Johan Botling
A5091504498- Lung Cancer Treatments and Mutations
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Lung Cancer Research Studies
- Gene expression and cancer classification
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Immunotherapy and Immune Responses
- Hippo pathway signaling and YAP/TAZ
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
- Neuroendocrine Tumor Research Advances
- Lung Cancer Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- Neuroblastoma Research and Treatments
- Cancer-related gene regulation
- Fibroblast Growth Factor Research
- Glycosylation and Glycoproteins Research
- Hedgehog Signaling Pathway Studies
- Heat shock proteins research
- Colorectal Cancer Treatments and Studies
Uppsala University
2016-2025
University of Gothenburg
2010-2025
Science for Life Laboratory
2015-2024
Uppsala University Hospital
2010-2023
Deutschen Konsortium für Translationale Krebsforschung
2020
Stockholm University
2014
TU Dortmund University
2012-2014
Leibniz Research Centre for Working Environment and Human Factors
2012-2014
RWTH Aachen University
2012
KU Leuven
2012
Global gene expression profiling has been widely used in lung cancer research to identify clinically relevant molecular subtypes as well predict prognosis and therapy response. So far, the value of these multigene signatures clinical practice is unclear, biologic importance individual genes difficult assess, published virtually do not overlap.Here, we describe a novel single institute cohort, including 196 non-small cancers (NSCLC) with information long-term follow-up. Gene array data were...
Abstract Purpose: Although the central role of immune system for tumor prognosis is generally accepted, a single robust marker not yet available. Experimental Design: On basis receiver operating characteristic analyses, markers were identified from 60-gene B cell–derived metagene and analyzed in gene expression profiles 1,810 breast cancer; 1,056 non–small cell lung carcinoma (NSCLC); 513 colorectal; 426 ovarian cancer patients. Protein RNA levels examined paraffin-embedded tissue 330 The...
ObjectivesNon-small cell lung cancer (NSCLC) is a heterogeneous disease with unique combinations of somatic molecular alterations in individual patients, as well significant differences populations across the world regard to mutation spectra and frequencies. Here we aim describe mutational patterns linked clinical parameters population-based NSCLC cohort.Materials methodsUsing targeted resequencing status 82 genes was evaluated consecutive Swedish surgical cohort, consisting 352 patient...
Abstract The progression and metastatic capacity of solid tumors are strongly influenced by immune cells in the tumor microenvironment. In non–small cell lung cancer (NSCLC), accumulation anti-inflammatory tumor-associated macrophages (TAM) is associated with worse clinical outcome resistance to therapy. Here we investigated landscape NSCLC presence protumoral TAMs expressing macrophage receptor collagenous structure (MARCO). MARCO-expressing TAM numbers correlated increased occurrence...
The study of human macrophages and their ontogeny is an important unresolved issue. Here, we use a humanized mouse model expressing cytokines to dissect the development lung from hematopoiesis in vivo. Human CD34+ hematopoietic stem progenitor cells (HSPCs) generated three macrophage populations, occupying separate anatomical niches lung. Intravascular cell labeling, transplantation, fate-mapping studies established that classical CD14+ blood monocytes derived HSPCs migrated into tissue gave...
Background Aldosterone producing lesions are a common cause of hypertension, but genetic alterations for tumorigenesis have been unclear. Recently, either two recurrent somatic missense mutations (G151R or L168R) was found in the potassium channel KCNJ5 gene aldosterone adenomas. These alter selectivity filter and result Na+ conductance cell depolarization, stimulating production proliferation. Because similar mutation occurs Mendelian form primary aldosteronism, these appear to be...
Abstract Introduction Ductal carcinoma in situ (DCIS) is a non-invasive lesion of the breast that frequently detected by mammography and subsequently removed surgery. However, it estimated about half lesions would never have progressed into invasive cancer. Identifying DCIS cancer specific epigenetic understanding how these changes are involved triggering tumour progression important for better which at risk becoming invasive. Methods Quantitative DNA methylation analysis ABCB1, CDKN2A/p16...
Claudins (CLDNs) are central components of tight junctions that regulate epithelial‐cell barrier function and polarity. Altered CLDN expression patterns have been demonstrated in numerous cancer types lineage‐specific CLDNs proposed as therapy targets. The objective this study was to assess which fraction patients with non‐small‐cell lung (NSCLC) express CLDN6 CLDN18 isoform 2 (CLDN18.2). Protein CLDN18.2 examined by immunohistochemistry on a tissue microarray ( n = 355) transcript levels...
Cancer testis antigens (CTAs) are of clinical interest as biomarkers and present valuable targets for immunotherapy. To comprehensively characterize the CTA landscape non-small-cell lung cancer (NSCLC), we compared RNAseq data from 199 NSCLC tissues to normal transcriptome 142 samples 32 different organs. Of 232 CTAs currently annotated in Caner Testis Database (CTdatabase), 96 were confirmed NSCLC. obtain an unbiased profile NSCLC, applied stringent criteria on our set defined 90 genes...
Cancer immunity is based on the interaction of a multitude cells in spatial context tumour tissue. Clinically relevant immune signatures are therefore anticipated to fundamentally improve accuracy predicting disease progression.Through multiplex situ analysis we evaluated 15 cell classes 1481 samples. Single-cell and bulk RNAseq data sets were used for functional validation prognostic predictive associations.By combining information anti-tumoural CD8+ lymphocytes supportive CD68+CD163+...