We present an easy-to-use, disposable, thermoplastic microwell chip designed to support screening and high-resolution imaging of single-cell behavior in two- three-dimensional (2D 3D) cell cultures. show that the has excellent optical properties provide simple protocols for efficient long-term culture suspension adherent cells, latter grown either as monolayers or hundreds single, uniformly sized spheroids. then demonstrate applicability system analysis by correlating dynamic cytotoxic...

Understanding the complex 3D tumor microenvironment is important in cancer research. This can be modelled vitro by culturing multicellular spheroids (MCTS). Key challenges when using MCTS applications such as high-throughput drug screening are overcoming imaging and analytical issues encountered during functional structural investigations. To address these challenges, we use an ultrasonic standing wave (USW) based culture platform for parallel formation, staining of 100 whole MCTS. A protein...

Here, we report a screening platform for assessment of the cytotoxic potential individual natural killer (NK) cells within larger populations. Human primary NK were distributed across silicon-glass microchip containing 32,400 microwells loaded with target cells. Through fluorescence and automated image analysis, numbers live or dead in each well could be assessed at different time points after initial mixing. Cytotoxicity was also studied by time-lapse live-cell imaging quantifying killing...

A novel, simple and robust route for binary separation of viable dead cells using a density modified medium which takes advantage the compromised cell membrane cells.

Immune cells exhibit functional heterogeneity beyond what is resolved by classical definitions of subpopulations based on cell surface expression receptors. To develop efficient and personalized cell-based immunotherapies, we need to resolve this understand the underlying parameters that dictate cellular responses specific target cells. For this, new methods are required can identify harvest immune with functions, e.g., high cytotoxic potential, form clonally expanded or assess molecular...

The effect of a resonant magnetic perturbation (RMP) on the tearing mode (TM) dynamics is experimentally studied in EXTRAP T2R device. equipped with set sensor coils and active connected by digital controller allowing feedback control instabilities. recently upgraded algorithm allows suppression all error field harmonics but keeping selected harmonic to desired amplitude, therefore opening possibility clear study RMP corresponding TM. paper shows that produces two typical effects: (1) weak...

Acoustic trapping is a promising technique for aligning particles in two-dimensional arrays, as well dynamic manipulation of individually or groups. The actuating principles used current systems rely on either cavity modes enclosures complex arrangements phase control. Therefore, available require high power inputs and costly peripheral equipment sacrifice flexibility. This work presents different concept acoustic cells that enables dynamically defined patterns inside simple inexpensive...

Here, we present a methodology based on multiplexed fluorescence screening of two- or three-dimensional cell cultures in newly designed multichambered microwell chip, allowing direct assessment drug immune cytotoxic efficacy. We establish framework for culture, formation tumor spheroids, labeling, and imaging fixed live cells at various magnifications directly the chip together with data analysis interpretation. The is demonstrated by cytotoxicity using ovarian non-small lung cancer cellular...

Activated dopamine derivatives are synthesized and evaluated as primers between a TEC-crosslinked matrix bone. Dopamine shown to significantly increase the adhesive strength of fiber-reinforced bone patches.

The non-resonant magnetic perturbation (MP) braking is studied in the EXTRAP T2R reversed-field pinch (RFP) and experimental torque compared with expected by neoclassical toroidal viscosity (NTV) theory. active coils can apply perturbations a single harmonic, either resonant or non-resonant. MP produces velocity an that affects large part of core region. clearly related to plasma displacement, consistent quadratic dependence as NTV work show good qualitative agreement between RFP machine...

In cancer research and drug screening, multicellular tumor spheroids (MCTSs) are a popular model to bridge the gap between in vitro vivo. However, current techniques culture mixed co-culture MCTSs do not mimic structural architecture cellular spatial distribution solid tumors. this study we present an acoustic trapping-based core-shell method using sequential seeding of core shell cells into microwells coated with protein repellent coating. Scaffold-free ovarian OVCAR-8 cell line were...

Experiments in the EXTRAP T2R reversed field pinch [P. R. Brunsell, H. Bergsåker, M. Cecconello et al., Plasma Phys. Control. Fusion 43, 1457 (2001)] on feedback control of m=1 resistive wall modes (RWMs) are compared with simulations using cylindrical linear magnetohydrodynamic model, including dynamics active coils and power amplifiers. Stabilization main RWMs (n=−11,−10,−9,−8,+5,+6) is shown modest loop gains order G∼1. However, other marginally unstable (n=−2,−1,+1,+2) driven by external...

The EXTRAP T2R feedback system (active coils, sensor coils and controller) is used to study develop new tools for advanced control of the MHD instabilities in fusion plasmas. New algorithms developed reversed-field pinch allow flexible independent each magnetic harmonic. Methods theory applied a closed-loop identification machine plant resistive wall modes growth rates. starting point development output-tracking which enable generation external perturbations. These will then be effect...

Abstract Multicellular tumor spheroids (MCTSs) can serve as in vitro models for solid tumors and have become widely used basic cancer research drug screening applications. The major challenges when studying MCTSs by optical microscopy are imaging analysis due to light scattering within the 3-dimensional structure. Herein, we an ultrasound-based MCTS culture platform, where A498 renal carcinoma were cultured, DAPI stained, optically cleared imaged, connect nuclear segmentation biological...

The attractive colloidal and physicochemical properties of cellulose nanofibers (CNFs) at interfaces have recently been exploited in the facile production a number environmentally benign materials, e.g. foams, emulsions, capsules. Herein, these unique are new type CNF-stabilized perfluoropentane droplets produced via straightforward simple mixing protocol. Droplets with comparatively narrow size distribution (ca. 1–5 μm diameter) were fabricated, their potential acoustic droplet vaporization...

Recent developments and applications of system identification methods for the reversed-field pinch (RFP) machine EXTRAP T2R have yielded plasma response parameters decoupled dynamics. These data sets are fundamental a real-time implementable fast Fourier transform (FFT) discrete-time fixed-order strongly stabilizing synthesis as described in this work. Robustness is assessed over set by bootstrap calculation sensitivity transfer function worst-case -gain distribution. Output tracking...

The usage of computationally feasible overparametrized and nonregularized system identification signal processing methods is assessed for automated determination the full reversed-field pinch external plasma response spectrum experiment EXTRAP T2R. No assumptions on geometry eigenmodes are imposed. attempted approach consists high-order autoregressive exogenous estimation followed by Markov block coefficient construction Hankel matrix singular value decomposition. It seen that obtained...

The linearized magnetofluid dynamics of a magnetically confined reversed-field pinch plasma is extracted from set perturbative randomized nondestructive experiments. spectrum the generically estimated linear time-invariant system compared to solutions corresponding eigenvalue problems formed by ideal magnetohydrodynamic model. Stable and unstable nonresonant resistive wall modes are accurately identified.

We first briefly summarize a supposedly efficient novel method for measuring the external plasma response as applied to inherently unstable reversed-field pinch EXTRAP T2R. Second, set of parameters estimated with this particular is harvested and fed input discrete-time fixed-order fast-Fourier-transform-decoupled multi-input-multioutput controller synthesis. The thus reconfigured feedback system implemented experimentally tested on real plant recorded first-deployment results are...

A new method for the estimate of wall diffusion time non-axisymmetric fields is developed. The based on rotating external and measurement frequency response developed tested in EXTRAP T2R. allows experimental each Fourier harmonic toroidal asymmetries. intrinsically considers effects three-dimensional structures shell gaps. Far from gaps, results are good agreement with estimated a simple cylindrical model that assumes homogeneous wall. also applied non-standard configurations coil array,...