A5065631877- Head and Neck Cancer Studies
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Cancer Diagnosis and Treatment
- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Salivary Gland Tumors Diagnosis and Treatment
- Liver physiology and pathology
- Cancer Research and Treatments
- Oral and Maxillofacial Pathology
- Oral Health Pathology and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Virus-based gene therapy research
- Ear and Head Tumors
- Chemokine receptors and signaling
- Prostate Cancer Treatment and Research
- Proteoglycans and glycosaminoglycans research
- HER2/EGFR in Cancer Research
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Bone Tumor Diagnosis and Treatments
- Immunotherapy and Immune Responses
Ehime University
2016-2025
Kumamoto University
2024
Renesas Electronics (United States)
2009
Northwestern University
1999-2004
Tokushima University
1995-2003
Dokkyo Medical University
2003
We have established human oral-squamous-cancer cell lines, BHY and HN, derived from non-metastatic cancer metastatic respectively. examined the expression of matrix-degrading enzymes their inhibitors in these lines. Both lines expressed pro-matrix metalloproteinase (MMP)1, proMMP2, proMMP9, membrane-type MMP urokinase-type plasminogen activator. In addition to enzymes, cells secreted proMMP7 procathepsin L, while HN a large amount active MMP2. tissue inhibitor matrix metalloproteinase,...
DNA‐PK is a nuclear protein with serine/threonine kinase activity and forms complex consisting of the DNA‐PKcs heterodimer Ku70 Ku80 proteins. Recent laboratory experiments have demonstrated that formation one major pathways by which mammalian cells respond to DNA double‐strand breaks induced ionizing radiation. In this study, we evaluated relationship between expression levels DNA‐PKcs, proteins radiation sensitivity in oral squamous cell carcinoma (OSCC) lines OSCC patients treated...
Abstract BACKGROUND Almost all attempts at establishing prostate carcinoma cell lines have resulted in generation of cells that are androgen‐independent, including commonly used LNCaP which expresses androgen receptor (AR) and AR‐negative Du145 PC‐3. We attempted to clarify the mechanism(s) responsible for failure respond androgen. METHODS Cell LNCaP, CWR22R, PC‐3, Du145, CA7T2CL were examine AR promoter function with a reporter gene assay its methylation status by Southern blot, PCR...
The support mechanisms that are involved in lymph-node metastasis of oral squamous cell carcinoma (OSCC) remain largely unknown. Recent studies have demonstrated tumor cells express chemokine receptors and use chemokines to metastasize the target organ many malignancies humans. In this study, we examined expression function their OSCC. was assessed eight OSCC lines. CXCR3 mRNA protein were expressed all lines examined, while CXCR4 only HSC2, HSC3, Ca9-22 cells. Treatment with ligand for...
We investigated whether serum interleukin (IL)-8 reflects the tumor microenvironment and has prognostic value in patients with oral squamous cell carcinoma (OSCC).Fifty OSCC who received radical resection of their tumor(s) were enrolled. Preoperative sera measured for IL-8 by ELISA. Expression infiltration immune cells tissues analyzed an immunohistochemical staining surgical specimens.We found that disease-free survival (DFS) was significantly longer Stage I/II low levels compared to those...
Objective Several studies have suggested that an elevated neutrophil–lymphocyte ratio (NLR) is associated with a poorer prognosis in patients pancreatic cancer (PC). The correlations between the NLR and immunohistochemical (IHC) analysis regard to of PC remain be elucidated. By using IHC findings, we determined value as prognostic factor PC. Material methods We collected clinico-pathological data 28 consecutive who underwent surgical resection for January 2008 December 2012 at Jikei...
No clinically useful predictors of latent cervical lymph node metastasis (LNM) in early oral squamous cell carcinoma (OSCC) are available. In this study, we focused on the microRNAs (miRNAs) involved expression numerous genes and explored those associated with LNM OSCC (eOSCC). First, microarray RT-PCR analyses revealed a significant downregulation miR-375-3p primary eOSCC tissues LNM. Next, examined effects mimics growth migration four human lines that do not express miR-375-3p. The...
Abstract Molecular blockade of EGFR with either an MAb or TKI enhances the radiosensitivity human SCCs. In present study, we investigated whether treatment gefitinib (Iressa, ZD1839) improves response to radiotherapy in OSCC cell lines HSC2 and HSC3. We examined potential mechanisms that may contribute enhanced radiation induced by gefitinib. Growth inhibition was observed vitro A cooperative antiproliferative effect obtained when cancer cells were treated followed Cells a combination...
We examined the role of hepatocyte growth factor (HGF)/c-met system on invasion and metastasis oral squamous cell carcinoma (SCC) cells. In monolayer culture, exogenous HGF marginally affected SCC cells (BHY, HN, IH) human gingival epithelial (GE). type I collagen matrix, however, significantly enhanced invasive cancer (p < 0.05). detected expression c-met (HGF receptor) mRNA in all cells, but not fibroblasts (GF). Oral did secret protein into medium, GF secreted a large amount (15 ng/ml)....
We examined the gelatinolytic activity in human oral squamous-cell carcinoma tissues order to evaluate capability of intravasation and extravasation cancer cells. By a microdissection-zymography, we demonstrated separately activities cell nests stroma adjacent The activities, such as pro-matrix metalloproteinase (MMP)9 active-MMP2 most were much higher than those normal gingival epithelium. Moreover, metastatic cancers significantly non-metastatic (p<0.05). These results suggest that cells...
Previous studies have shown that low levels of p27(KIP1), an inhibitor G1 cyclin-dependent kinases (CDK), are associated with high aggressiveness and poor prognosis in a variety cancers. Decreased p27(KIP1) caused, at least part, by acceleration degradation Skp2 (S-phase kinase-associated protein 2) Jab1 (Jun activation domain-binding 1). This investigation was undertaken to examine whether the expression is correlated levels, how it clinically relevant oral squamous cell carcinoma (OSCC)....