A5013959327- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Cardiac Ischemia and Reperfusion
- 3D Printing in Biomedical Research
- Erythropoietin and Anemia Treatment
- Signaling Pathways in Disease
- Coronary Interventions and Diagnostics
- Ultrasound and Hyperthermia Applications
- Cardiovascular Function and Risk Factors
- Cardiac pacing and defibrillation studies
- Cardiovascular Disease and Adiposity
- Cardiac electrophysiology and arrhythmias
- Transplantation: Methods and Outcomes
- Immune cells in cancer
- Laser Applications in Dentistry and Medicine
- Adipose Tissue and Metabolism
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- IL-33, ST2, and ILC Pathways
- Cardiac Imaging and Diagnostics
- Laser Material Processing Techniques
- Viral Infections and Immunology Research
- Cardiac Valve Diseases and Treatments
University of Rostock
2013-2023
Ludwig-Maximilians-Universität München
2013
Deutsches Herzzentrum der Charité
2013
University Hospital Cologne
2013
Tissue Dynamics (Israel)
2013
Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem (hMSC) were chosen laser experiments due to their high potential regeneration application possibilities therapy. To evaluate influence LIFT cells, survival rate, proliferation apoptotic activity, DNA damages modifications...
The possible different therapeutic efficacy of human mesenchymal stem cells (hMSC) derived from umbilical cord blood (CB), adipose tissue (AT) or bone marrow (BM) for the treatment myocardial infarction (MI) remains unexplored. This study was to assess regenerative potential hMSC origins and evaluate role CD105 in cardiac regeneration. Male SCID mice underwent LAD-ligation received respective cell type (400.000/per animal) intramyocardially. Six weeks post infarction, catheterization showed...
Human Mesenchymal Stem Cells (hMSCs) present a promising tool for regenerative medicine. However, ex vivo expansion is necessary to obtain sufficient cells clinical therapy. Conventional growth media usually contain the critical component fetal bovine serum. For use, chemically defined will be required. In this study, capability of two commercial, defined, serum-free hMSC (MSCGM-CD and PowerStem) proliferation was examined compared serum-containing medium (MSCGM). Immunophenotyping hMSCs...
Abstract Myocardial infarction ( MI ) is a major condition causing heart failure HF ). After , the renin angiotensin system RAS and its signalling octapeptide II (Ang II) interferes with cardiac injury/repair via AT 1 2 receptors 1R, 2R). Our study aimed at deciphering mechanisms underlying link between cellular components of immune response relying on rodent model as well patients. Flow cytometric analyses showed an increase in expression CD 4 + 2R cells rat spleen post‐infarction, but...
BackgroundBone marrow stem cell clonal dysfunction by somatic mutation is suspected to affect post-infarction myocardial regeneration after coronary bypass surgery (CABG).MethodsTranscriptome and variant expression analysis was studied in the phase 3 PERFECT trial post infarction CABG CD133+ bone derived hematopoetic cells showing difference left ventricular ejection fraction (∆LVEF) Responders (n=14; ∆LVEF +16% day 180/0) Non-responders (n=9; -1.1% 180/0). Subsequently, findings have been...
Bone marrow (BM)-derived stem cells with their various functions and characteristics have become a well-recognized source for the cell-based therapies. However, knowledge on therapeutic potential shortage cross-link between distinct BM-derived cells, primed after onset of myocardial infarction (MI), seems to be still rudimentary. Therefore, post-examination such hematopoietic CD133+ mesenchymal CD271+ was object present study.The effects respective mononuclear alone as well in co-culture...
Abstract Different subtypes of bone marrow-derived stem cells are characterized by varying functionality and activity after transplantation into the infarcted heart. Improvement cell therapeutics requires deep knowledge about mechanisms that mediate benefits treatment. Here, we demonstrated co-transplantation mesenchymal (MSCs) hematopoietic (HSCs) led to enhanced synergistic effects on cardiac remodeling. While HSCs were associated with blood vessel formation, MSCs found possess...
Ischemic heart failure still displays the highest mortality. An early boost of intracardiac regenerative key mechanisms and angiogenetic niche signaling in cardiac mesenchymal stem cells (MSCs) could improve myocardial infarction (MI) healing. Epicardial erythropoietin (EPO, 300U kg−1) was compared with intraperitoneal intramyocardial EPO treatments after acute MI rats (n=156). Real-time PCR confocal microscopy revealed epicardial treatment enhanced indicators (SDF-1, CXCR-4, CD34, Bcl-2,...
Cellular inflammation following acute myocardial infarction has gained increasing importance as a target mechanism for therapeutic approaches. We sought to investigate the effect of syngeneic cardiac induced cells (CiC) on using 18F-FDG PET (Positron emission tomography)-based imaging and resulting pump function magnetic resonance (CMR) in mouse model infarction. Mice underwent permanent left anterior descending coronary artery (LAD) ligation inducing an inflammatory response. The therapy...
CD133+ stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. Several clinical trials have shown remarkable beneficial effects following their intramyocardial transplantation. Yet, the purification of is typically performed centralized clean room facilities using semi-automatic manufacturing processes based on magnetic cell sorting (MACS®). However, this requires time-consuming and cost-intensive logistics.CD133+ were purified from...
The immune response is a crucial factor for mediating the benefit of cardiac cell therapies. Our previous research showed that cardiomyocyte transplantation alters and, when combined with short-term pharmacological CCR2 inhibition, resulted in diminished functional benefit. However, specific role innate cells, especially macrophages on outcome transplantation, unclear. We compared cellular, molecular, and following wildtype T cell- B cell-deficient Rag2del mice. inflammatory was assessed...
Cardiac mesenchymal stem cells (MSCs) could stimulate cell-specific regenerative mechanisms after myocardial infarction (MI) depending on spatial origin, distribution, and niche regulation. We aimed at identifying isolating tissue-specific cardiac MSCs that contribute to regeneration.Following permanent ligation of the left anterior descending coronary artery in rats (n = 16), early tissues mononuclear (MNCs) were analyzed by immunohistology, confocal laser scanning microscopy, flow...
Background: Ventricular arrhythmias (VA) are a common cause of sudden death after myocardial infarction (MI). Therefore, developing new therapeutic methods for the prevention and treatment VA is prime importance. Methods: Human bone marrow derived CD271+ mesenchymal stem cells (MSC) were tested their antiarrhythmic effect. This was done through development novel mouse model using an immunocompromised Rag2−/− γc−/− strain subjected to “infarction-reinfarction”. The mice underwent first...
We investigated the influence of syngeneic cardiomyocyte transplantation after myocardial infarction (MI) on immune response and cardiac function. Methods Results: show for first time that is altered as a result neonatal MI leading to improved pump function observed by magnetic resonance imaging in C57BL/6J mice. Interestingly, there was no improvement capillary density well infarct area CD31 Sirius Red staining, respectively. Flow cytometric analysis revealed significantly different...
In-depth knowledge of the mechanisms induced by early postischemic cardiac endogenous mesenchymal stem cells (MSCs) in acutely ischemic heart could advance our understanding regeneration. Herein, we aimed to identify, isolate, and initially characterize origin, kinetics fate MSCs. This was facilitated vivo genetic cell mapping through green fluorescent protein (GFP) expression under control vimentin induction after acute myocardial infarction (MI). Following permanent ligation left anterior...
The data presented in this article are related to the research "Intramyocardial Fate and Effect of Iron Nanoparticles co-injected with MACS® purified Stem Cell Products" (Müller et al., 2017) [1]. This complements cellular localization superparamagnetic iron dextran particles (MACS® MicroBeads) used for magnetic activated cell sorting (MACS®). Data evaluate time-dependent detachment these nanoparticles from CD133+ haematopoietic stem cells (HSCs) CD271+ mesenchymal (MSCs). Furthermore,...
CD117(+) stem cell (SC) based therapy is considered an alternative therapeutic option for terminal heart disease. However, controversies exist on the effects of SC implantation. In particular, link between function and angiotensin-II-type-2 receptor (AT2R) after MI continuously discussed. We therefore asked whether 1) AT2R stimulation influences properties in vitro and, 2) which can be ascribed to vivo.We approached with Angiotensin II while simultaneously blocking its opponent AT1 Losartan....
Several cell populations derived from bone marrow (BM) have been shown to possess cardiac regenerative potential. Among these are freshly isolated CD133+ hematopoietic as well culture-expanded mesenchymal stem cells. Alternatively, by purifying CD271+ cells BM, progenitors can be enriched without an ex vivo cultivation. With regard the limited available number of BM-derived cells, effect dosage on therapeutic efficiency is particular interest. Therefore, in present pre-clinical study, we...
Ventricular arrhythmias associated with myocardial infarction (MI) have a significant impact on mortality in patients following heart attack. Therefore, targeted reduction of arrhythmia represents therapeutic approach for the prevention and treatment severe events after infarction. Recent research transplanting mesenchymal stem cells (MSC) showed their potential MI therapy. Our study aimed to investigate effects MSC injection post-infarction arrhythmia. We used our murine double model, which...
Objectives: Local sustained erythropoietin (EPO) release from epicardium might improve the therapy of myocardial infarction (MI) through an early boost intracardiac key mechanisms as well accelerated proliferation and tissue transformation in mesenchyme.
Abstract Optimizing endothelialization of medical implants requires deep mechanistic insight into cellular adhesion, cell junction and physiological basement membrane development at the endothelial cell-to-scaffold substrate interface. We employed standardized cells fibrin hydrogel for simultaneous cell-plus-fibrin (EC-Fib) spray application using Maslanka Direct sprayed outlined necessity preconditioning acellular SynerGraft