A5075146226- Invertebrate Immune Response Mechanisms
- Advanced biosensing and bioanalysis techniques
- Insect Utilization and Effects
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- Legume Nitrogen Fixing Symbiosis
- Gold and Silver Nanoparticles Synthesis and Applications
- Microfluidic and Bio-sensing Technologies
- Antimicrobial Peptides and Activities
- Plant nutrient uptake and metabolism
- Optical Imaging and Spectroscopy Techniques
- Insect symbiosis and bacterial influences
- Monoclonal and Polyclonal Antibodies Research
- Photoacoustic and Ultrasonic Imaging
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Meat and Animal Product Quality
- Mosquito-borne diseases and control
- Autophagy in Disease and Therapy
- Food Quality and Safety Studies
- Nanofabrication and Lithography Techniques
- Infrared Thermography in Medicine
- Electrowetting and Microfluidic Technologies
Korea Electrotechnology Research Institute
2012-2025
Chonnam National University
2019-2021
National Research Institute of Fire and Disaster
2021
Ministry of Food and Drug Safety
2018
Chung-Ang University
2014-2015
Korea University of Science and Technology
2012
Seoul National University
1993-2009
Sogang University
2003-2005
Harvard University
1994
University of Iowa
1989-1991
We investigated the use of thermally treated polydimethylsiloxane (PDMS) for chemically-resistant microchannels. When PDMS underwent thermal treatment at 300 °C, swelling was reduced and surface microfluidic channel endured well in extracting media such as dichloromethane. Furthermore, despite small decrease size after treatment, both shape transparency were maintained without showing fluid leakage. The had more hydrophilic properties compared to untreated PDMS. A single step post-casting...
Diffuse optical tomography (DOT) has been investigated as an alternative imaging modality for breast cancer detection thanks to its excellent contrast hemoglobin oxidization level. However, due the complicated non-linear photon scattering physics and ill-posedness, conventional reconstruction algorithms are sensitive parameters such boundary conditions. To address this, here we propose a novel deep learning approach that learns obtains accurate three dimensional (3D) distribution of...
We determined the DNA sequence of Rhizobium meliloti gene encoding anthranilate synthase, first enzyme tryptophan pathway. Sequences similar to those seen for two subunits as found in all other procaryotic species studied are present a single open reading frame 729 codons. This apparent fusion joins C terminus large subunit (TrpE) N small (TrpG) through short connecting segment. designate fused trpE(G). The is flanked by typical rho-independent terminator at 3' end and complex regulatory...
Human clonorchiasis, caused by infection with the trematode Clonorchis sinensis, is a common health problem in East Asia. In an attempt to develop new, sensitive method for diagnosis of disease, use real-time PCR (targeting internal-transcribed-spacer-2 sequence parasite) detect C. sinensis-specific DNA faecal samples has recently been evaluated. The PCR-based assay, which included internal control any inhibition amplification constituents sample, was performed on stool and controls...
Diffuse optical tomography (DOT) non-invasively measures the functional characteristics of breast lesions using near infrared light to probe tissue properties. This study aimed evaluate a new digital tomosynthesis (DBT)/DOT fusion imaging technique and obtain preliminary data for cancer detection. Twenty-eight women were prospectively enrolled underwent both DBT DOT examinations. DBT/DOT was created after acquisition Two radiologists analyzed images independently, then finally evaluated...
Abstract Immune deficiency (IMD) is a death domain-containing protein that essential for the IMD/NF-κB humoral and epithelial immune responses to Gram-negative bacteria viruses in insects. In signaling cascade, IMD recruited together with FADD caspase DREDD after mobilization of PGRP receptors. Activated regulates expression effector antimicrobial peptides (AMP) protect against invading microorganisms. To date, most studies pathway, gene particular, have been restricted Drosophila; few...
Spätzle is an extracellular protein that activates the Toll receptor during embryogenesis and immune responses in Drosophila. However, functions of spätzle proteins innate response against bacteria or fungi T. molitor are not well understood. Therefore, this study, open reading frame (ORF) TmSpz6 was identified its function to bacterial fungal infections investigated using RNAi. The highest expression prepupae, 3- 6-day-old pupae, while remarkable also observed other stages. tissue-specific...
An immunosensor for the detection of pathogens was developed using imaging ellipsometry (IE) as a method. Yersinia enterocolitica selected target pathogen in this study. A gold surface deposited with self-assembled layer 11-mercaptoundecanoic acid (11-MUA) used substrate. For fabrication immunosensor, protein G spots were made on substrate an inkjet-type microarrayer, and monoclonal antibody (Mab) adsorbed onto spots. Deposition each confirmed by measurement plasmon resonance. The...
A colorimetric sensor array was developed to characterize and quantify the taste of white wines. charge-coupled device (CCD) camera captured images from 23 different wine samples, change in R, G, B color components control were analyzed by principal component analysis. Additionally, high performance liquid chromatography (HPLC) used analyze chemical each sample responsible for its taste. two-dimensional score plot created with data points. It revealed clusters same type grape, trends...
Although it is known that the Drosophila Toll-7 receptor plays a critical role in antiviral autophagy, its function other insects has not yet been reported. Here, we have identified Toll-like 7 gene, TmToll-7, coleopteran insect T. molitor and examined potential antibacterial antifungal immunity. We showed TmToll-7 expression was significantly induced larvae 6 h after infection with Escherichia coli Staphylococcus aureus 9 Candida albicans. However, even though by all three pathogens, found...
Spätzle family proteins activate the Toll pathway and induce antimicrobial peptide (AMP) production against microbial infections. However, functional importance of Tmspätzle4 (TmSpz4) in immune response Tenebrio molitor has not been reported. Therefore, here, we have identified functionally characterized role TmSpz4 bacterial fungal We showed that expression was significantly induced hemocytes at 6 h post-injection with Escherichia coli, Staphylococcus aureus, Candida albicans. knock-down...
Aim In this study, the effectiveness of combining each seven types acids with 3% salt as a treatment against pathogens was investigated in laboratory media and acidified food. Methods Results When 0·5% malic, tartaric, citric or 0·25% phosphoric acid combined salt, there higher reduction Gram-negative bacteria (Escherichia coli O157:H7 Salmonella Typhimurium) compared to when using alone. However, acetic, propionic lactic provided protection treatment. antagonistic effects seen were not...
Antimicrobial immune response is mediated by a signal-transducing sensor, peptidoglycan recognition protein-SA (PGRP-SA), that can recognize non-self molecules. Although several studies have focused on the involvement of Drosophila PGRP-SA in antimicrobial peptide (AMP) expression to infections, its role Tenebrio molitor are lacking. Here, we present functional analysis T. (TmPGRP-SA). In absence microbes, TmPGRP-SA was highly expressed late-larval fat body, followed hemocytes, and gut....
The cystine knot protein Spätzle is a Toll receptor ligand that modulates the intracellular signaling cascade involved in nuclear factor kappa B (NF-κB)-mediated regulation of antimicrobial peptide (AMP)-encoding genes. Spätzle-mediated activation pathway critical for innate immune responses insects against Gram-positive bacteria and fungi. In this study, open reading frame (ORF) sequence Spätzle-like from T. molitor (TmSpz-like) identified RNA sequencing dataset was cloned sequenced. 885-bp...
In Rhizobium meliloti, the genes involved in biosynthesis of amino acid tryptophan are found at three separate chromosomal locations. Of gene clusters, trpE(G), trpDC, and trpFBA, only trpE(G) is regulated by end product pathway, tryptophan. We that mRNA contains a leader transcript terminates stem-loop structure putative transcription attenuator. The level this was constant regardless amount growth medium. However, full-length decreased as increased. beta-galactosidase activity an R....