A5100659142- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Bone fractures and treatments
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- MicroRNA in disease regulation
- Osteoarthritis Treatment and Mechanisms
- TGF-β signaling in diseases
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Silk-based biomaterials and applications
- Supramolecular Self-Assembly in Materials
- Proteoglycans and glycosaminoglycans research
- Hydrogels: synthesis, properties, applications
- Bone Metabolism and Diseases
- Cancer-related gene regulation
- Reconstructive Surgery and Microvascular Techniques
- Polymer Surface Interaction Studies
- Nuclear reactor physics and engineering
- Hedgehog Signaling Pathway Studies
- Orthopaedic implants and arthroplasty
University of California, Los Angeles
2016-2025
GS Caltex (South Korea)
2024-2025
Stanford University
2010-2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2024
Korea Forest Service
2014-2023
Atrium Health Wake Forest Baptist
2021-2023
Korea Polytechnic University
2021-2023
Sungkyunkwan University
2021
Takeda (Japan)
2021
Kangwon National University
2021
The major Food and Drug Association-approved osteoinductive factors in wide clinical use are bone morphogenetic proteins (BMPs). Although BMPs can promote robust formation, they also induce adverse effects, including cyst-like formation significant soft tissue swelling. In this study, we evaluated multiple BMP2 doses a rat femoral segmental defect model minimally traumatic onlay to determine its dose-dependent effects. Results of our established low concentration range (5 10 μg/mL, total...
Injectable hydrogels can fill irregular defects and promote in situ tissue regrowth regeneration. The ability of directing stem cell differentiation a three-dimensional microenvironment for bone regeneration remains challenge. In this study, we successfully nanoengineer an interconnected microporous networked photocrosslinkable chitosan situ-forming hydrogel by introducing two-dimensional nanoclay particles with intercalation chemistry. presence the nanosilicates increases Young's modulus...
Mesenchymal stem cell (MSC)-based transplantation is a promising therapeutic approach for bone regeneration and repair. In the realm of regeneration, defect or injured tissues are frequently inflamed with an abnormal expression inflammatory mediators. Growing evidence suggests that proinflammatory cytokines inhibit osteogenic differentiation formation. Thus, successful MSC-mediated repair, it important to overcome inflammation-mediated inhibition tissue regeneration. this study, using...
Background Human adipose-derived stromal cells (hASCs) represent a multipotent cell type with proven capacity to differentiate along an osteogenic lineage. This suggests that they may be used heal defects of the craniofacial or appendicular skeleton. We sought substantiate use undifferentiated hASCs in regeneration non-healing mouse skeletal defect. Methodology/Principal Findings ASCs were harvested from female lipoaspirate. Critical-sized (4 mm) calvarial created parietal bone adult male...
Unlike bone tissue, articular cartilage regeneration has not been very successful and many challenges ahead. We have previously developed injectable hydrogels using photopolymerizable chitosan (MeGC) that supported growth of chondrocytes. In this study, we demonstrate a biofunctional hydrogel for specific use in by conjugating transforming factor-β1 (TGF-β1), well-documented chondrogenic factor, to MeGC impregnating type II collagen (Col II), one the major cartilaginous extracellular matrix...
Administration of exosomes is considered an attractive cell-free approach to skeletal repair and pathological disease treatment. However, poor yield for the production technique unexpected therapeutic efficacy have been obstacles their widespread use in clinical practices. Here, we report alternative strategy produce exosome-related vesicles with high yields improved regenerative capability. An extrusion was employed amass exosome mimetics (EMs) from human mesenchymal stem cells (hMSCs). The...
Cartilaginous extracellular matrix (ECM) components such as type-II collagen (Col II) and chondroitin sulfate (CS) play a crucial role in chondrogenesis. However, direct clinical use of natural Col II or CS scaffolds for cartilage tissue engineering is limited by their instability rapid enzymatic degradation. Here, we investigate the incorporation into injectable chitosan hydrogels designed to gel upon initiation exposure visible blue light (VBL) presence riboflavin. Unmodified hydrogel...
Clinically available sources of bone for repair and reconstruction are limited by the accessibility autologous grafts, infectious risks cadaveric materials, durability synthetic substitutes. Cell-based approaches skeletal regeneration can potentially fill this need, adipose tissue represents a promising source development such therapies. Here, we enriched an osteogenic subpopulation cells derived from human subcutaneous utilizing microfluidic-based single cell transcriptional analysis...
Three-dimensional printing (3DP) is a rapid prototyping technique that can create complex 3D structures by inkjet of liquid binder onto powder biomaterials for tissue engineering scaffolds. Direct fabrication scaffolds from 3DP, however, imposes limitation on material choices manufacturing processes. In this study, we report an indirect 3DP approach wherein positive replica desired shapes was printed using gelatin particles, and the final scaffold directly produced mold. To patient-specific...
Photopolymerizable hydrogels derived from naturally occurring polymers have attracted significant interest in tissue-engineering applications due to their excellent biocompatibility, hydrophilic nature favourable for cell ingrowth and ability be cured situ through a minimally invasive procedure. In this study, we developed composite hydrogel consisting of photocrosslinkable methacrylated glycol chitosan (MeGC) semi-interpenetrating collagen (Col) with riboflavin photoinitiator under blue...
Pluripotent cells represent a powerful tool for tissue regeneration, but their clinical utility is limited by propensity to form teratomas. Little known about interaction with the surrounding niche following implantation and how this may be applied promote survival functional engraftment. In study, we evaluated ability of an osteogenic microniche consisting hydroxyapatite-coated, bone morphogenetic protein-2–releasing poly- l -lactic acid scaffold placed within context macroenvironmental...
Bone repair is a complex process involving the sophisticated interplay of osteogenic stem cells, extracellular matrix, and osteoinductive factors, it affected by bacterial toxins oxidative stress. Inspired nature plant-derived phytochemicals inorganic-organic analogues bone we report herein facile design nanoclay-organic hydrogel sealant (NoBS) that integrates multiple physico-chemical cues for regeneration into single system. Assembly phytochemical-modified organic chitosan silica-rich...
Tuning hydrogel degradation enables effective and successful tissue regeneration by modulating cellular behaviors matrix formation. In this work, we develop a novel degradable scaffold on the basis of unique enzyme-substrate complex photocrosslinking. Chitosan lysozyme are chemically modified with methacrylate moieties to be tethered in hydrogels, presence riboflavin initiator, these hydrogels cured blue light irradiation. The incorporation chitosan accelerates rate crosslinked...
Epigenetic mechanisms are fundamental to understanding the regulatory networks of gene expression that govern stem cell maintenance and differentiation. Methylated histone H3 lysine 4 (H3K4) has emerged as a key epigenetic signal for transcription; it is dynamically modulated by several specific H3K4 methyltransferases demethylases. Recent studies have described new which methylation modifiers control self-renewal lineage commitments cells. Such advances in biology would high impact on...
Extracellular vesicles have received a great interest as safe biocarriers in biomedical engineering. There is need to develop more efficient delivery strategies improve localized therapeutic efficacy and minimize off-target adverse effects. Here, exosome mimetics (EMs) are reported for bone targeting involving the introduction of hydroxyapatite-binding moieties through bioorthogonal functionalization. Bone-binding ability engineered EMs verified with hydroxyapatite-coated scaffolds an ex...
Demineralized bone matrix (DBM) has been widely used as an allogeneic alternative to autologous graft for repair. However, more extensive use of DBM is limited due its particulate nature after demineralization and rapid particle dispersion following irrigation, resulting in unpredictable osteoinductivity. Here, a new design injectable hydrogel carriers that combine self-healing ability osteogenic properties based on the self-assembly guanidinylated hyaluronic acid silica-rich nanoclays...
Developing novel strategies for defeating osteoporosis has become a world-wide challenge with the aging of population. In this work, supramolecular nanoagonists (NAs), constructed from alkaloids and phenolic acids, emerge as carrier-free nanotherapy efficacious treatment. These precision are formed through self-assembly berberine (BER) chlorogenic acid (CGA), utilizing noncovalent electrostatic, π–π, hydrophobic interactions. This assembly results in 100% drug loading capacity stable...