A5039175745- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Vector-borne infectious diseases
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Renal Transplantation Outcomes and Treatments
- Salivary Gland Disorders and Functions
- Immunodeficiency and Autoimmune Disorders
- Viral Infections and Vectors
- Kawasaki Disease and Coronary Complications
- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Systemic Lupus Erythematosus Research
- Inflammasome and immune disorders
- Complement system in diseases
- Psoriasis: Treatment and Pathogenesis
- Chemokine receptors and signaling
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Systemic Sclerosis and Related Diseases
- Diabetes and associated disorders
University of Palermo
2015-2024
Azienda Ospedaliera Universitaria Policlinico "Paolo Giaccone" di Palermo
2018
University of Palermo
1998-2017
Tecnologie Avanzate (Italy)
2004-2016
Lambda Instruments (United States)
2007
Lionex (Germany)
2007
Guy's Hospital
2007
Istituto Zooprofilattico Sperimentale Della Sicilia
2006
Veterinary Medicines Directorate
2006
The Ohio State University
2003
The aim of the study was to better characterise immunological origin and behaviour interleukin (IL)-23-responsive innate lymphoid cells (ILCs) in gut, synovial fluid (SF) bone marrow (BM) patients with ankylosing spondylitis (AS).ILC1, ILC2 ILC3 were determined characterised by confocal microscopy flow cytometry ileal BM biopsies, peripheral blood (PB) SF mononuclear obtained from AS controls. Mucosal vascular addressin cell adhesion molecule 1 (MADCAM-1), IL-7, IL-15 aggregates tissue...
Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and mount both immediate effector functions memory responses after microbial infection. However, how cells mediate different facets of a response remains unknown. Here, we show that the expression CD45RA CD27 defines four subsets human with distinctive compartmentalization routes. Naive CD45RA+CD27+ CD45RA−CD27+ express lymph node homing receptors, abound in nodes, lack functions. Conversely, CD45RA−CD27−...
Contribution of Vγ9/Vδ2 T lymphocytes to immune protection against Mycobacterium tuberculosis is still a matter debate. It was reported earlier that kill macrophages harboring live M. through granule-dependent mechanism results in killing intracellular bacilli. This study found reduce the viability both extracellular and tuberculosis. Granulysin perforin, detected lymphocytes, play major role, which indicates directly contribute protective host response infection
Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role and Th9 cells RA, however, not yet adequately studied. aim this study was evaluate expression IL-9-expressing RA patients.
An effective immune response against the intracellular pathogen Mycobacterium tuberculosis is strictly dependent on T cell activation. Although this protective mainly depends local release of pro-inflammatory cytokines by Th1 CD4+ cells, contribution Vγ9 / Vδ2 lymphocytes to protection suggested antimycobacterial reactivity subset and its ability produce large amounts cytokines. Here we show that kill macrophages harboring live M. tuberculosis. The cytotoxic activity was not MHC class I or...
D6 is a decoy and scavenger receptor for inflammatory CC chemokines. D6-deficient mice were rapidly killed by intranasal administration of low doses Mycobacterium tuberculosis. The death D6(-/-) was associated with dramatic local systemic response levels M. tuberculosis colony-forming units similar to control D6-proficient mice. showed an increased numbers mononuclear cells (macrophages, dendritic cells, CD4 CD8 T lymphocytes) infiltrating inflamed tissues lymph nodes, as well abnormal...
Objectives. To investigate the expression of IL-34 in labial salivary glands (LSGs) patients with primary SS (p-SS) and its role inducing a pro-inflammatory monocyte phenotype. Methods. LSG biopsies were obtained from 20 p-SS 10 non-Sjögren's sicca syndrome (n-SS). The IL-34, IL-1β, TNF-α, IL-17 IL-23 was assessed by real-time PCR. also investigated LSGs immunohistochemistry. frequencies subpopulations CD14+ monocytes evaluated flow cytometry among isolated mononuclear cells peripheral blood...
We used the Luminex Bead Array Multiplex Immunoassay to measure cytokines, chemokines and growth factors responses same antigens for RD1-based Interferon γ Release Assay (IGRA) test. Seventy-nine individuals, 27 active TB, 32 latent infection subsets, 20 individuals derivative purified protein (PPD) negative (subjects that do not have any indurative cutaneous reaction after 72 hrs of intradermal injection PPD) with other pulmonary disease were retrospectively studied. Forty-eight analytes...
Abstract The lungs are considered to have an impaired capacity contain infection by pathogenic mycobacteria, even in the presence of effective systemic immunity. In attempt understand underlying cellular mechanisms, we characterized γδ T cell population following intranasal with Mycobacterium bovis bacillus Calmette-Guérin (BCG). peak expansion at 7 days postinfection preceded 30 day αβ and bacterial count. expanded cells BCG-infected mice represents resident Vγ2 subset as well influx Vγ1...
Cytokines such as tumour necrosis factor (TNF)-α, interleukin (IL)-12, interferon (IFN)-γ, IL-23 and, more recently, IL-9, have been implicated in the initiation/maintenance of inflammation psoriasis and psoriatic arthritis (PsA). In present study we aimed to characterize role γδ T cells peripheral blood synovial fluid PsA patients investigate their response in-vitro stimulation with antigen or cytokines (IL-9 IL-23). isolated from mononuclear were analysed by flow cytometry evaluate...
Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of cells with phosphoantigen IL-21 leads to selective expression the transcription repressor Bcl-6 polarization toward a lymphocyte subset displaying features follicular B-helper (TFH) cells. TFH-like have predominant central memory (CD27+CD45RA−) phenotype express ICOS, CD40L CXCR5. Upon antigen activation, they secrete IL-4, IL-10 CXCL13,...
Gammadelta T cells and dendritic (DCs) participate in early phases of immune response against Mycobacterium tuberculosis. We investigated whether a close functional relationship exists between these two cell populations using an vitro coculture human system. Vgamma9Vdelta2 induce full maturation M. tuberculosis-infected immature DCs, as demonstrated by upregulation the costimulatory CD80, CD86, CD40, HLA-DR molecules on infected DCs after 24 h coculture. Reciprocally, induced substantial...
Long-term evolution of subclinical gut inflammation to overt Crohn's disease (CD) has been described in AS patients. The aim this study was evaluate macrophage polarization occurring the inflamed patients with AS.Twenty-seven HLA-B27(+) patients, 20 CD and 17 normal controls were consecutively enrolled. Classic M1 (iNOS(+)IL-10(-)), resolution phase (iNOS(+)IL-10(+)), M2 CD14(+) macrophages characterized by immunohistochemistry flow cytometry. Quantitative gene expression analysis IFN-γ,...
Summary The aim of this study was to elucidate more clearly the role interleukin (IL)-18 in modulating IL-22 pathway primary Sjögren's syndrome (pSS) patients and pSS-associated lymphomas. Minor salivary glands (MSGs) from with pSS non-specific chronic sialoadenitis (nSCS), parotid biopsies non-Hodgkin lymphomas (NHL) developed patients, were evaluated for IL-18, IL-22, receptor 1 (IL-22R1), binding protein (IL-22BP) signal transducer activator transcription-3 (STAT-3) expression. MSGs...
T helper 9 (Th9) cells and interleukin (IL)-9 are involved in the pathogenesis of several autoimmune diseases. The exact role IL-9 Th9 patients with systemic sclerosis (SSc) have not yet been studied adequately. IL-9, IL-9R, transcription factor PU.1 (PU.1), IL-4, thymic stromal lymphopoietin (TSLP) transforming growth (TGF)-β expression were assessed skin kidney biopsies SSc healthy controls (HC) by immunohistochemistry (IHC). cellular source was also analysed confocal microscopy analysis....