A5046409946- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Transplantation: Methods and Outcomes
- Polyomavirus and related diseases
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Renal Transplantation Outcomes and Treatments
- Neuroscience and Neuropharmacology Research
- Virus-based gene therapy research
- Ion channel regulation and function
- Adipokines, Inflammation, and Metabolic Diseases
- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Neurotransmitter Receptor Influence on Behavior
- Corneal Surgery and Treatments
- Prostate Cancer Treatment and Research
- Pancreatic function and diabetes
- Prenatal Screening and Diagnostics
- Schizophrenia research and treatment
- Acute Myeloid Leukemia Research
- Cardiovascular Disease and Adiposity
- Immunodeficiency and Autoimmune Disorders
Karolinska Institutet
2009-2019
Karolinska University Hospital
2009-2017
Integrated Cardio Metabolic Centre
2015
Linköping University
2014
Industrial Development Center (Sweden)
2010
We analyzed the outcome of allogeneic hematopoietic stem cell transplantation (HSCT) over past 2 decades. Between 1992 and 2009, 953 patients were treated with HSCT, mainly for a hematologic malignancy. They divided according to 4 different time periods treatment: 1995, 1996 2000, 2001 2005, 2006 2009. Over years, many factors have changed considerably regarding patient age, diagnosis, disease stage, type donor, source, genomic HLA typing, dose, conditioning, treatment infections, use...
Background. Viral infections are major complications after allogeneic hematopoietic stem cell transplantation (HSCT). During posttransplant immunosuppression the regular T-cell control is compromised. Even if treatment strategies against caused by herpes viruses such as cytomegalovirus, Epstein-Barr virus, and adenovirus have improved, mortality rate still considerable. If primary antiviral therapy fails or cannot be tolerated, adoptive with virus-specific cytotoxic T cells (CTL) can utilized.
Escitalopram, the S-enantiomer of citalopram, possesses superior efficacy compared to other selective serotonin reuptake inhibitors (SSRIs) in treatment major depression. Escitalopram binds an allosteric site on transporter, which further enhances blockade reuptake, whereas R-citalopram antagonizes this positive modulation. Escitalopram's effects neurotransmitters than serotonin, for example, dopamine and glutamate, are not well studied. Therefore, we here studied escitalopram, cell firing...
Long-term stable mixed chimerism (MC) is a rare phenomenon after hematopoietic stem cell transplantation (HSCT) characterized by 5% to 95% residual recipient cells. The underlying mechanisms of MC are largely unknown. In this study we compared full donor with long-lasting for median 9.5 years (range, 5 16.5) post-HSCT in patients nonmalignant diseases. Several factors significantly associated the likelihood development were identified univariate analysis, eg, younger age, sibling donor, and...
The amount of intra-thoracic fat, which mediastinal adipose tissue comprises the major depot, is related to various cardiometabolic risk factors. Autopsy and imaging studies indicate that depot in adult humans could contain brown (BAT). To gain a better understanding this fat we examined possible BAT characteristics human comparison with subcutaneous tissue. Adipose biopsies from thoracic depots were obtained during open-heart surgery 33 subjects (26 male, 63.7±13.8 years, body mass index...
Chronic graft-versus-host disease (cGVHD) is a debilitating complication arising in around half of all patients treated with an allogeneic hematopoietic stem cell transplantation. Even though treatment severe cGVHD has improved during recent years, it remains one the main causes morbidity and mortality affected patients. Biomarkers blood that could aid diagnosis classification severity are needed for development novel strategies can alleviate symptoms reduce need painful sometimes...
Antidepressant drugs are frequently used to treat affective symptoms in schizophrenia. We have recently shown that escitalopram, but not citalopram or R-citalopram, increases firing rate and burst of midbrain dopamine neurons, potentiates cortical N-methyl-D-aspartate (NMDA) receptor-mediated transmission enhances cognition, effects might influence the outcome concomitant antipsychotic medication. Here, we studied, rats, behavioral neurobiological adding citalopram, R-citalopram...
Cord blood (CB) as a source of stem cells has been successful addition to the field allogeneic cell transplantation (ASCT). The increased human leukocyte antigen (HLA) permissiveness CB grafts made it possible for more patients undergo treatment. drawback is that suffer from longer period compromised immunity. We analyzed T-cell receptor excision circles (TRECs), immunoglobulin G (IgG), and M (IgM) levels after cord (CBT) in 50 transplanted at our center. These immunological parameters were...
Allogeneic stem cell transplantation (SCT) from cord blood (CB) as a source is promising alternative when no human leukocyte antigen-matched donor found. Donor lymphocyte infusion (DLI) possible treatment modality for threatening graft failure or relapse of an underlying malignancy after transplantation. Ethical and logistical reasons limit the possibility DLI in setting CB SCT. To remedy this restriction, we performed expansion T cells vitro grafts clinical use future characterized expanded...
Long-term stable mixed chimerism is a rare and poorly understood phenomenon post hematopoietic stem cell transplantation. This study aims to shed light on whether the two systems in patients with remain functional. Additionally, we investigate possible immunologic differences these individuals compared only donor derived immune cells. Patients chimerism, at median 10 (5–16) years post-HSCT for non-malignant diseases, were assessed regarding clinical situation system (phenotypical...
Influenza A virus (IAV) infection constitutes an annual health burden across the globe. Plasmacytoid dendritic cells (PDCs) are central in antiviral defense because of their superior capacity to produce type I IFNs response viruses. Dendritic (DCs) differ depending on anatomical location. However, only limited host-pathogen data available from initial site humans. In this study, we investigated how human tonsil PDCs, likely exposed location, responded IAV compared with peripheral blood PDCs....
Umbilical cord blood transplantation (UCBT) is increasingly used and produces similar results to matched unrelated donor transplantation.We performed a retrospective single-center analysis of 50 umbilical transplantations UCBTs from 2001 2010, including 37 single 13 double UCBTs.The rate engraftment neutrophils was 88% at median time 29 days (range, 3-79). Complete chimerism (DC) within the CD19, CD3, CD33 cell lineages seen in 74%, 72%, 76% patients, respectively. DC associated with acute...
Abstract Introduction Despite routine use of umbilical cord blood ( CB ) grafts as stem cell source for allogeneic transplantations, much remains unknown regarding their composition and correlation with clinical outcome. Methods We analyzed material from 30 units used hematopoietic transplantation by multicolor flow cytometry. Phenotypic data were correlated various outcomes such survival, graft‐versus‐host disease GVHD ), relapse, rejection, viral reactivation, bacteremia. Results found...
Summary Double cord blood transplantation (DCBT) with two matched or partially units has been implemented successfully to circumvent the limitations of graft cell dose associated single CBT. After DCBT, sustained haematopoiesis is derived almost exclusively from only one donated units. None less, we previously observed six evaluable DCBT patients still having mixed donor–donor chimerism at 28 and 45 months post-transplantation, respectively. In present study utilize flow cytometry techniques...
Background . Graft-versus-host disease (GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation (HSCT). We designed functional assay for assessment of individual risk acute GVHD. Study Design and Methods Blood samples were collected from patients donors before HSCT. Two groups seven each selected, one in which individuals developed GVHD grades II–IV none showed any clinical signs Peripheral blood mononuclear cells (PBMCs) isolated incubated mixed lymphocyte...
One disadvantage with umbilical cord blood transplantation is that donor lymphocyte infusion (DLI) for treatment of threatening rejection or relapse underlying malignant disease not available. We have previously expanded T cells from the graft in clinical setting using anti-CD3/CD28 paramagnetic beads and interleukin (IL)-2 possible future DLI. Here we studied effect adding clinical-grade IL-7 to expansion protocol. were positively selected cultured increasing concentrations IL-2 without (20...
Ion channels in the plasma membrane are important for apoptotic process. Different types of voltage-gated ion up-regulated early process and block these prevents or delays apoptosis. In present investigation we examined whether oocytes from frog Xenopus laevis during The two-electrode voltage-clamp technique was used to record endogenous currents oocytes. During staurosporine-induced apoptosis a voltage-dependent Na+ current increased three-fold. This activated at voltages more positive than...
T cells are pivotal in the immune defense against cancers and infectious agents. To mount an effector response cancer cells, need to migrate cancer-site, engage contacts with perform their functions. Adoptive cell therapy is effective strategy as treatment of complications such relapse or opportunistic infections after hematopoietic stem transplantations. This requires a sufficient amount that able expand respond tumor viral antigens. The cytokines interleukin (IL)-2 IL-7 drive...
Double cord blood transplantation has successfully been introduced to remedy the obstacle of a limited stem cell dose in single graft. After short initial period, sustained hematopoiesis is derived almost exclusively from one donated units. In recent publication Clinical and Experimental Immunology we investigated two rare individuals which both units co-existed for more than years after transplantation.
Therapies for localized prostate cancer include curative surgery and radiotherapy while treatment of metastatic disease is often inefficient. Graft-versus-tumor effects allogeneic stem-cell transplantation (ASCT) have been described several types solid tumors but not reported cancer. We, therefore, investigated the potential ASCT as noncurable cancer.A patient underwent from his human leukocyte antigen (HLA)-identical sister adenocarcinoma. Frequencies prostate-specific T cells in peripheral...
Background: When no HLA-identical donor is available, cord blood transplantation (CBT) an attractive option due to the rapid availability of graft and its biological properties. One disadvantages associated with CBT lack possibility for lymphocyte infusion (DLI) after CBT. Here we report expansion characterization CD3 positive lymphocytes from CB grafts in connection Material Methods: Lymphocytes 13 were expanded CD3/CD28 beads 200 IU/ml rIL2. Expanded cells cryopreserved ready-to-use DLI...