A5043100124- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Protein purification and stability
- T-cell and B-cell Immunology
- RNA and protein synthesis mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Escherichia coli research studies
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Vector-borne infectious diseases
- Protease and Inhibitor Mechanisms
- Photosynthetic Processes and Mechanisms
- Biotin and Related Studies
- Legume Nitrogen Fixing Symbiosis
- Peptidase Inhibition and Analysis
- Plant nutrient uptake and metabolism
- Food Allergy and Anaphylaxis Research
- vaccines and immunoinformatics approaches
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Biochemical Acid Research Studies
- HIV/AIDS drug development and treatment
- Galectins and Cancer Biology
Karolinska Institutet
1978-2025
King's College Hospital
2017
Swedish Orphan Biovitrum (Sweden)
2005
Mayo Clinic
2004
University of Oslo
1990-1994
Heinrich Heine University Düsseldorf
1993
Djurkliniken Roslagstull
1970-1992
KTH Royal Institute of Technology
1986-1991
Stockholm County Council
1990
University of Iowa
1989
A synthetic IgG-binding domain based on staphylococcal protein was designed with the aid of sequence comparisons and computer graphic analysis. strategy, utilizing non-palindromic restriction sites, used to overcome difficulties introducing site-specific changes into repetitive gene. single mutagenized gene fragment polymerized different multiplicities, products were expressed in Escherichia coli. Using this scheme, A-like proteins composed numbers domains produced. These changed lack...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system, for which and Epstein-Barr virus (EBV) infection a likely prerequisite. Due to homology between nuclear antigen 1 (EBNA1) alpha-crystallin B (CRYAB), we examined antibody reactivity EBNA1 CRYAB peptide libraries in 713 persons with MS (pwMS) 722 matched controls (Con). Antibody response amino acids 7 16 was associated (OR = 2.0), combination high responses positivity markedly increased risk 9.0). Blocking...
Significance Despite the growing evidence that autoantibodies are team players in pathogenesis of multiple sclerosis (MS), target autoantigens yet to be identified. In this work, we mined autoantibody repertoire within MS by screening more than 2,000 plasma samples from patients with and controls identified increased reactivity against an ion-channel protein called “anoctamin 2” (ANO2). This finding points toward ANO2 autoimmune sub-phenotype might contribute development clinical algorithms...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExpression of human insulin-like growth factor I in bacteria: use optimized gene fusion vectors to facilitate protein purificationTomas Moks, Lars Abrahmsen, Erik Holmgren, Margareta Bilich, Anders Olsson, Gunnar Pohl, Catharina Sterky, Hans Hultberg, Staffan Josephson, and Cite this: Biochemistry 1987, 26, 17, 5239–5244Publication Date (Print):August 25, 1987Publication History Published online1 May 2002Published inissue 25 August...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS), in which pathological T cells, likely autoimmune, play a key role. Despite its importance, autoantigen repertoire remains largely uncharacterized. Using novel vitro antigen delivery method combined with Human Protein Atlas library, we screened for cell autoreactivity against 63 CNS-expressed proteins. We identified four previously unreported autoantigens MS: fatty acid-binding protein 7, prokineticin-2,...
A novel approach for production of small polypeptides, using a staphylococcal protein vector, is described. This system used to express, secrete and purify human insulin-like growth factor I (IGF-I). fusion consisting IGF-I recovered in high yield by passing the culture medium through an IgG affinity column. Using site-specific mutagenesis acid labile asp-pro cleavage site was introduced at point between two proteins. The “tail” can thereby be removed from purified chemical releasing...
Lactic acid bacteria, such as Lactiplantibacillus plantarum, are becoming increasingly popular hosts for combining production and delivery of therapeutic proteins to immune cells. Soluble antigens susceptible rapid proteolysis, hence anchoring bacterial cells, which likely protects the antigen, is a preferred strategy that may increase responses. In cancer research, personalized immunotherapy has high potential and, in this respect, so-called neoantigens accumulate tumor cells promising...
A dual affinity fusion concept has been developed in which the gene encoding desired product is fused between two flanking heterologous genes IgG- and albumin-binding domains. Using sequential IgG serum albumin chromatography, a full-length tripartite protein obtained. This approach was used to recover Escherichia coli containing human insulin-like growth factor II (IGF-II). Surprisingly, recombinant IGF-II showed increased stability against proteolytic degradation E. when produced as...
Tick-borne encephalitis (TBE) has occurred regularly in Sweden since it was first diagnosed 1954. In Austria, where TBE is common, vaccination been shown to reduce the incidence considerably. Since this vaccine now available we analysed 1116 cases of from 1956 October 1989 order define a strategy for vaccination. The were identified records all infectious disease departments complemented with data virological laboratories. All diagnoses confirmed by positive serological result. analysis...
We have isolated a cDNA sequence coding for part of human tissue plasminogen activator. mRNA activator was partially purified, copied into double-stranded cDNA, and cloned Escherichia coli. Two sets overlapping oligodeoxynucleotide mixtures corresponding to all possible sequences known portion the gene were prepared. One set used as probe screen containing bacterial clones both probes in hybridization against purified plasmid DNA. Of 4,200 examined, 1 carried that hybridized...
A succinic dehydrogenase (SDH) complex has been purified from Triton X-100-solubilized membranes Bacillus subtilis by precipitation with specific antibody. Radioactively labeled precipitated was analyzed sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed autoradiography of the gels. The contained equimolar amounts three polypeptides approximate molecular weights 65,000, 28,000, and 19,000. Five dehydrogenase-negative mutants, belonging to citF group, 65,000-dalton polypeptide...
We determined the DNA sequence of Rhizobium meliloti gene encoding anthranilate synthase, first enzyme tryptophan pathway. Sequences similar to those seen for two subunits as found in all other procaryotic species studied are present a single open reading frame 729 codons. This apparent fusion joins C terminus large subunit (TrpE) N small (TrpG) through short connecting segment. designate fused trpE(G). The is flanked by typical rho-independent terminator at 3' end and complex regulatory...
A 5-aminolevulinic acid-requiring mutant of Bacillus subtilis was isolated. When the is shifted from medium containing acid to lacking this growth factor, bacteria continued grow at undiminished rate for about three generations. The membranes these contained severely reduced amounts cytochrome. used study role heme synthesis on and membrane binding succinic dehydrogenase (SDH). amount SDH in whole-cell lysates soluble cytoplasmic fraction determined by one-dimensional (rocket)...
Five to ten percent of the population in affluent countries are allergic dog. Diagnosis and treatment is based on allergen extracts from natural sources where composition concentration poorly defined.We aimed quantify six dog allergens (Can f 1-6) commercial skin prick test (SPT) solutions determine individual profiles dogs.The content SPT five vendors source material three anatomical sites were analyzed. Fur saliva samples collected a mixed 120 dogs. Can 1-6 quantified by inhibition ELISA...
Eleven succinic dehydrogenase (SDH) mutants in Bacillus subtilis were analyzed by crossed immunoelectrophoresis with antiserum prepared against wild-type B. cytoplasmic membrane. A precipitate which stained for SDH was found Triton X-100-solubilized membranes and from two of the mutants. The remaining nine did not show an SDH-staining precipitate. respective mutations these all map one locus, citF (Ohné et al., J. Bacteriol. 115:738-745, 1973). An SDH-specific immunizing rabbits obtained...
Background Dog dander extract used for diagnosis and allergen-specific immunotherapy is often of variable poor quality. Objective To assemble four well-established dog allergen components into one recombinant folded protein improved vaccination allergy to dog. Methods A linked molecule, comprising the lipocalin allergens Can f 1, 2, 4 6 was constructed. The tetrameric structurally characterized by small angle X-ray scattering, compared with each single or an equimolar mix analytical size...
Human parathyroid hormone (hPTH) is a peptide consisting of 84 amino acids (hPTH(1-84)). Employing the promoter and signal sequence Staphylococcus aureus-protein A we have expressed hPTH in Escherichia coli. The proteins are excreted to growth medium, allowing for rapid easy purification desired products. By acid analysis mass spectrometry, shown that major product correctly processed human identical hPTH(1-84). purified recombinant hPTH(1-84) stimulates adenylate cyclase activity rat...