A5027810417- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Cardiac Fibrosis and Remodeling
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Business and Management Studies
- Extracellular vesicles in disease
- Cardiac and Coronary Surgery Techniques
- Cardiomyopathy and Myosin Studies
- RNA modifications and cancer
- Wireless Power Transfer Systems
- CRISPR and Genetic Engineering
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Neuroscience and Neural Engineering
- Trauma and Emergency Care Studies
- Cancer-related molecular mechanisms research
- Osteoarthritis Treatment and Mechanisms
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
Bundeswehrzentralkrankenhaus Koblenz
2024
Bundeswehr
2024
University of Rostock
2014-2020
University Medical Center
2016-2018
Universidade de Caxias do Sul
2017
Karolinska Institutet
2014
The phase III clinical trial PERFECT was designed to assess safety and efficacy of intramyocardial CD133+ bone marrow stem cell treatment combined with CABG for induction cardiac repair.Multicentre, double-blinded, randomised placebo controlled trial.The study conducted across six centres in Germany October 2009 through March 2016 stopped due slow recruitment after positive interim analysis 2015.Post-infarction patients chronic ischemia reduced LVEF (25-50%).Eighty-two were two groups...
Analyses on the cellular level are indispensable to expand our understanding of complex tissues like mammalian heart. Single-nucleus sequencing (snRNA-seq) allows for exploration composition and cell features without major hurdles single-cell sequencing. We used snRNA-seq investigate first time an entire adult quantification clustering led accurate representation types, revealing 24 distinct clusters with endothelial cells (28.8%), fibroblasts (25.3%), cardiomyocytes (22.8%) constituting...
In umbilical cord blood (UCB) transplantation, the number of nucleated cells per kilogram is a major predictive and critical factor hematopoietic recovery. Thus, ex vivo expansion UCB progenitors could potentially accelerate engraftment. Whereas Flt-3 ligand (FL), stem cell (SCF), thrombopoietin (TPO) are considered indispensable, role interleukin 3 (IL-3) still controversial: it has been reported either to support or abrogate reconstituting ability cells. By adding IL-3 we aimed enhance...
The recent development and broad application of sequencing techniques at the single-cell level is generating an unprecedented amount data. different have their individual limits, but datasets also offer unexpected possibilities when utilized collectively. Here, we applied snRNA-seq in whole adult murine hearts from inbred (C57BL/6NRj) outbred (Fzt:DU) mouse strain to directly compare data with publicly available scRNA-seq tabula muris project. Explicitly choosing a single-nucleus approach...
Genetic modifications of bone marrow derived human mesenchymal stem cells (hMSCs) using microRNAs (miRs) may be used to improve their therapeutic potential and enable innovative strategies in tissue regeneration. However, most the studies use cultured hMSCs, although these can lose cell characteristics during expansion. Therefore, we aimed develop a nonviral miR carrier based on polyethylenimine (PEI) bound magnetic nanoparticles (MNPs) for efficient delivery freshly isolated hMSCs. MNP...
CD133+ stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. Several clinical trials have shown remarkable beneficial effects following their intramyocardial transplantation. Yet, the purification of is typically performed centralized clean room facilities using semi-automatic manufacturing processes based on magnetic cell sorting (MACS®). However, this requires time-consuming and cost-intensive logistics.CD133+ were purified from...
Abstract Despite significant progress in the medical field, there is still a pressing need for minimal‐invasive tools to assist with decision‐making, especially cases of polytrauma. Our team explored potential serum‐derived large extracellular vesicles, so called microparticles/microvesicles/ectosomes, serve as supportive tool decision‐making polytrauma situations. We focused on whether monocyte derived EVs may differentiate between patients internal organ injury (ISS > 15) and those...
By far, most strategies for cell reprogramming and gene therapy are based on the introduction of DNA after viral delivery. To avoid high risks accompanying these goals, non-viral DNA-free delivery methods various types required.Relying an initially established PCR-based protocol convenient template production, we synthesized five differently modified EGFP mRNA (mmRNA) species, incorporating degrees 5-methylcytidine-5'-triphosphate (5mC) pseudouridine-5'-triphosphate (Ψ). We then investigated...
Angiogenesis plays a central role in the healing process following acute myocardial infarction. The PET tracer [68Ga]-NODAGA-RGD, which is ligand for αvβ3 integrin, has been investigated imaging angiogenesis of myocardium both animal and clinical studies. It´s value as prognostic marker functional outcome remains unclear. Therefore, aim this work was to establish [68Ga]-NODAGA-RGD murine infarct model evaluate predictor cardiac remodeling context stem cell therapy. performed seven days after...
The availability of truly maturated cardiomyocytic subtypes is a major prerequisite for cardiovascular cell replacement therapies. Pluripotent stem cells provide suitable source the development new strategies to overcome enormous hurdles such as yield, purity and safety in vitro generated cells.To address these issues, we have refined existing forward programming protocols by combining forced exogenous overexpression early transcription factor Nkx2.5 with αMHC-promoter-based antibiotic...
The data presented in this article are related to the research "Intramyocardial Fate and Effect of Iron Nanoparticles co-injected with MACS® purified Stem Cell Products" (Müller et al., 2017) [1]. This complements cellular localization superparamagnetic iron dextran particles (MACS® MicroBeads) used for magnetic activated cell sorting (MACS®). Data evaluate time-dependent detachment these nanoparticles from CD133+ haematopoietic stem cells (HSCs) CD271+ mesenchymal (MSCs). Furthermore,...
Aim . CD133 + stem cells bear huge potential for regenerative medicine. However, low retention in the injured tissue and massive cell death reduce beneficial effects. In order to address these issues, we intended develop a nonviral system appropriate engineering. Materials Methods Modification of human with magnetic polyplexes carrying microRNA was studied terms efficiency, safety, targeting potential. Results High uptake rates (~80–90%) were achieved without affecting properties. Modified...
To date, several stem cell types at different developmental stages are in the focus for treatment of degenerative diseases. Yet, certain aspects, such as initial massive death and low therapeutic effects, impaired their broad clinical translation. Genetic engineering cells prior to transplantation emerged a promising method optimize effects. However, safe efficient gene delivery systems still lacking. Therefore, development suitable methods may provide an approach resolve current challenges...
Umbilical cord blood (UCB) as a source of hematopoietic stem cells for transplantation is limited by the low number and delayed engraftment. UCB are infused i.v. transplantation, although only proportion reach BM. We investigated whether could be administered safely using superselective intra-arterial (i.a.) injection. injected human (5 × 106) into aorta in rats, iliac artery mice femoral nutrient (FNA) rabbits. used angiography, immunohistochemistry, intravital microscopy qPCR to assess...
While CD133+ hematopoietic stem cells (SCs) have been proven to provide high potential in the field of regenerative medicine, their low retention rates after injection into injured tissues as well observed massive cell death lead very restricted therapeutic effects. To overcome these limitations, we sought establish a non-viral based protocol for suitable engineering prior administration. The modification human expressing SCs using microRNA (miR) loaded magnetic polyplexes was addressed with...
O objetivo geral deste estudo consiste em propor um planejamento estrategico para uma empresa prestadora de servicos. A atividade abrange consertos, reparos e afericao balancas com capacidade estrutural ate 80.000kg, nos segmentos comercio, industria, farmacia, laboratorios na area da saude. Com base nas informacoes coletadas empresa, relacionado ao teorico sobre servicos, marketing planejameno estrategico, foi possivel indicar algumas sugestoes melhoria, o foco busca mercados atraves...
While CD133+ hematopoietic stem cells (SCs) have been proven to provide high potential in the field of regenerative medicine, their low retention rates after injection into injured tissues as well observed massive cell death lead very restricted therapeutic effects. To overcome these limitations, we sought establish a non-viral based protocol for suitable engineering prior administration. The modification human expressing SCs using microRNA (miR) loaded magnetic polyplexes was addressed with...