A5103732775- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Immunotherapy and Immune Responses
- Protein Structure and Dynamics
- Asthma and respiratory diseases
- RNA and protein synthesis mechanisms
- T-cell and B-cell Immunology
- Contact Dermatitis and Allergies
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Erythrocyte Function and Pathophysiology
- CAR-T cell therapy research
- Advanced Biosensing Techniques and Applications
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Advanced Electron Microscopy Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Cancer Immunotherapy and Biomarkers
- Glycosylation and Glycoproteins Research
- Hemoglobin structure and function
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Multiple Sclerosis Research Studies
Karolinska University Hospital
2009-2025
Karolinska Institutet
2009-2023
Stockholm University
2013-2020
ENT and Allergy
2011
At what point during translation do proteins fold? It is well established that can fold cotranslationally outside the ribosome exit tunnel, whereas studies of folding inside tunnel have so far detected only formation helical secondary structure and collapsed or partially structured intermediates. Here, using a combination cotranslational nascent chain force measurements, inter-subunit fluorescence resonance energy transfer on single translating ribosomes, molecular dynamics simulations,...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system, for which and Epstein-Barr virus (EBV) infection a likely prerequisite. Due to homology between nuclear antigen 1 (EBNA1) alpha-crystallin B (CRYAB), we examined antibody reactivity EBNA1 CRYAB peptide libraries in 713 persons with MS (pwMS) 722 matched controls (Con). Antibody response amino acids 7 16 was associated (OR = 2.0), combination high responses positivity markedly increased risk 9.0). Blocking...
Abstract Background Allergy to the domestic dog ( C anis familiaris ) affects 5–10% of population in affluent countries. Three four patients are allergic more than one pet, which can only partially be explained by cross‐reactivity between serum albumins. The lipocalin protein family harbours allergens mammalian species. Methods We set out clone and characterize a novel allergen, investigate its potential role cross‐sensitization dog, cat horse. gene encoding an f 6 was amplified from skin...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS), in which pathological T cells, likely autoimmune, play a key role. Despite its importance, autoantigen repertoire remains largely uncharacterized. Using novel vitro antigen delivery method combined with Human Protein Atlas library, we screened for cell autoreactivity against 63 CNS-expressed proteins. We identified four previously unreported autoantigens MS: fatty acid-binding protein 7, prokineticin-2,...
Background Tumour-infiltrating T cells can mediate both antitumour immunity and promote tumour progression by creating an immunosuppressive environment. This dual role is especially relevant in hepatocellular carcinoma (HCC), characterised a unique microenvironment limited success with current immunotherapy. Objective We evaluated cell responses patients advanced HCC analysing tumours, liver flushes liver-draining lymph nodes, to understand whether reactive populations could be identified...
Significance In living cells, proteins are produced in a sequential way by ribosomes. This vectorial process allows the growing protein chain to start fold before translation has been completed. Thereby, cotranslational folding can be significantly different than of full-length isolation. Here, we show how structurally similar repeat domains, normally as parts single long polypeptide, affect their neighbors. provides insight into cell may efficiently produce multidomain proteins, paving for...
Five to ten percent of the population in affluent countries are allergic dog. Diagnosis and treatment is based on allergen extracts from natural sources where composition concentration poorly defined.We aimed quantify six dog allergens (Can f 1-6) commercial skin prick test (SPT) solutions determine individual profiles dogs.The content SPT five vendors source material three anatomical sites were analyzed. Fur saliva samples collected a mixed 120 dogs. Can 1-6 quantified by inhibition ELISA...
<i>Background:</i> Allergen-specific immunotherapy (SIT) is currently the only curative treatment for allergy but needs to be improved. We hypothesize that covalent coupling of immunomodulating vitamin D3 major cat allergen Fel d 1 can enhance beneficial effects SIT allergy. <i>Methods:</i> treated mice sensitized with subcutaneous injections two doses recombinant coupled 1α,25-dihydroxyvitamin (rFel 1:VD3) and compared same rFel in a mouse model Airway...
Allergy and asthma to cat (Felis domesticus) affects about 10% of the population in affluent countries. Immediate allergic symptoms are primarily mediated via IgE antibodies binding B cell epitopes, whereas late phase inflammatory reactions activated T recognition allergen-specific epitopes. Allergen-specific immunotherapy relieves is only treatment inducing a long-lasting protection by induction protective immune responses. The aim this study was produce an allergy vaccine designed with...
Background Dog dander extract used for diagnosis and allergen-specific immunotherapy is often of variable poor quality. Objective To assemble four well-established dog allergen components into one recombinant folded protein improved vaccination allergy to dog. Methods A linked molecule, comprising the lipocalin allergens Can f 1, 2, 4 6 was constructed. The tetrameric structurally characterized by small angle X-ray scattering, compared with each single or an equimolar mix analytical size...
To cite this article : Konradsen JR, Nordlund B, Nilsson OB, van Hage M, Nopp A, Hedlin G, Grönlund H. High basophil allergen sensitivity (CD‐sens) is associated with severe allergic asthma in children. Pediatr Allergy Immunol 2012: 23 376–384. Abstract Children problematic (PA) have persistent symptoms and/or exacerbations despite treatment several drugs. Classification of severity currently based on level and assessment control, but objective biomarkers are needed. investigate the clinical...
Multiple sclerosis (MS) treatment options have improved significantly over the past decades, but consequences of MS can still be devastating and needs for monitoring surveillance are considerable. In current study we used affinity proteomics technology to identify potential biomarkers which could ultimately as facilitate decisions. We profiled intra-individual changes in levels 59 target proteins using an antibody suspension bead array serial plasma samples from 44 patients during with...
Graphical abstractAbstractReliable and sensitive detection of antigen specific cells is essential in several fields research, whether it concerns monitoring responses to infectious agents or exploring the auto-antigen repertoire autoimmune diseases. Identification these however difficult, especially when often are rare methods not sensitive, practical enough. We propose a novel method processing antigens before stimulation which consists covalently binding protein superparamagnetic...
For the past decade, adoptive cell therapy including tumor-infiltrating lymphocytes, genetically modified cytotoxic lymphocytes expressing a chimeric antigen receptor, or novel T-cell receptor has revolutionized treatment of many cancers. Progress within exome sequencing and neoantigen prediction technologies provides opportunities for further development personalized immunotherapies. In this study, we present strategy to deliver in silico predicted neoantigens autologous dendritic cells...
The allergen Can f 2 from dog (Canis familiaris) present in saliva, dander and fur is an important cause of allergic sensitization worldwide. Here, the production, isolation, crystallization preliminary X-ray diffraction analysis two crystal forms recombinant are reported. first form belonged to space group C222, with unit-cell parameters a = 68.7, b 77.3, c 65.1 Å, diffracted 1.55 Å resolution, while second C2, 75.7, 48.3, 68.7 β 126.5°, 2.1 resolution. Preliminary data indicated presence...
Allergy and asthma to cat (Felis domesticus) affects about 10% of the population in affluent countries.Immediate allergic symptoms are primarily mediated via IgE antibodies binding B cell epitopes, whereas late phase inflammatory reactions activated T recognition allergen-specific epitopes.Allergen-specific immunotherapy relieves is only treatment inducing a long-lasting protection by induction protective immune responses.The aim this study was produce an allergy vaccine designed with...
Abstract Proteins synthesized in the cell can begin to fold during translation before entire polypeptide has been produced, which may be particularly relevant folding of multidomain proteins. Here, we study cotranslational adjacent domains from cytoskeletal protein α-spectrin using Force Profile Analysis (FPA). Specifically, investigate how behavior R15 and R16 are affected by their neighboring R14 R16, R17 domains, respectively. Our results show that impact each other’s distinct ways...