A5043119514- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
- Air Quality and Health Impacts
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Atmospheric chemistry and aerosols
- Pancreatic function and diabetes
- Xenotransplantation and immune response
- Diabetes and associated disorders
- Receptor Mechanisms and Signaling
- Air Quality Monitoring and Forecasting
- Cardiovascular Health and Disease Prevention
- Cardiovascular Disease and Adiposity
- Tissue Engineering and Regenerative Medicine
- Cardiovascular Function and Risk Factors
- Occupational and environmental lung diseases
- Preterm Birth and Chorioamnionitis
- Atrial Fibrillation Management and Outcomes
- X-ray Spectroscopy and Fluorescence Analysis
- HIV-related health complications and treatments
- Renal Transplantation Outcomes and Treatments
- Congenital Anomalies and Fetal Surgery
- T-cell and B-cell Immunology
University of Florence
2024
Istituto Nazionale di Fisica Nucleare
2024
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2017-2023
Azienda USL di Bologna
2021-2022
University of Bologna
2021
Karolinska Institutet
2007-2018
Karolinska University Hospital
2003-2017
University of Milano-Bicocca
2010-2011
Organogenesis (United States)
2011
Fondazione Salvatore Maugeri
2008
Background and Objectives Mesenchymal stromal cells (MSC) may be used in cellular therapy to treat graft-versus-host-disease autoimmune disorders, regenerative medicine. Preliminary data suggest limited allogeneic rejection, but less is known about humoral responses. The objective of this study was investigate whether antibodies against MSC were present after hematopoietic stem cell transplantation (HSCT) including treatment with HLA matched or mismatched MSC.Design Methods Twelve patients...
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) have immunosuppressive properties and been used to treat steroid-refractory acute graft-versus-host disease (GVHD) in stem cell transplant patients. Cells with similar capacities can also be found term placental tissue. We isolated from fetal membrane (FMSCs), umbilical cords (UCSCs) villi (PVSCs) as well bone marrow compared their immunoregulatory capacity allogeneic settings. that FMSCs UCSCs suppressed proliferation significantly...
Abstract The placenta protects the fetus from mother's immune system. We have previously found that fetal membrane cells (FMCs) isolated term prevent alloreactivity in vitro. FMCs share many features with bone marrow-derived mesenchymal stromal (MSCs), which we introduced to treat severe acute graft-versus-host disease (GVHD). Here, tested for treatment of steroid-refractory GVHD. After two passages culture, approximately 109 were obtained one single placenta, although not all passage 0 and...
Suppression of immune reactivity by increased expression co-inhibitory receptors has been discussed as a major reason to why the system fails control tumor development. Elucidating pattern tumor-infiltrating lymphocytes in different cancer types will help develop future treatment strategies. We characterized markers reflecting and affecting T-cell functionality flow cytometry on isolated from blood, ascites advanced ovarian patients (n = 35). Significantly higher proportions CD4+ CD8+...
Background— Receptor tyrosine kinases that include vascular endothelial growth factor (VEGFR)-1, VEGFR-2, and Tie-2 regulate cardiovascular development physiological pathological angiogenesis. We were interested in the phenotypic functional characterization of peripheral blood cells expressing these receptors their therapeutic potential injury. Methods Results— VEGFR-1 + , VEGFR-2 constituted ≈3.0±0.2%, 0.8±0.5%, 2.0±0.3%, respectively, total population mononuclear blood. Phenotypic analysis...
Decidual stromal cells (DSCs) isolated from fetal membranes of term placentas are easily expanded and highly immunosuppressive in vitro. These express high levels integrins that importance homing to inflamed tissues. In this study, we investigated DSCs as a cellular therapy for chronic graft-versus-host disease (cGvHD), severe complication after allogeneic hematopoietic stem cell transplantation. Subsequent transplantation, three patients developed extensive cGvHD were treated with...
Event Abstract Back to Decidual Stromal Cells Promote Regulatory T and Suppress Alloreactivity in a Cell Contact-Dependent Manner Tom Erkers1*, Silvia Nava1, Jena Yosef1, Olle Ringdén1 Helen Kaipe1 1 Karolinska Institutet, Sweden We introduced the use of stromal cells from term fetal membrane (FMCs) patients with steroid refractory Graft-versus-host Disease. FMCs have many advantages compared bone marrow-derived mesenchymal (BM-MSCs), including an increased allosuppression, unlimited supply,...
During pregnancy, the maternal immune system must tolerate developing foetus, and yet retain a potent antimicrobial response to prevent infections. Mucosal associated invariant T (MAIT) cells recognize microbial-derived vitamin B metabolites presented on MR1 molecule, but their presence function at foetal-maternal interface is not known. We here isolated mononuclear from paired samples of peripheral blood (PB), intervillous (IVB), decidua parietalis (DP) following uncomplicated term...
HIV (Human Immunodeficiency Virus) infection continues to be a global public health concern, with millions of people living the virus around world, largely due advances in Antiretroviral Therapy (ART) associated ostensible prevention campaigns. However, if on one hand significant increase survival patients is observed use ART, other several studies warn adverse effects prolonged ART metabolic system, particularly induction persistent hyperglycemic and hypercholesterolemic state, disorders...
SUMMARY Patients with IgA deficiency often demonstrate circulating antibodies against IgA, which have been suggested to be associated transfusion reactions. Sera from three patients common variable immunodeficiency (CVID) and one a selective anti-IgA receiving subcutaneous gammaglobulin replacement therapy were analysed for serum levels of IgG, before during treatment period 4–7 years. Treatment preparations containing significant amounts (< 5 mg/ml) resulted in decrease or...
Abstract During the last years considerable attention has been focused on possibility of development an oral vaccine against Helicobacter pylori. However, infection is known to be life-long, despite a vigorous immune response, and hypothesis that increased local production secretory IgA in gastric mucosa, due vaccination, should protect colonization bacterium may therefore questioned. In this study, when comparing seropositivity titre H. pylori IgA-deficient patients age-related normal blood...
Background. Transplantation of adult porcine islets (APIs) offers a possible means treating diabetes. However, isolating APIs has been notoriously difficult. Furthermore, islet xenograft rejection must be prevented. Materials and Methods. were isolated by modified automated method. API quality was assessed static glucose stimulation (SGS), transplantation to diabetic nude mice intraperitoneal tolerance tests (IPGTTs). The morphologic characteristics in rats studied immunohistochemically....
Multipotent mesenchymal stromal cells (MSCs) have immunosuppressive capacity but the exact mechanism by which they suppress proliferation of T lymphocytes is not fully understood. Recently, characteristics and function regulatory (Tregs) become better defined. Tregs MSCs features in common. Here, we looked for a common basis immunosuppression these distinct cell types. Forkhead box P3 (FoxP3) CD39 expression was measured flow cytometry real-time quantitative polymerase chain reaction. The...
The placenta protects the fetus from mother's immune system. We have previously found that fetal membrane cells (FMCs) isolated term prevent alloreactivity in vitro. FMCs share many features with bone marrow-derived mesenchymal stromal (MSCs), which we introduced to treat severe acute graft-versus-host disease (GVHD). Here, tested for treatment of steroid-refractory GVHD. After two passages culture, approximately 109 were obtained one single placenta, although not all passage 0 and 1 used...
Abstract B cell activating factor (BAFF) is a critical cytokine for maturation of immature cells. In murine lymph nodes, BAFF mainly produced by podoplanin-expressing stromal We have previously shown that circulating levels are maximal at birth, and farmers’ children exhibit higher in cord blood than non-farmers’ children. Here, we sought to investigate whether maternal-derived decidual cells from placenta secrete examine what factors could stimulate this production. found podoplanin...
Liver sinusoidal endothelial cells (LSECs) may be implicated in the induction of liver allograft rejections. We studied clinical consequences LSEC-reactive antibodies and their functional capacity modulating T-cell responses during acute Pre- posttransplant sera T lymphocytes from 95 transplant patients were used this study. LSECs isolated normal healthy liver. Binding to was detected using flow cytometry. To study whether LSEC facilitated cell-mediated immunity, a mixed cell culture (MCC)...
Allogeneic mesenchymal stromal cells (MSCs) are widely used in regenerative medicine, but little is known about their immunogenicity. In this study, we monitored the therapeutic and immunogenic effects of decidual (DSCs) from term placentas when as a therapy for generalized severe junctional epidermolysis bullosa (JEB) (previously termed Herlitz JEB), lethal condition caused by lack functional laminin-332. An 11-month-old JEB patient was treated with five infusions allogeneic DSCs within...
Selective administration of mesenchymal stromal cells to the mesenteric arteries is a potential technique overcome pulmonary trapping and increase density transplanted in extensive mural inflammation intestine, such as inflammatory bowel disease graft-versus-host disease. We injected 5 × 10 6 111 In-oxine-labeled human decidual (DSCs) rabbit superior artery (SMA) using clinical routine catheters guided by an angiographical system under sterile conditions. used longitudinal single-photon...