A5088539282- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- Rheumatoid Arthritis Research and Therapies
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Inflammatory Myopathies and Dermatomyositis
- HER2/EGFR in Cancer Research
- Cytokine Signaling Pathways and Interactions
- Toxin Mechanisms and Immunotoxins
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Vasculitis and related conditions
- Viral Infections and Immunology Research
- Diabetes and associated disorders
- Lymphoma Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Systemic Sclerosis and Related Diseases
- Renal Diseases and Glomerulopathies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- vaccines and immunoinformatics approaches
- Atherosclerosis and Cardiovascular Diseases
Karolinska University Hospital
2016-2025
Karolinska Institutet
2016-2025
Swedish Rheumatism Association
2023
Leiden University Medical Center
2023
Svenska Örtmedicinska Institute
2017
Uppsala University
1989-2012
Metropolitan University
2006
Center for Rheumatology
2005
Lund University
1994-2002
University of Oxford
2000-2001
It is now clear that functionally specialized regulatory T (Treg) cells exist as part of the normal immune repertoire, preventing development pathogenic responses to both self- and intestinal antigens. Here, we report Treg control inflammation express same phenotype (CD25+CD45RBlowCD4+) those autoimmunity. Previous studies have failed identify how CD25+ function in vivo. Our reveal immune-suppressive these vivo dependent on signaling via negative regulator cell activation cytotoxic...
The integrin CD103 is highly expressed at mucosal sites, but its role in immune regulation remains poorly understood. We have analyzed the functional of intestinal using T cell transfer model colitis. Our results show no mandatory for expression on cells either development or CD4+CD25+ regulatory (T reg) cell–mediated control However, wild-type were unable to prevent colitis immune-deficient recipients lacking CD103, demonstrating a nonredundant host reg regulation. Non–T restricted...
Abstract In the homeostasis of immune system regulatory cells play a major role. Removal one group cells, CD25 + CD4 T leads to autoimmune manifestations in experimental animal models, and reintroduction this population prevents disease. This study addresses role such humans with an disease, where we demonstrate presence bright target organ patients active rheumatoid arthritis. The displayed enrichment synovial fluid as compared peripheral blood. These are functional they were able suppress...
CD25+CD4+ regulatory T cells participate in the regulation of immune responses. We recently demonstrated presence CD25brightCD4+ with a capacity to control cell proliferation joints patients rheumatoid arthritis. Here, we investigate possible accumulation these inflamed joint different rheumatic diseases including The studies are also extended analyze whether cytokine production can be suppressed by cells. Synovial fluid and peripheral blood samples were obtained during relapse from 36...
Rheumatoid arthritis (RA)-specific anti-citrullinated protein/peptide antibodies (ACPAs) appear before disease onset and are associated with bone destruction. We aimed to dissect the role of ACPAs in osteoclast (OC) activation identify key cellular mediators this process.Polyclonal ACPA were isolated from synovial fluid (SF) peripheral blood patients RA. Monoclonal single SF B-cells OCs developed cell precursors or without ACPAs. analysed expression citrullinated targets peptidylarginine...
Abstract Immunglobulin G (IgG) sialylation represents a key checkpoint that determines the engagement of pro- or anti-inflammatory Fcγ receptors (FcγR) and direction immune response. Whether IgG influences osteoclast differentiation subsequently bone architecture has not been determined yet, but may represent an important link between activation loss. Here we demonstrate desialylated, sialylated, complexes enhance osteoclastogenesis in vitro vivo . Furthermore, find Fc state random specific...
Objective An interesting and so far unexplained feature of chronic pain in autoimmune disease is the frequent disconnect between inflammation. This illustrated well rheumatoid arthritis (RA) where joints (arthralgia) may precede joint inflammation persist even after successful anti-inflammatory treatment. In present study, we have addressed possibility that autoantibodies against citrullinated proteins (ACPA), RA, be directly responsible for induction pain, independent Methods Antibodies...
Monocytes are highly abundant circulatory effector cells and play a vital role in driving or resolving inflammatory processes depending on their activation phenotype. We investigated compared panel of polarization protocols blood-derived monocytes to achieve stable, optimal effective regimen for vitro induction immunosuppressive human macrophages, evaluating surface receptor expression, cytokine profile, scavenging function ability suppress T-cell proliferation. Importantly, we assessed the...
Rheumatoid arthritis (RA) is thought to be a T cell-mediated disease, based on its strong association with HLA class II alleles, clinical responsiveness cell-directed therapies, and the presence of CD4+ cells in rheumatoid joints. The anti-citrullinated protein antibodies (ACPAs) RA serum these HLA-DR4 alleles implicate citrulline-specific autoreactive development progression RA. goal this study was determine characteristics specificity cell responses RA.We developed panel HLA-DRB1*04:01...
Abstract Rheumatoid arthritis (RA) is an autoimmune disease affecting synovial joints where different CD4 + T cell subsets may contribute to pathology. Here, we perform single sequencing on cells from anti-citrullinated protein antibodies (ACPA)+ and ACPA- RA patients identify two peripheral helper (T PH ) states a cytotoxic subset. We show that the adhesion G-protein coupled receptor 56 (GPR56) delineates CXCL13 high expressing LAG-3 tissue-resident memory receptors CXCR6 CD69. In SF,...
Abstract Transfer of CD45RBhigh CD4+ T cells to immune-deficient mice in the absence regulatory leads a Th1-mediated colitis. In this study, we show that intestinal inflammation is characterized by 15-fold increase number CD134L+ (OX40L+)-activated DC mesenteric lymph nodes (MLNs) compared with BALB/c mice. This was important functionally, as administration an anti-CD134L mAb inhibited proliferation MLNs well their expression gut-homing integrin α4β7. Most importantly, completely blocked...
A critical event in an immune response is the T cell recognition of peptides bound to major histocompatibility complex (MHC) molecules on surface antigen presenting (APC). Although majority eukaryotic proteins are glycosylated, it has not yet been shown that such involves carbohydrates. Type II collagen (CII), protein constituent joint cartilage, posttranslationally modified by hydroxylation and glycosylation lysines. In this report we show posttranslational modifications immunodominant...
Abstract Objective High titers of specific anti–citrullinated protein antibodies (ACPAs) are frequently present in the serum rheumatoid arthritis (RA) patients, but their presence synovial fluid is less well characterized. The purpose this study was to compare levels antibody 4 well‐defined citrullinated candidate RA autoantigens and determine whether one antigen dominant over another. Furthermore, we studied relationships with mutated vimentin (MCV), a newly identified RA‐specific assay,...
Antibodies targeting citrullinated proteins (ACPAs [anticitrullinated protein antibodies]) are commonly found in patients with rheumatoid arthritis (RA), strongly associate distinct HLA-DR alleles, and predict a more aggressive disease course as compared seronegative patients. Still, many features of these antibodies, including their site production the extent MHC class II-driven T cell help, remain unclarified. To address questions, we have used single B cell-based cloning technology to...
Autoantibodies to cyclic citrullinated peptides (anti-CCP) are present in most patients with rheumatoid arthritis (RA), and associate HLA-DRB1 shared epitope (SE) alleles.To investigate reactivities of anti-CCP various proteins/peptides, which represent potential autoantigens RA, examine the relationship between such antibodies, their association genetic variants within SE alleles.Serum samples from 291 established RA 100 sex- age-matched healthy subjects were included this study. Sera first...
Abstract The objective of this study was to investigate the interaction between levels BAFF (B-cell activation factor tumour necrosis [TNF] family) and APRIL (a proliferation-inducing ligand) B-cell frequencies in patients with systemic lupus erythematosus (SLE) rheumatoid arthritis (RA) treated B-cell-depleting agent rituximab. Ten SLE were rituximab combination cyclophosphamide corticosteroids. They followed longitudinally up 6 months after repopulation. Nine RA, resistant or intolerant...
<h3>Objective</h3> To investigate the role of genetic and environmental factors in development anticitrullinated protein antibodies (ACPA) ACPA-positive rheumatoid arthritis (RA) a twin cohort. <h3>Methods</h3> A total 12 590 twins were analysed for presence ACPAs (CCP2 ELISA), HLA-DRB1 shared epitope (SE) gene alleles, exposure to smoking. Twins with established RA identified national public care registers. Antibody reactivities against citrullinated native forms α-enolase, vimentin,...