A5083935487- Malaria Research and Control
- Complement system in diseases
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Parasites and Host Interactions
- Invertebrate Immune Response Mechanisms
- HIV Research and Treatment
- Allergic Rhinitis and Sensitization
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- Drug Transport and Resistance Mechanisms
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Asthma and respiratory diseases
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Bladder and Urothelial Cancer Treatments
- Immune responses and vaccinations
- Contact Dermatitis and Allergies
- Child Nutrition and Water Access
- Vector-borne infectious diseases
Stockholm University
2013-2024
Wenner-Gren Foundations
2008-2020
Noguchi Memorial Institute for Medical Research
2002-2008
University of Ghana
2002-2008
Korle Bu Teaching Hospital
2008
University of Bamako
2005
Centre National de Recherche et de Formation sur le Paludisme
2005
Sapienza University of Rome
2005
Kenya Medical Research Institute
2004
Moi University
2004
Infectious diseases exert a constant evolutionary pressure on the genetic makeup of our innate immune system. Polymorphisms in Toll-like receptor 4 (TLR4) have been related to susceptibility Gram-negative infections and septic shock. Here we show that two polymorphisms TLR4, Asp299Gly Thr399Ile, unique distributions populations from Africa, Asia, Europe. Genetic functional studies are compatible with model which nonsynonymous polymorphism has evolved as protective allele against malaria,...
Contribution of Vγ9/Vδ2 T lymphocytes to immune protection against Mycobacterium tuberculosis is still a matter debate. It was reported earlier that kill macrophages harboring live M. through granule-dependent mechanism results in killing intracellular bacilli. This study found reduce the viability both extracellular and tuberculosis. Granulysin perforin, detected lymphocytes, play major role, which indicates directly contribute protective host response infection
Individuals living in malaria endemic areas generally harbour multiple parasite strains. Multiplicity of infection (MOI) can be an indicator immune status. However, whether this is good or bad for the development immunity to malaria, still a matter debate. This study aimed examine MOI asymptomatic children between two and ten years age relate it erythrocyte variants, clinical attacks, transmission levels other parasitological indexes. Study took place Niakhar area Senegal, where mesoendemic...
Both iron deficiency and malaria are common in much of sub-Saharan Africa, the interaction between these conditions is complex. To investigate association nutritional status, immunoglobulins, clinical Plasmodium falciparum malaria, we determined incidence a cohort children ages 8 months years who were living on Kenyan coast. Biochemical status malaria-specific immune responses during 2 cross-sectional surveys. We found that was significantly lower among iron-deficient (incidence-rate ratio...
An effective immune response against the intracellular pathogen Mycobacterium tuberculosis is strictly dependent on T cell activation. Although this protective mainly depends local release of pro-inflammatory cytokines by Th1 CD4+ cells, contribution Vγ9 / Vδ2 lymphocytes to protection suggested antimycobacterial reactivity subset and its ability produce large amounts cytokines. Here we show that kill macrophages harboring live M. tuberculosis. The cytotoxic activity was not MHC class I or...
SUMMARY In the course of studying immunoregulation in human Plasmodium falciparum malaria we have investigated IgE levels and anti-plasmodial antibodies children adults from areas high endemicity both Africa Asia. On average, 85% all donors had significantly elevated total IgE. A fraction activity as revealed by ELISA with lysates infected erythrocytes antigen. Using synthetic peptides representing antigenic regions two major plasmodial blood stage antigens, antibody concentrations ranged 5...
Abstract Activation of Tcells results in intracellular expression and secretion cytokines such as interferon (IFN)‐γ. Here we have used three different assays for determination IFN‐γ tetanus toxoid‐ or mitogen‐activated human T cell cultures. Two these [intracytoplasmic immunofluorescence enzyme‐linked immuno spot assay (ELISPOT)] determined the at single‐cell level while third immunosorbent (ELISA) measured secreted into culture supernatant. Comparison all tests revealed a good correlation...
Summary Nickel (Ni), the main cause of contact allergy to metals, induces in vitro production both Th1- and Th2-type cytokines peripheral blood mononuclear cells (PBMC) from allergic subjects. Because knowledge cellular immune response other metals involved has been limited, we investigated cytokine profile induced by Ni, cobalt (Co), chromium (Cr), palladium (Pd) gold (Au) PBMC patients with patch test reactivity respective metals. Co, Cr, Au and/or Pd (n = 31) non-allergic controls 5) were...
ABSTRACT Infection with blood-stage Plasmodium chabaudi AS results in splenomegaly, peripheral leukocytosis, and a major activation of the immune system. The frequencies absolute numbers T-cell, B-cell, macrophage populations spleen blood from P. -infected BALB/c mice were compared found to be significantly altered during acute infection. kinetics redistribution different cell types different, T B cells appearing when their low. frequency number apoptotic increased infection involved both...
Mixed parasitic infections are common in many parts of the world. However, little is known about how concurrent affect immunity to and/or pathogenesis each other. Protection and elimination blood-stage Plasmodium chabaudi AS resistant mice characterized by a sequential activation CD4(+) Th1 Th2 cells. The patent egg-laying stage murine model Schistosoma mansoni associated with strong response both unrelated antigens. In this study, we investigated infection S. would immune P. infection....
T cells play a crucial role in antibody-mediated and antibody-independent immunity against Plasmodium falciparum malaria. Therefore, vaccine immunogen should include parasite-derived B- T-cell epitopes capable of giving rise to protective responses both systems. The P. antigen Pf155/ring-infected erythrocyte surface (RESA), candidate, contains immunodominant T- B-cell located the central (5') C-terminal (3') invariant repeat regions molecule. To relate Pf155/RESA-peptide-specific function,...
Most children and adults living in areas where the endemicity of Plasmodium falciparum malaria is high have significantly elevated levels both total immunoglobulin E (IgE) IgE antimalarial antibodies blood. This elevation highest patients with cerebral malaria, suggesting a pathogenic role for this isotype. In study, we show that may also be seen severe without involvement parallels an tumor necrosis factor alpha (TNF). IgE-containing serum from immune donors was added to tissue culture...
Abstract The enzyme‐linked immunospot (ELISPOT) assay has been proven to be an efficient and sensitive method for the enumeration of single cells secreting antibodies or cytokines. Here we have used this determine number interleukin‐4 (IL‐4)‐ interferon‐γ (IFN‐γ)‐producing in vitro secondary responses tetanus toxoid (TT) mycobacterial antigen (purified protein derivative; PPD) mitogen phytohemagglutinin (PHA). PHA‐induced IL‐4 IFN‐γ secretion was well correlated suggesting polyclonal...
Adequate responses by our innate immune system toward invading pathogens were of vital importance for surviving infections, especially before the antibiotic era. Recently, a polymorphism in Mal (Ser180Leu, TIRAP rs8177374), an important adaptor protein downstream Toll-like receptor (TLR) 2 and 4 pathways, has been described to provide protection against broad range infectious pathogens. We assessed functional effects this human experimental endotoxemia, we demonstrate that individuals...
Immunity induced by Plasmodium vivax infections leads to memory T-cell recruitment and activation during subsequent infections. Here, we investigated the role of regulatory T cells (Treg) in coordination with host immune response P. infection. Our results showed a significant increase percentage FOXP3+ Treg, IL-10-secreting Type I Treg (Tr1) IL-10 levels patients acute infection as compared those found either naïve or controls. The concurrent population could also be reproduced vitro using...