A5012794413- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Cardiac Fibrosis and Remodeling
- 3D Printing in Biomedical Research
- Connexins and lens biology
- Neuroscience and Neural Engineering
- Liver physiology and pathology
- Molecular Biology Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Virus-based gene therapy research
- Advanced Breast Cancer Therapies
- Integrated Circuits and Semiconductor Failure Analysis
- Genetic and Kidney Cyst Diseases
- Nicotinic Acetylcholine Receptors Study
- Pancreatic function and diabetes
- Advanced Electron Microscopy Techniques and Applications
- Signaling Pathways in Disease
- Jewish and Middle Eastern Studies
- Lung Cancer Research Studies
- Language, Linguistics, Cultural Analysis
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
University of Rostock
2015-2024
University Medical Center
2020-2023
Rostocker Zentrum zur Erforschung des Demografischen Wandels
2021
Canonical WNT/β-catenin signaling is a central pathway in embryonic development, but it also connected to number of cancers and developmental disorders. Here we apply combined in-vitro in-silico approach investigate the spatio-temporal regulation during early neural differentiation process human progenitors cells (hNPCs), which form new prospect for replacement therapies context neurodegenerative diseases. Experimental measurements indicate second signal mechanism, addition canonical WNT...
Tumors arising in the context of Lynch Syndrome or constitutional mismatch repair deficiency are hypermutated and have a good response towards immune-checkpoint inhibitors (ICIs), including α-PD-L1 antibodies. However, most cases, resistance mechanisms evolve. To improve outcomes prevent development, combination approaches warranted. Herein, we applied combined regimen with an antibody gemcitabine preclinical tumor model to activate endogenous antitumor immune responses. Mlh1−/− mice...
Mismatch repair-deficient (dMMR) tumors show a good response toward immune checkpoint inhibitors (ICI), but developing resistance impairs patients' outcomes. Here, we compared the therapeutic potential of an α-PD-L1 antibody with CDK4/6 inhibitor abemaciclib in two preclinical mouse models dMMR cancer, focusing on immune-modulatory effects either treatment. Abemaciclib monotherapy significantly prolonged overall survival Mlh1-/- and Msh2loxP/loxP;TgTg(Vil1-cre) mice (Mlh1-/-: 14.5 wks vs....
The initial idea of functional tissue replacement has shifted to the concept that injected cells positively modulate myocardial healing by a non-specific immune response transplanted within target tissue. This alleged local modification scar requires assessment regional properties left ventricular wall in addition commonly applied measures global morphological and parameters. Hence, we aimed at investigating effect cardiac cell therapy with cardiovascular progenitor cells, so-called induced...
Highly cytotoxic C7-modified colchicine analogues, exemplified by tubuloclustin, promote microtubule disassembly followed the formation of very stable tubulin clusters, both in vitro and cells. The proposed mechanism action tubuloclustin its beyond that colchicine, includes additional specific interactions with α-tubulin subunit.
Abstract Different subtypes of bone marrow-derived stem cells are characterized by varying functionality and activity after transplantation into the infarcted heart. Improvement cell therapeutics requires deep knowledge about mechanisms that mediate benefits treatment. Here, we demonstrated co-transplantation mesenchymal (MSCs) hematopoietic (HSCs) led to enhanced synergistic effects on cardiac remodeling. While HSCs were associated with blood vessel formation, MSCs found possess...
Ischemic heart failure still displays the highest mortality. An early boost of intracardiac regenerative key mechanisms and angiogenetic niche signaling in cardiac mesenchymal stem cells (MSCs) could improve myocardial infarction (MI) healing. Epicardial erythropoietin (EPO, 300U kg−1) was compared with intraperitoneal intramyocardial EPO treatments after acute MI rats (n=156). Real-time PCR confocal microscopy revealed epicardial treatment enhanced indicators (SDF-1, CXCR-4, CD34, Bcl-2,...
Cellular inflammation following acute myocardial infarction has gained increasing importance as a target mechanism for therapeutic approaches. We sought to investigate the effect of syngeneic cardiac induced cells (CiC) on using 18F-FDG PET (Positron emission tomography)-based imaging and resulting pump function magnetic resonance (CMR) in mouse model infarction. Mice underwent permanent left anterior descending coronary artery (LAD) ligation inducing an inflammatory response. The therapy...
The maturation of iPSC-derived cardiomyocytes is still a critical point for their application in cardiovascular research as well clinical use. Although multiple differentiation protocols have been established, researchers failed to generate fully mature vitro possessing identical phenotype-related and functional properties native adult counterparts. Besides electrophysiological metabolic changes, the establishment structured sarcomere network important development cardiac phenotype. Here, we...
The in vitro generation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC) is great importance for cardiac disease modeling, drug-testing applications and regenerative medicine. Despite the development various cultivation strategies, a sufficiently high degree maturation still decisive limiting factor successful application these cells. process includes, among others, proper formation sarcomere structures, mediating contraction cardiomyocytes. To precisely monitor...
CD133+ stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. Several clinical trials have shown remarkable beneficial effects following their intramyocardial transplantation. Yet, the purification of is typically performed centralized clean room facilities using semi-automatic manufacturing processes based on magnetic cell sorting (MACS®). However, this requires time-consuming and cost-intensive logistics.CD133+ were purified from...
Abstract It was suggested that gap junctional intercellular communication ( GJIC ) and connexin C x) proteins play a crucial role in cell proliferation differentiation. However, the mechanisms of coupling regulating fate during embryonic development are poorly understood. To study differentiation, we used human neural progenitor line derived from ventral mesencephalon. Fluorescence recovery after photobleaching FRAP showed dye extensive proliferating cells but diminished induction as...