A5018698317- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Hematopoietic Stem Cell Transplantation
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Virus-based gene therapy research
- Prostate Cancer Treatment and Research
- Cytomegalovirus and herpesvirus research
- Selenium in Biological Systems
- Pancreatic function and diabetes
- Redox biology and oxidative stress
- Mesenchymal stem cell research
- Reproductive System and Pregnancy
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Inflammasome and immune disorders
- Inflammatory Biomarkers in Disease Prognosis
- Genomics, phytochemicals, and oxidative stress
- Monoclonal and Polyclonal Antibodies Research
Karolinska Institutet
2014-2024
Karolinska University Hospital
2014-2015
Suppression of immune reactivity by increased expression co-inhibitory receptors has been discussed as a major reason to why the system fails control tumor development. Elucidating pattern tumor-infiltrating lymphocytes in different cancer types will help develop future treatment strategies. We characterized markers reflecting and affecting T-cell functionality flow cytometry on isolated from blood, ascites advanced ovarian patients (n = 35). Significantly higher proportions CD4+ CD8+...
γδ T cells are clonotypically distinct in ascites and tumors of patients with ovarian cancer their functionality correlates clinical outcome.
Benign prostatic hyperplasia (BPH) is a common chronic non-malignant condition whose prevalence substantially increases with age. Immune cell infiltration and pro-inflammatory mediators have been implicated in the pathogenesis. Here, we characterized 21 extracellular markers on prostate-infiltrating lymphocytes (PILs) analyzed expression of 26 soluble proteins prostate tissue obtained from BPH patients (n = 31). These data were correlated clinical parameters compared peripheral blood...
Allogeneic hematopoietic stem cell transplantation is associated with several complications and risk factors, for example, graft versus host disease (GVHD), viral infections, relapse, rejection. While high levels of CD3+ cells in grafts can contribute to GVHD, they also promote the leukemia (GVL) effect. Infusions extra lymphocytes from original donor be used as a treatment after relapse or poor immune reconstitution but increase GVHD. In peripheral blood, 95% T-cells express the<mml:math...
Abstract Background Intratumoral injection of oncolytic viruses (OVs) shows promise in immunotherapy: ONCOS-102, a genetically engineered OV that encodes Granulocyte–Macrophage Colony-Stimulating Factor (GM-CSF) demonstrated efficacy early clinical trials, enhancing T cell infiltration tumors. This suggests OVs may boost various forms immunotherapy, including tumor-specific bi-specific antibodies (BsAbs). Methods Our study investigated vitro, how ONCOS-204, variant ONCOS-virus expressing the...
Chronic graft-versus-host disease (cGVHD) is a debilitating complication arising in around half of all patients treated with an allogeneic hematopoietic stem cell transplantation. Even though treatment severe cGVHD has improved during recent years, it remains one the main causes morbidity and mortality affected patients. Biomarkers blood that could aid diagnosis classification severity are needed for development novel strategies can alleviate symptoms reduce need painful sometimes...
The role of gamma delta ( γδ ) T cells in human cytomegalovirus (HCMV) immune surveillance has been the focus research interest for years. Recent reports have shown a substantial clonal proliferation response to HCMV, shedding light on adaptive cells. Nevertheless, most efforts focused V δ 2 neg cell subset while less attention given investigate other common subsets. In this regard, distinct subpopulation that expresses CD8 coreceptor (CD8 + cells) not thoroughly explored. Whether it is...
Redox active selenium (Se) compounds at sub toxic doses act as pro-oxidants with cytotoxic effects on tumor cells and are promising future chemotherapeutic agents. However, little is known about how Se affect immune in the microenvironment. We demonstrate that inorganic compound selenite organic methylseleninic acid (MSA) do not, despite their pro-oxidant function, influence viability of cells, gives ovarian cancer cell lines. Treatment line A2780 MSA increases NK mediated lysis, enhances...
Allogeneic hematopoietic stem cell transplantation (HSCT) is associated with several potentially lethal complications. Higher levels of CD3+ T-cells in the graft have been increased risk graft-versus-host disease (GVHD), but also beneficial graft-versus-leukemia effect and reduced infections. To tackle post-transplant complications, donor lymphocyte infusions used an GVHD. reduce this risk, we performed depletion αβ treated 12 patients post-HSCT suffering from infections and/or poor immune...
Immune checkpoint blockade has shown promising results in numerous cancer types. However, prostate (PC), absent or limited responses have been reported. To investigate further, we compared the phenotype of infiltrating T-cells isolated from tissue patients with PC (n = 5), benign prostatic hyperplasia (BPH) 27), BPH concurrent 4) and controls 7). The majority were CD8+ had a CCR7-CD45RO+ effector memory phenotype. yield lesions was on average 20-fold higher than that obtained control...
BACKGROUND Benign prostatic hyperplasia (BPH) is the most common benign adenoma and prostate cancer frequent malignancy in men over 50 years of age Western world, where it remains a significant health problem. Prostate lesions are known to contain immune cells, which may contribute control tumor progression. However, due their low numbers restricted access necessary material difficult isolate cells from tissue characterize immunological features. METHODS An efficient robust method was...
PD-1/PD-L1 blockade has revolutionized the field of immunooncology. Despite relative success, response rate to anti-PD-1 therapy requires further improvements. Our aim was explore enhancement T-cell function by using novel PD-1-blocking proteins and compare with clinically approved monoclonal antibodies (mAbs). We isolated T-cells from ascites tumor 17 patients advanced epithelial ovarian cancer (EOC) analyzed effects mAbs nivolumab pembrolizumab two engineered ankyrin repeat (DARPin®...
Abstract Background. Intratumoral injection of oncolytic viruses (OVs) shows promise in immunotherapy: ONCOS-102, a genetically engineered OV that encodes Granulocyte-macrophage colony-stimulating factor (GM-CSF) demonstrated efficacy early clinical trials, enhancing T cell infiltration tumors. This suggests OVs may boost various forms immunotherapy, including tumor-specific bi-specific antibodies (BsAbs). Methods. Our study investigated vitro, how ONCOS-204, variant ONCOS-virus expressing...
Allogeneic hematopoietic stem cell transplantation (HSCT) is commonly used as a treatment for hematological malignancies, immunodeficiencies, and inborn errors of metabolism. HSCT associated with several complications risk factors, example graft-versus-host disease (GVHD), viral infections, relapse, graft rejection. Infusions extra lymphocytes from the original donor can be after for, e.g., poor immune reconstitution or secondary failure. The term booster applies infusions cells without...
Chronic graft-versus-host-disease (cGvHD) is a late complication (>90 days) after allogeneic hematopoietic stem cell transplantation (HSCT). Symptoms might occur in multiple organs and vary from mild to severe. In this study, the NIH criteria were used group patients. We performed an extensive immune-phenotypic analysis using cells plasma patients diagnosed with without cGvHD. The aim of study gain information on possible markers that could be by clinicians aid diagnosis
Chronic graft versus host disease (cGvHD) is a late complication (>90 days) post hematopoietic stem cell transplantation (HSCT). Multiple organs are affected and symptoms vary from mild to severe. We performed an extensive immune-phenotypic analysis on patients diagnosed with varying grades of cGvHD (according NIH criteria).
<h3>Background</h3> We have previously demonstrated successful delivery of a granulocyte-macrophage colony-stimulating factor (GM-CSF) payload to solid tumors through the adenoviral vector ONCOS-102, resulting in robust clinical benefit hallmarked by increased T-cell infiltration and activation immune-related genetic pathways (e.g., cytotoxicity co-stimulatory gene ontologies). Persistence ONCOS-102 durability GM-CSF expression tumor biopsies correlated with positive outcomes, suggesting...