is morphogen...
A new benzophenone-diketopiperazine-type potent antimicrotubule agent was developed by modifying the structure of clinical candidate plinabulin (1). Although right-hand imidazole ring with a branched alkyl chain at 5-position in 1 critical for potency activity, we successfully substituted this moiety simpler 2-pyridyl converting left-hand from phenyl to benzophenone without decreasing potency. The resultant compound 6b (KPU-300) exhibited cytotoxicity, an IC50 value 7.0 nM against HT-29...
Men and women become infertile with age, but the mechanism of declining male fertility, more specifically, decrease in sperm quality, is not well known. Citrate synthase (CS) a core enzyme mitochondrial tricarboxylic acid (TCA) cycle, which directly controls cellular function. Extra-mitochondrial CS (eCS) produced abundant head; however, its role fertility unknown. We investigated eCS by producing eCs-deficient (eCs-KO) mice. The initiation first spike Ca2+ oscillation was substantially...
Abstract Background Histone post‐translational modification (PTM) is an important epigenomic regulation content and essential process regulating gene expression. lysine lactylation the newly identified histone PTM that utilizes lactyl moiety for its modification. Although considered outcome of Wardburg effects interconnection between cellular metabolism regulation, developmental contexts involving this are largely unknown. In study, we comprehensively observed during Drosophila oogenesis,...
S-adenosylmethionine (SAM) is the principal cellular donor of methyl moiety in methylation reaction and regulates gene expression by regulating methylation-related events, such as epigenetic status. Although SAM biosynthesis affects a variety biological phenomena including disease aging, whether cell-specific status present how it contributes to function are largely unknown. Here, we firstly showed that Drosophila germline gametogenesis has repressive through observation synthetase (Sam-S),...