A5011722136- Head and Neck Cancer Studies
- Cancer, Hypoxia, and Metabolism
- Salivary Gland Tumors Diagnosis and Treatment
- Oral and Maxillofacial Pathology
- TGF-β signaling in diseases
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Oral Health Pathology and Treatment
- Cancer Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Bone Tumor Diagnosis and Treatments
- Bladder and Urothelial Cancer Treatments
- Colorectal Cancer Treatments and Studies
- Salivary Gland Disorders and Functions
- Chemokine receptors and signaling
- Genetic factors in colorectal cancer
- Protease and Inhibitor Mechanisms
- Plant Pathogens and Fungal Diseases
- Ear and Head Tumors
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
Dokkyo Medical University
2016-2025
Japanese Urological Association
2024
Obayashi (Japan)
2021
Dokkyo University
2003-2018
Chukyo University
2015
Kyoto University
2001
Kobe University
2001
Zero to Three
2001
Tokushima University
1987-2000
Ministry of Health Labour and Welfare
1999
We have established human oral-squamous-cancer cell lines, BHY and HN, derived from non-metastatic cancer metastatic respectively. examined the expression of matrix-degrading enzymes their inhibitors in these lines. Both lines expressed pro-matrix metalloproteinase (MMP)1, proMMP2, proMMP9, membrane-type MMP urokinase-type plasminogen activator. In addition to enzymes, cells secreted proMMP7 procathepsin L, while HN a large amount active MMP2. tissue inhibitor matrix metalloproteinase,...
A Japanese blood donor (H. T.) and his brother (M. S.) are the first homozygous MkMk individuals described; their red cells lack, as expected, known antigens of MNSs group system also have no demonstrable MN-active Ss-active glycoproteins. Both a naturally occurring atypical antibody in serum. The serum H. T. is inhibited by MNSs-active glycoprotein preparations from normal erythrocytes.
We examined the role of hepatocyte growth factor (HGF)/c-met system on invasion and metastasis oral squamous cell carcinoma (SCC) cells. In monolayer culture, exogenous HGF marginally affected SCC cells (BHY, HN, IH) human gingival epithelial (GE). type I collagen matrix, however, significantly enhanced invasive cancer (p < 0.05). detected expression c-met (HGF receptor) mRNA in all cells, but not fibroblasts (GF). Oral did secret protein into medium, GF secreted a large amount (15 ng/ml)....
<b>Background:</b> Several animal models for human ulcerative colitis (UC) associated neoplasia have been reported. However, most neoplasias developed in these morphological and genetic characteristics different from UC neoplasia. <b>Aims:</b> To establish a new model p53 deficient mice by treatment with dextran sulphate sodium (DSS). <b>Methods:</b> DSS was induced homozygous (p53<sup>−/−</sup>-DSS), heterozygous (p53<sup>+/−</sup>-DSS) wild-type (p53<sup>+/+</sup>-DSS) 4% DSS. Numbers of...
We examined the gelatinolytic activity in human oral squamous-cell carcinoma tissues order to evaluate capability of intravasation and extravasation cancer cells. By a microdissection-zymography, we demonstrated separately activities cell nests stroma adjacent The activities, such as pro-matrix metalloproteinase (MMP)9 active-MMP2 most were much higher than those normal gingival epithelium. Moreover, metastatic cancers significantly non-metastatic (p<0.05). These results suggest that cells...
Abstract The balance between matrix metalloproteinases and their inhibitors is a critical factor which affects tumor invasion metastasis. We have established rat bladder carcinoma cell line, LMC19, tumorigenic, invasive metastatic to the retroperitoneal lymph nodes lungs in nude mice. LMC19 cells secrete pro‐gelatinases A B as well tissue of metalloproteinase (TIMP1 TIMP2). conducted present study determine whether or not over‐expression TIMP1 TIMP2 can affect potential cells. transfected...
Abstract Nhdmerous studies have reported a correlation between the production of gelatinases A and B by cancer cells anhd invasive metastatic potential. It has been suggested that expression gelatinase (72‐kDa type IV collagenase) is associated more closely with phenotype malignant in vitro vivo than (92‐kDa collagenase). We established rat bladder carcinoma cell line, MYU3L, which tumorigenic locally but not to distal organs nude mice. The MYU3L line secretes pro‐gelatinase any detectable...
Abstract Mutations in p53 are common human oral squamous cell carcinoma (OSCC). However, previous analyses, only detection of mutant protein using immunohistochemistry or mutations some exons have been examined. Full length many cases shows a loss tumor suppressor function, but possibly gain oncogenic function. In this study, we investigate relationships outcomes with the mutational spectrum (missense and truncation mutations) whole exon OSCC. Specimens from biopsy surgery (67 cases) were...
We isolated a metastasizing human esophageal squamous cell carcinoma (SCC) line, T.Tn‐AT1, from parental non‐metastasizing T.Tn, by in vitro selection and use of nude mouse orthotopic inoculation model. Then, we compared the expression profiles 9206 genes T.Tn‐AT1 T.Tn cDNA microarray analysis. The gene were very similar, only 34 showed more than 3‐fold differential expression. Among genes, 29 down‐regulated 5 up‐regulated cells. Subsequently, confirmed levels 14 cells means reverse...
Abstract The p53 gene is the most frequently mutated in malignant tumors. We found that abnormalities were observed cases of oral squamous cell carcinoma (OSCC). Therefore, it necessary to search for type functional abnormality (complete loss function, partial or gain oncogenic function) rather than presence absence mutation. Here we would like propose concept “p53 mutational spectrum”. In this study performed whole-exome sequencing using clinical specimens from OSCC and attempted clarify...
Background: Dental professionals were thought to have the most significant risk of coronavirus infection at pandemic period. Since first Corona-Virus Disease-2019 (COVID-19) patient was detected in Japan January 2020, has faced several waves Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) infections. However, no cluster SARS-CoV-2 infections associated with dental practice had been reported Japan. In this study, we aimed investigate actual status period through antibody testing...
BACKGROUND Neuroendocrine cell (NEC) carcinoma is occasionally accompanied by adenocarcinoma but the relationship between these two morphologically distinct tumours unclear. Two hypotheses have arisen regarding mechanism for association of and NEC carcinoma. One that both are derived from a common multipotential epithelial stem cell. The second hypothesis arise primitive NEC, respectively. AIMS To elucidate histologically tumours, stomach duodenum. PATIENT/METHODS We present case in which...
Tubular gland structures of colorectal cancer (CRC) have been demonstrated to undergo dedifferentiation at the primary site, and then are re‐formed in liver me‐tastases. In this study, we examined degree differentiation structure 48 cases CRCs (24 with synchronous metastasis, 24 without metastasis) by modified Gleason grading system. We also investigated role ZO‐1, one tight junction proteins, morphological changes, i.e., redifferentiation, site metastases. Liver‐metastasized (2.47±0.37)...
Abstract We established 5 rat bladder cell lines (MYU3L, MYU4, MYU6s, MYKUIL and MYP3). EGF stimulated DNA synthesis of all the cells in monolayer culture, regardless number receptors. In soft agar, only MYU3L formed colonies, enhanced their growth. However, did not induce other to grow agar. contrast, TGF‐β1 inhibited growth cells, but a tumorigenic which were from large vivo tumors more resistant than others TGF‐β1. tested effect factors on invasive potential MYP3 (non‐tumorigenic),...
We have previously reported that troglitazone inhibits proinflammatory cytokine production in chronic pancreatitis. In the present study, we show prevents progression of pancreatitis by inhibiting proliferation pancreatic stellate cells (PSCs) via a PPARgamma-independent mechanism. WBN/Kob rats with spontaneous were fed troglitazone-containing rat chow for 3 or 6 months. Pancreatic fibrosis and expression alpha-SMA markedly attenuated troglitazone. Rat PSCs expressed higher level PPARgamma1...
Oral squamous cell carcinoma (OSCC) histopathologically accounts for ≥90% of oral cancer. Many clinicopathological risk factors OSCC have also been proposed, and postoperative therapy is recommended in guidelines based on cancer stage other factors. However, even if the standard treatment provided according to guidelines, a few cases rapidly recur or show cervical distant metastasis. In this review article, we focus diversity origin OSCC. We discuss stem cells (CSCs) as key player explain...