A5102926205- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Wireless Power Transfer Systems
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Cardiac and Coronary Surgery Techniques
- Microfluidic and Bio-sensing Technologies
University of Rostock
2013-2017
University Medical Center
2016
Bone marrow derived human mesenchymal stem cells (hMSCs) show promising potential in regeneration of defective tissue. Recently, gene silencing strategies using microRNAs (miR) emerged with the aim to expand therapeutic hMSCs. However, researchers are still searching for effective miR delivery methods clinical applications. Therefore, we aimed develop a technique efficiently deliver into hMSCs help magnetic non-viral vector based on cationic polymer polyethylenimine (PEI) bound iron oxide...
Magnetically guided transfection has been shown as a promising approach for the genetic modification of cells. We observed that polyethylenimine (PEI)-condensed pDNA, combined with magnetic nanoparticles (MNPs) via biotin-streptavidin interactions could provide higher efficiency than pDNA/PEI alone, even without application force. Therefore, we intended to investigate beneficial properties MNP-based transfection.We performed three-color fluorescent labeling complexes and traced them inside...
Genetic modifications of bone marrow derived human mesenchymal stem cells (hMSCs) using microRNAs (miRs) may be used to improve their therapeutic potential and enable innovative strategies in tissue regeneration. However, most the studies use cultured hMSCs, although these can lose cell characteristics during expansion. Therefore, we aimed develop a nonviral miR carrier based on polyethylenimine (PEI) bound magnetic nanoparticles (MNPs) for efficient delivery freshly isolated hMSCs. MNP...
Abstract Different subtypes of bone marrow-derived stem cells are characterized by varying functionality and activity after transplantation into the infarcted heart. Improvement cell therapeutics requires deep knowledge about mechanisms that mediate benefits treatment. Here, we demonstrated co-transplantation mesenchymal (MSCs) hematopoietic (HSCs) led to enhanced synergistic effects on cardiac remodeling. While HSCs were associated with blood vessel formation, MSCs found possess...
CD133+ stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. Several clinical trials have shown remarkable beneficial effects following their intramyocardial transplantation. Yet, the purification of is typically performed centralized clean room facilities using semi-automatic manufacturing processes based on magnetic cell sorting (MACS®). However, this requires time-consuming and cost-intensive logistics.CD133+ were purified from...
Aim . CD133 + stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended develop a nonviral system appropriate engineering. Materials Methods Modification of human with magnetic polyplexes carrying microRNA was studied terms efficiency, safety, targeting potential. Results High uptake rates (~80–90%) were achieved without affecting properties. Modified...
Human bone marrow stem cell populations have been applied for cardiac regeneration purposes within different clinical settings in the recent past. The migratory capacity of towards injured tissue, after undergoing specific peri-interventional harvesting and isolation procedures, represents a key factor limiting therapeutic efficacy. We therefore aimed at analyzing human cluster differentiation (CD) 133(+) cells vivo intraoperative from sternal marrow. CD133(+) were isolated patients surgery...