Lactobacillus plantarum is a normal, potentially probiotic, inhabitant of the human gastrointestinal (GI) tract. The bacterium has great potential as food-grade cell factory and for in situ delivery biomolecules. Since protein secretion important both probiotic activity biotechnological applications, we have carried out genome-wide experimental study signal peptide (SP) functionality.We constructed library 76 Sec-type peptides from L. WCFS1 that were predicted to be cleaved by peptidase I....

The probiotic lactic acid bacterium Lactobacillus plantarum is a potential delivery vehicle for mucosal vaccines because of its generally regarded as safe (GRAS) status and ability to persist at the surfaces human intestine. However, inherent immunogenicity vaccine antigens in many cases insufficient elicit an efficient immune response, implying that additional adjuvants are needed enhance antigen immunogenicity. goal present study was increase proinflammatory properties L. by expressing...

ABSTRACT Lactiplantibacillus plantarum, a natural inhabitant of the human body, is promising candidate vehicle for vaccine delivery. An obstacle in developing bacterial delivery vehicles generating production strain that lacks antibiotic resistance genes and contains minimal foreign DNA. To deal with this obstacle, we have constructed finetuned, inducible two-plasmid CRISPR/Cas9-system chromosomal gene insertion L. plantarum . The knock-in plasmid was designed cassette-like structure to...

Lactic acid bacteria, such as Lactiplantibacillus plantarum, are becoming increasingly popular hosts for combining production and delivery of therapeutic proteins to immune cells. Soluble antigens susceptible rapid proteolysis, hence anchoring bacterial cells, which likely protects the antigen, is a preferred strategy that may increase responses. In cancer research, personalized immunotherapy has high potential and, in this respect, so-called neoantigens accumulate tumor cells promising...

Tuberculosis (TB) remains among the most deadly diseases in world. The only available vaccine against tuberculosis is bacille Calmette-Guérin (BCG) vaccine, which does not ensure full protection adults. There a global urgency for development of an effective preventing disease transmission, and it requires novel approaches. We are exploring use lactic acid bacteria (LAB) as vector antigen delivery to mucosal sites. Here, we demonstrate successful expression surface display Mycobacterium...

Abstract Background Utilization of commensal bacteria for delivery medicinal proteins, such as vaccine antigens, is an emerging strategy. Here, we describe two novel food-grade strains lactic acid bacteria, Lactiplantibacillus pentosus KW1 and KW2, well newly developed tools using this relatively unexplored but promising bacterial species production surface-display heterologous proteins. Results Whole genome sequencing was performed to investigate genomic features both identify native...

Chemokines are attractive candidates for vaccine adjuvants due to their ability recruit the immune cells. Lactic acid bacteria (LAB)-based delivery vehicles have potential be used as a cheap and safe option vaccination. Chemokine produced on surface of LAB may potentially enhance response an antigen this approach can considered in development future mucosal vaccines.We constructed strains Lactobacillus plantarum displaying chemokine surface. L. was genetically engineered express anchor...

Mucosal immunity is important for the protection against a wide variety of pathogens. Traditional vaccines administered via parenteral routes induce strong systemic immunity, but they often fail to generate mucosal IgA. In contrast, bacteria-based comprise an appealing strategy antigen delivery sites. Vaginal infection with Chlamydia trachomatis can develop into upper genital tract infections that lead infertility. Therefore, development effective vaccine high priority. present study, we...

Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage healthy C57BL/6 mice. After one month splenocytes were isolated, analysed by flow cytometry. The magnitude splenocyte proliferation upon stimulation with lipopolysaccharide peptidoglycan cytokine levels (IFN-γ, IL-6, IL-10 IL-17) assessed. Cytokine...

Vaccination is considered the most effective strategy for controlling tuberculosis (TB). The existing vaccine, Bacille Calmette-Guérin (BCG), although partially protective, has a number of limitations. Therefore, there need developing new TB vaccines and several strategies are currently exploited including use viral bacterial delivery vectors. We have previously shown that Lactobacillus plantarum (Lp) producing Ag85B ESAT-6 antigens fused to dendritic cell-targeting peptide (referred as...

Given their safe use in humans and inherent adjuvanticity, Lactic Acid Bacteria may offer several advantages over other mucosal delivery strategies for cancer vaccines. The objective of this study is to evaluate the immune responses mice after oral immunization with Lactobacillus (L) plantarum WCFS1 expressing a cell-wall anchored tumor antigen NY-ESO-1. And investigate immunostimulatory potency new candidate vaccine on human dendritic cells (DCs). L. displaying NY-ESO-1 induced specific...

Lactobacillus spp. comprise a large group of Gram-positive lactic acid bacteria with varying physiological, ecological and immunomodulatory properties that are widely exploited by mankind, primarily in food production as health-promoting probiotics. Recent years have shown increased interest using lactobacilli for delivery vaccines, mainly due to their ability skew the immune system towards pro-inflammatory responses. We compared potential eight species, L. plantarum, brevis, curvatus,...

Abstract Members of the genus Lactobacillus have a long history in food applications and are considered as promising safe hosts for delivery medically interesting proteins. We assessed multiple surface anchors derived from plantarum protein display species, using Mycobacterium tuberculosis hybrid antigen test protein. The tested were lipoprotein anchor two cell wall anchors, one non-covalent (LysM domain) covalent (sortase-based anchoring LPXTG motif). Thus, three different expression...

The leaderless bacteriocin Garvicin KS (GarKS) is a potent antimicrobial, being active against wide range of important pathogens. GarKS production by the native producer Lactococcus garvieae KS1546 is, however, relatively low (80 BU/ml) under standard laboratory growth conditions (batch culture in GM17 at 30°C). To improve production, we systematically evaluated impact different media and components on production. Based outcomes, new medium formulation was made that increased about 60-fold...

Lysin motif (LysM) domains are found in many bacterial peptidoglycan hydrolases. They can bind non-covalently to and have been employed display heterologous proteins on the cell surface. In this study, we aimed use a single LysM domain derived from putative extracellular transglycosylase Lp_3014 of Lactobacillus plantarum WCFS1 two different lactobacillal β-galactosidases, heterodimeric LacLM-type reuteri homodimeric LacZ-type delbrueckii subsp. bulgaricus, surface spp. The β-galactosidases...

The bacille Calmette-Guèrin (BCG) vaccine has been used for a century; nonetheless, tuberculosis (TB) remains one of the deadliest diseases in world. Thus, new approaches to developing new, more efficient are desirable. Mucosal vaccines particular interest, considering that Mycobacterium first enters body through mucosal membranes. We have previously demonstrated immunogenicity recombinant Lactiplantibacillus plantarum delivery vector with TB hybrid antigen Ag85B-ESAT-6 anchored cell...

Brown seaweeds are rich in carbohydrates and may be used as a source of fermentable sugars. Saccharification the seaweed biomass can carried out enzymatically by combination cellulases alginate lyases. In this study, thermotolerant exo- endo lyases were cloned expressed Bacillus subtilis. The secreted to culture supernatant directly together with commercial cellulase preparation saccharify Saccharina latissima biomass. results showed that strategy using supernatants worked very well,...

Aims: To exploit promoters involved in production of the bacteriocin sakacin P for regulated overexpression genes Lactobacillus plantarum C11. Methods and Results: Production by Lact. sakei LTH673 is controlled a peptide-based quorum sensing system that drives strong, promoters. One these (PorfX) was used to establish encoding chloramphenicol acetyltransferase from Bacillus pumilus, aminopeptidase N Lactococcus lactis or chitinase B Serratia marcescens C11, strain naturally possesses...

The goal of this work was to elucidate similarities between microorganisms from the perspective humoral immune system reactivity in professional athletes. serum IgG 14 young, individuals analyzed 23 selected as antigens by use house ELISA. Serum IgM and IgA also a control group sex age matched used for comparison. obtained absorbance levels were string values correlate different microorganisms. found be most cross reactive antibody class, Pearson's r = 0.7–0.92, very distant bacterial...

One of the ABC transporter systems in Enterococcus faecalis V583 is encoded by ef0176-ef0180 gene cluster, which differs from orthologous operons related bacteria that it contains two genes putatively encoding substrate-binding proteins (SBPs). These SBPs, EF0176 and EF0177, have previously been identified on surface E. faecalis. By phenotypic studies single double knockout mutants, we show here EF0177 are specific for ribonucleosides and, inference, EF0176-EF0180 plays a role nucleoside...