A5101910726- Advanced Proteomics Techniques and Applications
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Osteoarthritis Treatment and Mechanisms
- Organ Transplantation Techniques and Outcomes
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Mass Spectrometry Techniques and Applications
- Liver Disease and Transplantation
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Mitochondrial Function and Pathology
- Liver physiology and pathology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver Disease Diagnosis and Treatment
- Mesenchymal stem cell research
- Machine Learning in Bioinformatics
Dalian Medical University
2016-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Tianjin University of Traditional Chinese Medicine
2025
Second Affiliated Hospital of Dalian Medical University
2015-2024
Rutgers, The State University of New Jersey
2022-2024
Qilu Hospital of Shandong University
2024
Nanjing Medical University
2013-2024
Army Medical University
2017-2023
Daping Hospital
2017-2023
Guilin Medical University
2023
Abstract Recent interest in the control of bone metabolism has focused on a specialized subset CD31 hi endomucin vessels, which are reported to couple angiogenesis with osteogenesis. However, underlying mechanisms that link these processes together remain largely undefined. Here we show zinc-finger transcription factor ZEB1 is predominantly expressed endothelium human and mouse bone. Endothelial cell-specific deletion mice impairs vessel formation bone, resulting reduced Mechanistically,...
Long non-coding RNAs (lncRNAs) are key players in the development and progression of human cancers. The lncRNA XIST (X-inactive specific transcript) has been shown to be upregulated non-small cell lung cancer (NSCLC); however, its role molecular mechanisms NSCLC remain unclear.qRT-PCR was conducted assess expression miR-186. Cell proliferation detected using MTT assay. invasion migration were evaluated transwell cycle distribution apoptosis rates analyzed by flow cytometry. Luciferase...
Abstract Treatment of osteochondral defects remains a great challenge in clinical practice because cartilage and subchondral bone possess significantly different physiological properties. In this study, the controlled surface micro/nanometer structure bioactive scaffolds combination biomaterial chemistry is harnessed to address issue. Model biomaterials, bredigite (BRT) scaffolds, with micro/nanostructure are successfully fabricated by combining 3D printing hydrothermal process. It found...
Chromobox 2 (CBX2), a chromobox family protein, is crucial component of the polycomb group complex: repressive complex 1 (PRC1). Research on CBX2 as an oncogene has been published in recent years. However, connection between and hepatocellular carcinoma (HCC) not studied. In this article, based results immunohistochemical (IHC) staining HCC adjacent liver tissue microarrays, we found that high expression associated with poor prognosis patients. The CCK8 assay, clonogenic survival assay nude...
Abstract Alpha-enolase (ENO1) has been found to be dysregulated in several human malignancies, including hepatocellular carcinoma (HCC). Although the role of ENO1 as a glycolytic enzyme HCC cells well characterized, little is known about other roles ENO1, especially exosome-derived regulating progression. Here, we demonstrated that frequently upregulated or tissues, with even higher expression highly metastatic tissues exosomes derived from sources. Moreover, associated tumor-node-metastasis...
Background and Aim Hepatocellular carcinoma (HCC) is one of the most deadly tumors. Transarterial chemoembolization (TACE) effective for unresectable HCC. In recent years, miRNAs have been proposed as novel diagnostic prognostic tools This study aimed to identify whether microRNAs (miRNAs) can serve biomarkers reliably predict outcome before HCC patients are treated with TACE. Methods Eleven (miR-, miR-19a, miR-101-3p, miR-199a-5p, miR-200a, miR-21, miR-214, miR-221, miR-222, miR-223 miR-,...
Tubulointerstitial inflammation plays a critical role in the progression of diabetic nephropathy (DN), and nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasomes contribute to renal interstitial DN. Decreased expression optineurin (OPTN) is also associated with We investigated OPTN activation NLRP3 inflammasome initially examined biopsy tissues 172 patients type 2 DN 32 nondiabetic hamartoma. Expression was significantly lower...
Although glycoproteomics is greatly developed in recent years, our knowledge about N-glycoproteome of human tissues still very limited. In this study, we comprehensively mapped the N-glycosylation sites liver by combining click maltose-hydrophilic interaction chromatography (HILIC) and improved hydrazide chemistry. The specificity could be as high 90% for chemistry 80% HILIC. Altogether, identified 14,480 N-glycopeptides matched with N-!P-[S|T|C] sequence motif from liver, corresponding to...
Abstract Background The use of stem cell-derived exosomes (Exos) as therapeutic vehicles is receiving increasing attention. Exosome administration has several advantages over cell transplantation, thus making promising candidates for large-scale clinical implementation and commercialization. However, exosome extraction purification efficiencies are relatively low, heterogeneity high due to differences in culture conditions viability. Therefore, this study, we investigated a priming procedure...
To investigate the role of post-operative intensity-modulated radiotherapy (IMRT) in patients receiving narrow-margin hepatectomy for hepatocellular carcinoma (HCC) located close to major vessels.This exploratory study involved 181 HCC patients. Of them, 116 were treated with (<1.0 cm) hepatectomy. Thirty-three underwent postoperative IMRT (Group A), while 83 did not receive B). The remaining 65 wide-margin (≥1.0 C). Prognosis and patterns recurrence assessed three groups.The 3-year overall...
// Tadashi Ogawa 1, 2 , Yoshihiko Hirohashi 1 Aiko Murai Toshihiko Nishidate Kenji Okita Liming Wang Yuzuru Ikehara 3 Tetsuta Satoyoshi Akihiro Usui Terufumi Kubo Munehide Nakastugawa Takayuki Kanaseki Tomohide Tsukahara Goro Kutomi Tomohisa Furuhata Koichi Hirata Noriyuki Sato Toru Mizuguchi Ichiro Takemasa and Torigoe Department of Pathology, Sapporo Medical University School Medicine, Chuo-Ku, 060-8556, Japan Surgery, The Molecular Medicine Team, Research Center for Glycoscience, National...
// Yu Tian 1 , Bo Tang 2 Chengye Wang Deguang Sun Rixin Zhang Nan Luo 3 Zhao Han Rui Liang Zhenming Gao Liming Division of Hepatobiliary and Pancreatic Surgery, Department The Second Affiliated Hospital Dalian Medical University, Dalian, Liaoning, P.R. China Guilin Guilin, Guangxi, Infection, Correspondence to: Wang, email: wangbcc259@163.com Tang, dytangbo@163.com Keywords: metformin, hepatocellular carcinoma, 5-fluorouracil, YAP Received: March 30, 2016 Accepted: May 29, Published: June...
SIRT5 belongs to a family of NAD+-dependent lysine deacetylases called sirtuins. Although accumulating evidence indicates upregulation in cancers, including liver cancer, the detailed roles and mechanisms remain be revealed. Hepatocellular carcinoma (HCC) is second leading cause cancer-related deaths among men worldwide, finding effective targets for HCC treatment prevention urgently needed. In present study, we confirmed that mitochondrial sirtuins, particularly SIRT5, are more highly...
Mesenchymal stem cells (MSCs) have tumor-homing ability and play critical roles in tumor treatment, but their dual influences on progression limit therapeutic applications. Exosomes derived from MSCs (MSC-exosomes) exhibit great potential targeted treatment due to advantages of high stability, low immunogenicity, good biocompatibility, long circulation time homing characteristics. Furthermore, the artificial modification MSC-exosomes could amplify inhibitory effect tumors overcome...
Articular cartilage injury impairs joint function and necessitates orthopedic intervention to restore the structure of cartilage. Extracellular matrix (ECM) scaffolds derived from bone marrow mesenchymal stem cells (BMSCs) can effectively promote cell adhesion, proliferation, chondrogenesis. However, pre-shaped ECM have limited applicability due their poor fit with irregular surface most articular defects. In this study, we fabricated an injectable active hydrogel autologous BMSCs-derived by...
Purpose. To examine the inhibitory effects of star fruit (Averrhoa carambola) juice towards seven major cytochrome P450 (CYP) isoforms and NADPH-cytochrome reductase (CPR). Methods. The (0.5 to 5%, v/v) against activities CYP including CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP3A4 CPR were examined in human liver microsomes. identify time-dependent inhibition, (2.5%, was preincubated with microsomes a NADPH-generating system for 0-15 min, then extent inhibition examined. Results....
Reversible protein phosphorylation plays a critical role in liver development and function. Comprehensively cataloging the phosphoproteins their sites human tissue will facilitate understanding of physiological pathological mechanisms liver. Owing to lacking efficient approach fractionate phosphopeptides, nanoflow-RPLC with long-gradient elution was applied reduce complexity phosphopeptides this study. Two approaches were performed further improve coverage phosphoproteome analysis tissue. In...