A5071860569- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Mosquito-borne diseases and control
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Vectors
- Neurobiology and Insect Physiology Research
- CAR-T cell therapy research
- Reproductive System and Pregnancy
- Light effects on plants
- Virus-based gene therapy research
- Vector-borne infectious diseases
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Hemophilia Treatment and Research
- Genetic Neurodegenerative Diseases
- Insect and Arachnid Ecology and Behavior
- Virology and Viral Diseases
- Monoclonal and Polyclonal Antibodies Research
- Parvovirus B19 Infection Studies
UniQure (Netherlands)
2019-2021
Karolinska Institutet
2015-2020
Karolinska University Hospital
2015-2018
Leiden University Medical Center
2010-2012
Leiden University
2011
Wageningen University & Research
2008
HLA-E is a nonclassical HLA class I molecule, which differs from classical molecules by its nonpolymorphic, conserved nature. Expression and function of in normal tissues solid tumors not fully understood. We investigated protein expression on tissue sections 420 ovarian cervical cancers found equal or higher levels than counterpart epithelia 80% the tumors. was strongly associated with components antigen presentation pathway, e.g., transporter processing (TAP), endoplasmic reticulum...
Currently, individuals with pre-existing neutralizing antibodies (NABs) against adeno-associated virus (AAV) above titer of 5 are excluded from systemic AAV-based clinical trials. In this study we explored the impact anti-AAV5 NABs on efficacy AAV5-based gene therapy. AMT-060 (AAV5-human FIX) was evaluated in 10 adults hemophilia B who tested negative for using a GFP-based assay. study, more sensitive luciferase-based assay, show that 3 those patients positive NABs. However, no relationship...
Tick-borne encephalitis virus (TBEV) is transferred to humans by ticks. The causes tick-borne (TBE) with symptoms such as meningitis and meningoencephalitis. About one third of the patients suffer from long-lasting sequelae after clearance infection. Studies immune response during TBEV-infection are essential understanding host responses for development therapeutics. Here, we studied in detail primary CD8 T cell TBEV acute TBE. Peripheral blood cells mounted a considerable assessed Ki67 CD38...
Huntington disease (HD) is a fatal, neurodegenerative genetic disorder with aggregation of mutant Huntingtin protein (mutHTT) in the brain as key pathological mechanism. There are currently no modifying therapies for HD; however, HTT-lowering hold promise. Recombinant adeno-associated virus serotype 5 expressing microRNA that targets HTT mRNA (AAV5-miHTT) development treatment HD promising results rodent and minipig models. To support clinical trial, toxicity studies were performed non-human...
Target cell recognition by CTLs depends on the presentation of peptides HLA class I molecules. Tumors and herpes viruses have adopted strategies to greatly hamper this peptide at important bottleneck, transporter TAP. Previously, we described existence a CD8(+) CTL subpopulation that selectively recognizes such TAP-deficient cells in mouse models. In study, show human counterpart subset is readily detectable healthy subjects. Autologous PBMC cultures were initiated with dendritic rendered...
Abstract Tick-borne encephalitis virus (TBEV) is a leading cause of viral meningoencephalitis in many parts Europe and eastwards Asia, with high morbidity often long-term neurologic sequelae. With no treatment available, studies the immune response to TBEV are essential for understanding immunopathogenesis tick-borne development therapeutics. We have previously demonstrated that CD8+ T cell responses peripheral blood patients acute peak at around 7 d after hospitalization neuroinvasive phase...
Pre-existing neutralizing antibodies (NAb) to adeno-associated virus (AAV) may diminish the efficacy of AAV-based therapies depending on titer. To support gene therapy studies in pigs, seroprevalence NAb AAV1, 2, 5, 6, 8, and 9 serotypes were assessed sera 3 different strains pigs consisting 60 Norsvin Topigs-20 strain, 22 Gottingen minipigs, 40 Yucatan minipigs. Cell-based assays developed for various AAV serotypes. The tested a Lec-2 cell line AAV9 vector COS-7 other In strain 2 4 years...
Tick-borne encephalitis (TBE) is a viral infection of the CNS caused by TBE virus. With no specific treatment available, only protection formalin-inactivated whole virus vaccine. Primary immunization with European vaccines, as recommended manufacturers, consists three vaccine doses administered within 1-y period. Protection from vaccination believed to be mediated Abs, yet T cells may also have protective role. We set out characterize human CD4+ cell response throughout primary immunization....
Antigen presentation by HLA class I (HLA-I) and II (HLA-II) complexes is achieved proteins that are specific for their respective processing pathway. The invariant chain (Ii)-derived peptide CLIP required HLA-II-mediated antigen stabilizing HLA-II molecules before loading through transient promiscuous binding to different grooves. Here, we demonstrate alternative of surface HLA-I on leukemic cells. In HLA-II-negative AML cells, found plasma membrane display the peptide. Silencing Ii in cells...
HLA-E is a nonclassical HLA class I molecule, which differs from classical molecules by its nonpolymorphic, conserved nature. Expression and function of in normal tissues solid tumors not fully understood. We investigated protein expression on tissue sections 420 ovarian cervical cancers found equal or higher levels than counterpart epithelia 80% the tumors. was strongly associated with components antigen presentation pathway, e.g., transporter processing (TAP), endoplasmic reticulum...