A5012837232- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Multiple Sclerosis Research Studies
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Antimicrobial Peptides and Activities
- SARS-CoV-2 and COVID-19 Research
- Atherosclerosis and Cardiovascular Diseases
- Protein purification and stability
- Immune Response and Inflammation
- Eosinophilic Esophagitis
- Respiratory and Cough-Related Research
- vaccines and immunoinformatics approaches
- Pediatric health and respiratory diseases
- Drug-Induced Adverse Reactions
- Mast cells and histamine
- Pancreatic function and diabetes
- Sarcoidosis and Beryllium Toxicity Research
- Diabetes and associated disorders
Karolinska Institutet
2015-2025
Karolinska University Hospital
2004-2013
ENT and Allergy
2004-2008
Vienna General Hospital
2004
Hôpitaux Universitaires de Strasbourg
2004
University of Vienna
2004
Pfizer (United States)
1999
Pharmac
1999
1928 Diagnostics (Sweden)
1999
Multiple sclerosis is an autoimmune disease that caused by the interplay of genetic, particularly HLA-DR15 haplotype, and environmental risk factors. How these etiologic factors contribute to generating autoreactive CD4+ T cell repertoire not clear. Here, we demonstrate self-reactivity, defined as "autoproliferation" peripheral Th1 cells, elevated in patients carrying haplotype. Autoproliferation mediated memory B cells a HLA-DR-dependent manner. Depletion vitro therapeutically vivo...
IgE-mediated allergy affects >25% of the population in industrialized countries. Repeated contact with disease-eliciting allergens induces rises allergen-specific IgE Abs and progression disease to more severe manifestations. Our study uses a type vaccine that is based on genetically modified allergen derivatives treat allergic patients. We developed hypoallergenic major birch pollen allergen, Bet v 1, by genetic engineering vaccinated pollen-allergic patients (n = 124) double-blind,...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system, for which and Epstein-Barr virus (EBV) infection a likely prerequisite. Due to homology between nuclear antigen 1 (EBNA1) alpha-crystallin B (CRYAB), we examined antibody reactivity EBNA1 CRYAB peptide libraries in 713 persons with MS (pwMS) 722 matched controls (Con). Antibody response amino acids 7 16 was associated (OR = 2.0), combination high responses positivity markedly increased risk 9.0). Blocking...
Treatment of patients with allergic asthma using low doses peptides containing T cell epitopes from Fel d 1, the major cat allergen, reduces sensitization and improves surrogate markers disease. Here, we demonstrate a key immunological mechanism, linked epitope suppression, associated this therapeutic effect. selected single allergen resulted in suppression responses to other (“linked”) within same molecule. This phenomenon was induced after peptide immunotherapy human asthmatic subjects...
Abstract Patients with I g E antibodies against the carbohydrate epitope galactose‐α‐1,3‐galactose (α‐ G al) have reported severe allergic reactions after consumption of red meat. Investigations revealed associations between to α‐ al and tick bites. We provide first direct evidence that is present within ticks thus potentially explaining relationship exposure sensitization al, development meat allergy as a secondary phenomena. Serum from S wedish patients delayed was included in study. A...
Abstract Background House dust mites (HDM) Dermatophagoides pteronyssinus are a frequent indoor allergen source. Our aim was to determine the frequencies of IgE reactivity purified HDM molecules in mite allergic patients from different parts Europe order establish an panel for diagnosis allergy. Materials and methods Populations D. ‐allergic Austria ( n = 56), France 55), Italy 67) Sweden 65) storage 31) were analysed eight natural (n) recombinant (r) allergens (nDer p 1, rDer 2, nDer 4, 5,...
Significance Despite the growing evidence that autoantibodies are team players in pathogenesis of multiple sclerosis (MS), target autoantigens yet to be identified. In this work, we mined autoantibody repertoire within MS by screening more than 2,000 plasma samples from patients with and controls identified increased reactivity against an ion-channel protein called “anoctamin 2” (ANO2). This finding points toward ANO2 autoimmune sub-phenotype might contribute development clinical algorithms...
Abstract Background Allergy to cats and dogs polysensitization towards these animals are associated with severe childhood asthma. Molecular‐based allergy diagnostics offers new opportunities for improved characterization has been suggested be particularly useful in patients and/or The aim was use extract‐ molecular‐based compare patterns of IgE sensitization aeroallergens children problematic controlled Methods Children a positive ImmunoCAP any furry animal (cat, dog or horse) were recruited...
Abstract Background Allergy to the domestic dog ( C anis familiaris ) affects 5–10% of population in affluent countries. Three four patients are allergic more than one pet, which can only partially be explained by cross‐reactivity between serum albumins. The lipocalin protein family harbours allergens mammalian species. Methods We set out clone and characterize a novel allergen, investigate its potential role cross‐sensitization dog, cat horse. gene encoding an f 6 was amplified from skin...
Summary Background House dust mites (HDM) are well‐known as a source of indoor aeroallergens and for causing allergic airway diseases. Some proteolytic HDM allergens known to activate respiratory epithelial cells produce pro‐inflammatory mediators, while there is limited knowledge regarding such activity among non‐proteolytic allergens. Objective To investigate whether Der p 2, major allergen Dermatophagoides pteronyssinus , activates mediators involved in asthma pathogenesis elucidate the...
Allergic responses can be triggered by structurally diverse allergens. Most allergens are proteins, yet extensive research has not revealed how they initiate the allergic response and why myriad of other inhaled proteins do not. Among these allergens, cat secretoglobulin protein Fel d 1 is a major allergen responsible for severe responses. In this study, we show that similar to mite dust Der p 2, substantially enhances signaling through innate receptors TLR4 TLR2. contrast however, does act...
Abstract Background: Component‐resolved allergy diagnostics enables the detection of crossreactive or species‐specific allergen components. This study analysed Immunoglobulin E ( I g ) profiles to single components in relation bronchial inflammation severe childhood asthma. Methods: Ninety‐five schoolchildren were assessed, 39 with controlled mild‐to‐moderate asthma and 56 uncontrolled asthmatics. Allergen n = 111) food allergens, pollen perennial aeroallergens using an immunosolid‐phase...
Scope The aim of this work was to investigate the effects high‐intensity ultrasound (sonication), on structure and allergenicity major cow's milk allergen, beta‐lactoglobulin ( BLG ). Methods results Structural changes upon sonication were monitored by circular dichroism spectroscopy, tryptophan emission fluorescence, hydrophobic dye retinol binding, as well digestibility phenol‐oxidase cross‐linking capacity. Allergenicity in individual patients’ sera, basophil activation test, skin prick...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS), in which pathological T cells, likely autoimmune, play a key role. Despite its importance, autoantigen repertoire remains largely uncharacterized. Using novel vitro antigen delivery method combined with Human Protein Atlas library, we screened for cell autoreactivity against 63 CNS-expressed proteins. We identified four previously unreported autoantigens MS: fatty acid-binding protein 7, prokineticin-2,...
The domestic cat (Felis domesticus) is one of the most important causes allergic asthma worldwide. dominating allergen, Fel d 1, composed two heterodimers. Recently, it has been shown that recombinant consisting chain 2 and 1 fused together without additional linker, immunological properties indistinguishable from natural heterodimeric protein. Herein, we report crystal structure monomeric at 1.85-A resolution, determined by multi-wavelength anomalous diffraction using selenomethionine...