A5062415727- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Cardiac Valve Diseases and Treatments
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Bone Tissue Engineering Materials
- Nanoparticle-Based Drug Delivery
- Infective Endocarditis Diagnosis and Management
- Aortic Disease and Treatment Approaches
- Coronary Interventions and Diagnostics
- Advanced Polymer Synthesis and Characterization
- biodegradable polymer synthesis and properties
- Nanofabrication and Lithography Techniques
- Connective tissue disorders research
- Angiogenesis and VEGF in Cancer
- Cardiac pacing and defibrillation studies
- Elasticity and Material Modeling
- Mesenchymal stem cell research
- Characterization and Applications of Magnetic Nanoparticles
- Colorectal Cancer Surgical Treatments
- Cardiac Structural Anomalies and Repair
- Block Copolymer Self-Assembly
- Cardiac and Coronary Surgery Techniques
- Advanced Sensor and Energy Harvesting Materials
- Polymer Surface Interaction Studies
Technical University of Munich
2019-2024
Institute for Biomedical Engineering
2019-2024
RWTH Aachen University
2014-2023
Implant Sciences (United States)
2021
Max Planck Computing and Data Facility
2021
Maastricht University
2018
VIB-KU Leuven Center for Cancer Biology
2018
Helmholtz Institute Mainz
2016
FH Aachen
2016
Universitätsklinikum Aachen
2012
Abstract Heart valves are characterized to be highly flexible yet tough, and exhibit complex deformation characteristics such as nonlinearity, anisotropy, viscoelasticity, which are, at best, only partially recapitulated in scaffolds for heart valve tissue engineering (HVTE). These biomechanical features dictated by the structural properties microarchitecture of major constituents, particular collagen fibers. In this study, unique capabilities melt electrowriting (MEW) exploited create...
Abstract Heart valve tissue engineering (HVTE) aims to provide living autologous heart implants endowed with regenerative capabilities and life‐long durability. However, fabrication of biomimetic scaffolds capable providing the required functionality in terms mechanical performance tunable porosity enable cellular infiltration remains a major challenge. Here, additive manufacturing bioinspired, spatially heterogeneous, tubular enclosing leaflets, inter‐leaflet triangles, their interface for...
The manufacture of fibrous scaffolds with tailored micrometric features and anatomically relevant three-dimensional (3D) geometries for soft tissue engineering applications remains a great challenge. Melt electrowriting (MEW) is an advanced additive manufacturing technique capable depositing predefined fibers. However, it has been so far inherently limited to simple planar tubular scaffold because the need avoid polymer jet instabilities. In this work, we surmount technical boundaries MEW...
Vascular disease is a leading cause of death worldwide, but surgical options are restricted by the limited availability autologous vessels, and suboptimal performance prosthetic vascular grafts. This especially evident for coronary artery by-pass grafts, whose small caliber associated with high occlusion propensity. Despite potential tissue-engineered compliance mismatch, dilatation, thrombus formation, lack functional elastin still major limitations to graft failure. calls advanced...
Background Preoperative anxiety in cardiac surgery can lead to prolonged hospital stays and negative postoperative outcomes. An improved patient education using 3D models may reduce preoperative risks associated with it. Methods Patient was performed standardized paper-based methods ( n = 34), 3D-printed 34) or virtual reality 31). Anxiety procedural understanding were evaluated questionnaires prior after the education. Additionally, time spent for overall quality among further basic...
The general approach in heart valve tissue engineering is to mimic the shape of native attempt recreate natural haemodynamics. In this article, we report fabrication first tissue-engineered (TEHV) based on a tubular leaflet design, where function leaflets semilunar valves performed by simple construct sutured along circumferential line at root and three single points sinotubular junction. design recent development pericardial (nonviable) bioprostheses, which has attracted interest because...
Transcatheter aortic valve implantation of (nonviable) bioprosthetic valves has been proven a valid alternative to conventional surgical in patients at high or prohibitive mortality risk. In this study we present the vitro proof-of-principle newly developed tissue-engineered heart for minimally invasive implantation, with ultimate aim adding unique advantages living tissue regeneration capabilities continuously developing transcatheter technologies. The tube-in-stent is fibrin-based tubular...
This study is focussed on the optimal method of autologous fibrinogen isolation with regard to yield and use as a scaffold material. particularly relevant for pediatric patients strictly limited volumes blood.The following methods were evaluated: cryoprecipitation, ethanol (EtOH) precipitation, ammonium sulfate [(NH(4))(2)SO(4))] precipitation combined polyethylene glycol cryoprecipitation. Fibrinogen yields quantified spectrophotometrically by electrophoretic analyses. To test influence...
The mechanical properties of tissue‐engineered heart valves still need to be improved enable their implantation in the systemic circulation. aim this study is develop a valve for aortic position – BioTexValve by exploiting bio‐inspired composite textile scaffold confer native‐like strength and anisotropy leaflets. This achieved multifilament fibers arranged similarly collagen bundles native leaflet, fixed thin electrospun layer directly deposited on pattern. textile‐based leaflets are...
Studies in which mesenchymal stromal cells (MSC) from the placenta are compared with multiple MSC types other sources rare. The chorionic plate of human is mainly composed fetal blood vessels embedded stroma tissue, lined by trophoblastic and organized into villi (CV) structures. We comprehensively characterized collected postnatal (CV-MSC) analyzing their growth proliferation potential, differentiation, immunophenotype, extracellular matrix production, telomere length, aging phenotype,...
Iron oxide nanoparticles (IONs) are of great interest in nanomedicine for imaging, drug delivery, or hyperthermia treatment. Although many research groups have focused on the synthesis and application IONs nanomedicine, little is known about influence surface properties particles' behavior human body. This study analyzes impact coatings (dextran, polyvinyl alcohol, polylactide-co-glycolide) nanoparticles' cytocompatibility, agglomeration, degradation, resulting oxidative stress induced by...
Abstract Scaffolds with multiple advantageous biological and structural properties are still a challenge in the field of tissue engineering. The convergence advanced fabrication techniques functional materials is key to fulfill this need. Melt electrowriting (MEW) an additive manufacturing technique that enables microfibrous scaffolds precisely defined microarchitectures. Here, it proposed exploit metal–organic frameworks (MOFs) efficiently introduce multifunctionalities by combining...
In the field of tissue engineering, there is a growing need for quantitative methods to analyze in situ and real time development three-dimensional scaffolds. To evaluate performance cell-gel constructs terms extracellular matrix synthesis, we are still restricted time-consuming histological biochemical assays that not able, as destructive method, monitor formation online. Ultrasound well-known noninvasive nondestructive imaging method. Therefore, potential ultrasound vitro evaluation...
Heart valves are elaborate and highly heterogeneous structures of the circulatory system. Despite well accepted relationship between structural mechanical anisotropy optimal function valves, most approaches to create tissue-engineered heart (TEHVs) do not try mimic this complexity rely on one homogenous combination cells materials for whole construct. The aim study was establish an easy versatile method introduce spatial diversity into a valve fibrin scaffold. We developed multiple-step...
Vascular grafts are used to bypass damaged or diseased blood vessels. Bacterial cellulose (BC) has been studied for use as an off‐the‐shelf graft. Herein, we present a novel, cost‐effective, method the production of small caliber BC with minimal processing requirements. The morphology graft wall produced tensile strength above that native vessels, performing similarly current commercial alternatives. As result method, luminal surface presents similar topography We have also in vivo behavior...
Carboxymethyl-dextran (CMD)-coated iron oxide nanoparticles (IONs) are of great interest in nanomedicine, especially for applications drug delivery. To develop a magnetically controlled delivery system, many factors must be considered, including the composition, surface properties, size and agglomeration, magnetization, cytocompatibility, activity. This study reveals how CMD coating thickness can influence these particle properties. ION@CMD synthesized by co-precipitation. A higher quantity...
Abstract Melt electrowriting (MEW) enables the electric field‐assisted digital fabrication of precisely defined scaffold architectures micron‐sized fibers. However, charge accumulation and consequent disruption precoded pattern by fiber bridging prevents controlled printing at small interfiber distances. This, together with periodical layer stacking characteristic for additive manufacturing, typically results in scaffolds channel‐like macroporosity, which need to be combined other...
Small diameter vascular grafts (SDVGs) are associated with a high failure rate due to poor endothelialization. The incorporation of nitric oxide (NO) releasing system improves biocompatibility by using the NO effect promote endothelial cell (EC) migration and proliferation while preventing bacterial infection. To circumvent instability donors prolong releasing, S-nitroso-N-acetyl-D-penicillamine (SNAP) as donor was loaded in multi-walled carbon nanotubes (MWCNTs). Successful loading...
Coated iron oxide nanoparticles (IONs) are promising candidates for various applications in nanomedicine, including imaging, magnetic hyperthermia, and drug delivery. The application of IONs nanomedicine is influenced by factors such as biocompatibility, surface properties, agglomeration, degradation behavior, thrombogenicity. Therefore, it essential to investigate the effects coating material thickness on behavior performance human body. In this study, with a carboxymethyl dextran (CMD) two...