A5024043043- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Allergic Rhinitis and Sensitization
- Hematopoietic Stem Cell Transplantation
- Asthma and respiratory diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Eosinophilic Disorders and Syndromes
- Chronic Lymphocytic Leukemia Research
- Dermatology and Skin Diseases
- Food Allergy and Anaphylaxis Research
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Response and Inflammation
- Contact Dermatitis and Allergies
- Kruppel-like factors research
- Acute Myeloid Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Mast cells and histamine
- Cytomegalovirus and herpesvirus research
- Bacterial Infections and Vaccines
- Synthesis and Biological Evaluation
- Corneal Surgery and Treatments
- Complement system in diseases
- Pharmacogenetics and Drug Metabolism
KTH Royal Institute of Technology
2017-2023
Science for Life Laboratory
2017-2023
Karolinska Institutet
2005-2020
Karolinska University Hospital
2007-2020
Metropolitan University
2008-2009
ENT and Allergy
2008
The mechanisms by which immune responses to nonpathogenic environmental antigens lead either allergy or nonharmful immunity are unknown. Single allergen-specific T cells constitute a very small fraction of the whole CD4+ cell repertoire and can be isolated from peripheral blood humans according their cytokine profile. Freshly purified interferon-gamma-, interleukin (IL)-4-, IL-10-producing display characteristics helper (Th)1-, Th2-, regulatory (Tr)1-like cells, respectively. Tr1...
Summary Background CD4 + CD25 regulatory T (Treg) cells and the cytokines IL‐10 or TGF‐β play key roles in maintenance of cell homeostasis tolerance to infectious non‐infectious antigens such as allergens. Objective To investigate regulation immune responses birch pollen allergen compared with influenza antigen by Treg obtained from pollen‐allergic patients non‐allergic controls. Methods Peripheral blood was collected 10 healthy − isolated magnetic‐activated sorting were co‐cultured...
Background Asthma is a respiratory tract disorder characterized by airway hyper-reactivity and chronic inflammation. Allergic asthma associated with the production of allergen-specific IgE expansion T-cell populations. Progression allergic inflammation driven T-helper type 2 (Th2) mediators alterations in levels lipid mediators. Objectives Responses system to birch allergen provocation asthmatics were investigated. Eicosanoids other oxylipins quantified bronchoalveolar lumen provide measure...
Recently, recombinant hypoallergenic derivatives of the major birch pollen allergen, Bet v 1, were used to treat birch-pollen-allergic patients in a double-blind, placebo-controlled, multi-centre immunotherapy study. The aim this study was evaluate effects vaccination with aluminium-hydroxide-adsorbed 1 versus placebo on T-cell, cytokine and antibody responses subgroup patients.Blood drawn from Swedish centre (n = 27; rBet fragments: n 10; trimer: 8, placebo-aluminium hydroxide: 9) before...
Allergic asthma is caused by allergen-specific IgE and T-helper cell (Th) type 2 responses towards airborne allergens. The objective of this study was to investigate local systemic regulatory mechanisms in the early asthmatic response bronchial allergen provocation.Birch pollen-allergic patients with mild (n = 13) healthy nonallergic controls 14) were subjected bronchoalveolar lavage (BAL) blood sampling. On BAL performed twice: without preceding provocation ('before samples') 24 h after...
Background: Allergen‐specific immunotherapy (ASIT) is the only treatment of allergic disease that gives long‐lasting relief symptoms. However, concerns for safety and efficiency have highlighted need improvement therapy. We previously suggested carbohydrate‐based particles (CBPs) as a novel adjuvant allergen carrier ASIT. Our aim this study was to evaluate therapeutic potential CBPs in ASIT, employing mouse model cat allergy. Methods: BALB/c mice were subcutaneously immunized with...
Allogeneic hematopoietic stem cell transplantation is associated with several complications and risk factors, for example, graft versus host disease (GVHD), viral infections, relapse, rejection. While high levels of CD3+ cells in grafts can contribute to GVHD, they also promote the leukemia (GVL) effect. Infusions extra lymphocytes from original donor be used as a treatment after relapse or poor immune reconstitution but increase GVHD. In peripheral blood, 95% T-cells express the<mml:math...
Defined particles carrying tightly bound allergens at high density have been suggested as alternatives in allergy vaccination. Carbohydrate based (CBP), sized 2 microm, provide a platform for covalent coupling of allergens.To investigate the mechanisms antigen presentation by CBP, well cellular and humoral responses after vaccination with major cat allergen Fel d 1, covalently coupled to CBP.Mice (n = 10/group) were subcutaneously vaccinated CBP-rFel CBP or phosphate buffer saline (PBS)...
Cord blood (CB) as a source of stem cells has been successful addition to the field allogeneic cell transplantation (ASCT). The increased human leukocyte antigen (HLA) permissiveness CB grafts made it possible for more patients undergo treatment. drawback is that suffer from longer period compromised immunity. We analyzed T-cell receptor excision circles (TRECs), immunoglobulin G (IgG), and M (IgM) levels after cord (CBT) in 50 transplanted at our center. These immunological parameters were...
Leukemic stem cells (LSCs) may harbor important resistance to tyrosine kinase inhibitors in chronic myelogenous leukemia (CML). We identified Philadelphia chromosome (Ph)-positive CD34(+)CD38(-) bone marrow (here denoted LSCs) and addressed their response-predictive value patients with CML (n = 48) subjected nilotinib the ENEST1st trial (NCT01061177).Two flow cytometry-based cell sorting methods were used multiparameter-directed CD45- (MPFC) BCR-ABL1 probe-linked (FISH) identification of...
Summary A group of European FOCIS Centers Excellence adapted panels the Human Immunophenotyping Consortium (HIPC) for whole blood analysis. Using four core [T/regulatory T cell/B/natural killer (T/Treg/B/NK) and myeloid cells] main leukocyte populations were analyzed in a clinical–diagnostic setting harmonized manner across different platforms. As first step, consortium presents here absolute relative frequencies subpopulations peripheral more than 300 healthy volunteers six centers.
Summary Inflammatory bowel disease (IBD) can be treated effectively by anti-tumour necrosis factor (TNF) therapy. We set out to investigate the unclear immunoregulatory mechanisms of treatment. Thirty-four patients with IBD anti-TNF were included. Lymphocytes from peripheral blood and intestinal biopsies analysed flow cytometry. Regulation antigen-stimulated proliferation was blocking interleukin (IL)-10, transforming growth (TGF)-β or depletion CD25+ cells in mononuclear cell cultures. No...
γδ T cells play a pivotal role in protection against various types of infections and tumours, from early childhood on throughout life. They consist several subsets characterised by adaptive innate-like functions, with Vγ9Vδ2 being the largest subset human peripheral blood. Although these show signs cytotoxicity, their modus operandi remains poorly understood. Here we explore, using live single-cell imaging, cytotoxic functions upon interactions tumour target high temporal spatial resolution....
Allogeneic hematopoietic stem cell transplantation (HSCT) is associated with several potentially lethal complications. Higher levels of CD3+ T-cells in the graft have been increased risk graft-versus-host disease (GVHD), but also beneficial graft-versus-leukemia effect and reduced infections. To tackle post-transplant complications, donor lymphocyte infusions used an GVHD. reduce this risk, we performed depletion αβ treated 12 patients post-HSCT suffering from infections and/or poor immune...
Tick-borne encephalitis (TBE) is a viral infection of the CNS caused by TBE virus. With no specific treatment available, only protection formalin-inactivated whole virus vaccine. Primary immunization with European vaccines, as recommended manufacturers, consists three vaccine doses administered within 1-y period. Protection from vaccination believed to be mediated Abs, yet T cells may also have protective role. We set out characterize human CD4+ cell response throughout primary immunization....
<i>Background:</i> Endotoxins, comprised of bacterial cell wall lipopolysaccharides (LPS), have been reported to both protective and exacerbating effects on the development maintenance allergic disease in humans markers inflammation animal models allergy. In this study, we investigated effect low concentrations LPS human peripheral blood mononuclear cells (PBMC) stimulated with major cat allergen Fel d 1. <i>Methods:</i> Extensive purification recombinant (r) 1...
Background and Objectives Allogeneic hematopoietic stem cell transplantation ( HSCT ) is a routine clinical procedure performed to treat patients with haematological malignancies, primary immune deficiencies or metabolic disorders. Infections during lymphopenia after allogeneic are associated high mortality morbidity. Typical infectious agents Epstein–Barr virus, cytomegalovirus, herpes simplex varicella‐zoster virus fungi. The study aim was evaluate whether measurement of the responses...
Adoptive transfer of alloantigen-specific immunomodulatory cells generated ex vivo with anti-CD80/CD86 mAbs (2D10.4/IT2.2) holds promise for operational tolerance after transplantation. However, good manufacturing practice is required to allow widespread clinical application. Belatacept, a clinically approved cytotoxic T-lymphocyte antigen 4-immunoglobulin that also binds CD80/CD86, could be an alternative agent 2D10.4/IT2.2. With the goal generating optimal cell treatment reagents, we...
Allogeneic hematopoietic stem cell transplantation (HSCT) is commonly used as a treatment for hematological malignancies, immunodeficiencies, and inborn errors of metabolism. HSCT associated with several complications risk factors, example graft-versus-host disease (GVHD), viral infections, relapse, graft rejection. Infusions extra lymphocytes from the original donor can be after for, e.g., poor immune reconstitution or secondary failure. The term booster applies infusions cells without...