A5071093438- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
- Transplantation: Methods and Outcomes
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- Polyomavirus and related diseases
- Blood transfusion and management
- Renal Transplantation Outcomes and Treatments
- Blood donation and transfusion practices
- Prenatal Screening and Diagnostics
- Viral-associated cancers and disorders
- Organ Transplantation Techniques and Outcomes
- Prostate Cancer Treatment and Research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Immunodeficiency and Autoimmune Disorders
- Telomeres, Telomerase, and Senescence
- Erythropoietin and Anemia Treatment
- Corneal Surgery and Treatments
- Lipid Membrane Structure and Behavior
Karolinska Institutet
2016-2025
Karolinska University Hospital
2016-2025
KTH Royal Institute of Technology
2016-2022
Science for Life Laboratory
2017-2022
Institute for Transfusion Medicine
2011
The transcription factor TOX is not exclusively linked to T cell exhaustion (see related Focus by Utzschneider and Kallies).
Allogeneic hematopoietic stem cell transplantation is a successful treatment for hematologic malignancies and variety of genetic metabolic disorders. In the period following transplantation, immune-compromised milieu allows opportunistic pathogens to thrive. Epstein-Barr virus-associated post-transplant lymphoproliferative disease can be life-threatening complication transplanted patients because suppressed T-cell-mediated immunity. We analyzed possible risk factors associated with in cohort...
Abstract Mesenchymal stem cells (MSCs), isolated from adult human bone marrow, have immunomodulatory properties. The functional outcomes of MSCs–CTL interactions remain poorly characterized. In this study, we demonstrate that MSCs resistant to CTL lysis, even after pulsing with the specific synthetic peptide at high concentrations, in spite surface expression relevant MHC class I allele. were also much less sensitive lysis by an allo-specific clone as compared HLA-matched lymphoblastoid cell...
We analyzed the outcome of allogeneic hematopoietic stem cell transplantation (HSCT) over past 2 decades. Between 1992 and 2009, 953 patients were treated with HSCT, mainly for a hematologic malignancy. They divided according to 4 different time periods treatment: 1995, 1996 2000, 2001 2005, 2006 2009. Over years, many factors have changed considerably regarding patient age, diagnosis, disease stage, type donor, source, genomic HLA typing, dose, conditioning, treatment infections, use...
Background. Viral infections are major complications after allogeneic hematopoietic stem cell transplantation (HSCT). During posttransplant immunosuppression the regular T-cell control is compromised. Even if treatment strategies against caused by herpes viruses such as cytomegalovirus, Epstein-Barr virus, and adenovirus have improved, mortality rate still considerable. If primary antiviral therapy fails or cannot be tolerated, adoptive with virus-specific cytotoxic T cells (CTL) can utilized.
During more recent years only few studies have analyzed the effect of total nucleated cell (TNC) and CD34+ dose in allogeneic hematopoietic stem transplantation (HSCT). A single-center analysis included 544 patients, 227 with a sibling donor 317 an unrelated donor. Most patients (n = 292) were treated myeloablative conditioning, whereas remaining 252) received reduced-intensity conditioning. Bone marrow (BM) 121) peripheral blood (PBSC) grafts 423) separately. Median TNC was 3.2 × 108/kg...
Suppression of immune reactivity by increased expression co-inhibitory receptors has been discussed as a major reason to why the system fails control tumor development. Elucidating pattern tumor-infiltrating lymphocytes in different cancer types will help develop future treatment strategies. We characterized markers reflecting and affecting T-cell functionality flow cytometry on isolated from blood, ascites advanced ovarian patients (n = 35). Significantly higher proportions CD4+ CD8+...
γδ T cells are clonotypically distinct in ascites and tumors of patients with ovarian cancer their functionality correlates clinical outcome.
Here, we report a screening platform for assessment of the cytotoxic potential individual natural killer (NK) cells within larger populations. Human primary NK were distributed across silicon-glass microchip containing 32,400 microwells loaded with target cells. Through fluorescence and automated image analysis, numbers live or dead in each well could be assessed at different time points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging quantifying killing...
Abstract Objectives The clinical outcome after allogeneic haematopoietic stem cell transplantation (aHCT) relies greatly on the efficient recovery of T cells. Several studies have investigated short‐term γδ reconstitution and their role in outcomes following transplantation. Nevertheless, long‐term characteristics impact remained largely unknown. Methods We analysed cells from 20 recipient/donor pairs at phenotypic, clonotypic functional levels to assess ≥ 8 years (median 18 years)...
Abstract The study included 110 consecutive patients with hematological malignancies receiving fludarabine‐based reduced intensity conditioning ( RIC ) and hematopoietic stem cell transplantation HSCT from matched unrelated donors. median age was 55 yr (range 11–68) all but 15 received peripheral blood grafts. Antithymocyte globulin ATG (Thymoglobulin, Genzyme) at a total dose of 6 mg/kg (n = 66) or 8 44) given to according protocol. did not affect time‐to‐neutrophil platelet engraftment....
Benign prostatic hyperplasia (BPH) is a common chronic non-malignant condition whose prevalence substantially increases with age. Immune cell infiltration and pro-inflammatory mediators have been implicated in the pathogenesis. Here, we characterized 21 extracellular markers on prostate-infiltrating lymphocytes (PILs) analyzed expression of 26 soluble proteins prostate tissue obtained from BPH patients (n = 31). These data were correlated clinical parameters compared peripheral blood...
Allogeneic hematopoietic stem cell transplantation (HSCT) can be performed across the ABO blood group barrier. The impact of incompatibility on clinical outcome is controversial. A retrospective analysis 310 patients who underwent HSCT with reduced-intensity conditioning between 1998 and 2011 was to investigate frequency implications anti-RBC antibodies in passenger lymphocyte syndrome (PLS) after minor mismatch (mm), persistent or recurring recipient type (PRABO) major mm HSCT, autoimmune...
Allogeneic hematopoietic stem cell transplantation is associated with several complications and risk factors, for example, graft versus host disease (GVHD), viral infections, relapse, rejection. While high levels of CD3+ cells in grafts can contribute to GVHD, they also promote the leukemia (GVL) effect. Infusions extra lymphocytes from original donor be used as a treatment after relapse or poor immune reconstitution but increase GVHD. In peripheral blood, 95% T-cells express the<mml:math...
Long-term stable mixed chimerism (MC) is a rare phenomenon after hematopoietic stem cell transplantation (HSCT) characterized by 5% to 95% residual recipient cells. The underlying mechanisms of MC are largely unknown. In this study we compared full donor with long-lasting for median 9.5 years (range, 5 16.5) post-HSCT in patients nonmalignant diseases. Several factors significantly associated the likelihood development were identified univariate analysis, eg, younger age, sibling donor, and...
Background and objectives Commercial blood bags are predominantly made of polyvinyl chloride (PVC) plasticized with di(2‐ethylhexyl) phthalate (DEHP). DEHP is favourable for storage red cells (RBC). Historically, removal from has been linked to unacceptable haemolysis levels. Oncoming regulatory restrictions due toxicity concerns increase the urgency replace without compromising RBC quality. Di(2‐ethylhexyl) terephthalate (DEHT) one suggested substitute. The aim this study was compare...
Abstract Background Intratumoral injection of oncolytic viruses (OVs) shows promise in immunotherapy: ONCOS-102, a genetically engineered OV that encodes Granulocyte–Macrophage Colony-Stimulating Factor (GM-CSF) demonstrated efficacy early clinical trials, enhancing T cell infiltration tumors. This suggests OVs may boost various forms immunotherapy, including tumor-specific bi-specific antibodies (BsAbs). Methods Our study investigated vitro, how ONCOS-204, variant ONCOS-virus expressing the...
Tumors are considered to be possible targets of immunotherapy using stimulated and expanded cytotoxic T-lymphocytes (CTL). It is important consider the drug-induced effects when chemotherapeutic regimens CTL-mediated planned used in parallel. In this study, we characterized effect 29 frequently agents on activity autologous allogeneic CTLs. We found that treatment CTLs with following drugs: docetaxel, vincristine, chlorambucil, mitomycin C, oxaliplatin, doxorubicin, bleomycin effectively...