A5016688742- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- DNA Repair Mechanisms
- Protist diversity and phylogeny
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Phytoplasmas and Hemiptera pathogens
- Aquaculture Nutrition and Growth
- Fungal and yeast genetics research
- Microbial infections and disease research
- Ubiquitin and proteasome pathways
- Genetic and Environmental Crop Studies
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Genetic and phenotypic traits in livestock
- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Coenzyme Q10 studies and effects
- RNA and protein synthesis mechanisms
Heidelberg University
2015-2021
Zhejiang University of Technology
2021
DKFZ-ZMBH Alliance
2015-2020
Jiangxi Academy of Forestry
2019
Center for Life Sciences
2014
National University of Singapore
2011-2012
Temasek Life Sciences Laboratory
2011-2012
Jatropha curcas is a potential plant species for biodiesel production. However, its seed yield too low profitable production of biodiesel. To improve the productivity, genetic improvement through breeding essential. A linkage map an important component in molecular breeding. We established first-generation using mapping panel containing two backcross populations with 93 progeny. mapped 506 markers (216 microsatellites and 290 SNPs from ESTs) onto 11 groups. The total length was 1440.9 cM...
Abstract Inhibitors of microtubule (MT) assembly or dynamics that target α/β-tubulin are widely exploited in cancer therapy and biological research. However, specific inhibitors the MT nucleator γ-tubulin would allow testing temporal functions during cell cycle yet to be identified. By evolving β-tubulin-binding drugs we now find glaziovianin A derivative gatastatin is a γ-tubulin-specific inhibitor. Gatastatin decreased interphase human cells without affecting number. inhibited mitotic...
MOZART1/Mzt1 is required for the localization of γ-tubulin complexes to microtubule (MT)-organizing centers from yeast human cells. Nevertheless, molecular function largely unknown. Taking advantage minimal MT nucleation system Candida albicans, we reconstituted interactions Mzt1, small complex (γ-TuSC), and receptors (γ-TuCRs) Spc72 Spc110 in vitro. With affinity measurements, domain deletion, swapping, show that Mzt1 bind distinct regions γ-TuSC. In contrast, both γ-TuSC interact with...
In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males ×10 females) of Asian seabass reconstruct monitor the change variations. all crosses, over 96.8% could be assigned their parents, indicating high power for parentage assignment. This study revealed several interesting results: (1)....
Cryo-electron microscopy recently resolved the structure of vertebrate γ-tubulin ring complex (γ-TuRC) purified from Xenopus laevis egg extract and human cells to near-atomic resolution. These studies clarified arrangement stoichiometry γ-TuRC components revealed that one molecule actin small protein MZT1 are embedded into complex. Based on this structural census core components, we developed a recombinant expression system for reconstitution purification insect cells. The recapitulates...
Traceability through physical labels is well established, but it not highly reliable as can be easily changed or lost. Application of DNA markers to the traceability food plays an increasingly important role for consumer protection and confidence building. In this study, we tested efficiency 16 polymorphic microsatellites their combinations tracing 368 fish four populations where they originated. Using maximum likelihood Bayesian methods, three most efficient were required assign over 95%...
Abstract The nucleation of microtubules from αβ-tubulin subunits is mediated by γ-tubulin complexes, which vary in composition across organisms. Aiming to understand how de novo microtubule formation achieved and regulated a minimal system, we here determined the cryo-electron microscopy structure heterotetrameric small complex (γ-TuSC) C. albicans at near-atomic resolution. Compared vertebrate ring (γ-TuRC), observed vastly remodeled interface between SPC/GCP-γ-tubulin spokes, stabilizes...
Whole mitochondrial genome sequence of Apriona swainsoni has a circular 15,412 bp. The contents A, G, T, and C are 38.90%, 9.29%, 35.81%, 15.99%, respectively. proportion coding sequences with total length 11, 141 bp is 72.29%, which encodes 3689 amino acids. Most protein-coding genes stopped TAN, COX1 COX2 taking the incomplete codon T as stop codon. (TA T) ubiquitous in insect genome. D-loop 735 bp, ranging from 14,677 to
The Chinese kukri snake Oligodon chinensis belongs to family Colubridae and is distributed in southern China North Vietnam. In this study, the total mitochondrial genome of O. was determined using next-generation sequencing.It a circular molecule 17,146 bp length contains 13 protein-coding genes, 22 transfer RNA 2 ribosomal control regions (CR1 CR2), with base composition A 33.6%, G 12.2%, T 25.6%, C 28.6%. Interestingly, special 136 non-coding region, which identified as repeated sequence...
Engineered zinc finger proteins have been widely used for gene editing and transcriptional regulation. Previously, various systems utilized in screening, but only targeting on sequences no more than 18 bp. Here, we present a novel fast screen method, which is one-hybrid system based gp8, phage-encoded inhibitor of DNA polymerase III. The whole screening process takes 3 days to identify batch proteins, could specifically tightly target 300 bp sequence mouse neurexin1- α promoter. specificity...