A5030319622- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic and Hepatic Oncology Research
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Food composition and properties
- Medical Imaging and Pathology Studies
- Tissue Engineering and Regenerative Medicine
- Peptidase Inhibition and Analysis
- Cancer, Lipids, and Metabolism
- Wastewater Treatment and Reuse
- Bioinformatics and Genomic Networks
- Metabolism, Diabetes, and Cancer
- Microbial Metabolites in Food Biotechnology
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Innovations in Aquaponics and Hydroponics Systems
- Inflammatory Biomarkers in Disease Prognosis
- Cancer Genomics and Diagnostics
- Machine Learning in Bioinformatics
- Artificial Intelligence in Healthcare
Karolinska Institutet
2021-2025
Universitat de Barcelona
2014-2021
Inserm
2014-2021
Centre Hospitalier Universitaire de Grenoble
2014-2021
Institut polytechnique de Grenoble
2021
Centre National de la Recherche Scientifique
2021
Université Grenoble Alpes
2014-2021
Translational Innovation in Medicine and Complexity
2021
Laboratory of Fundamental and Applied Bioenergetics
2017
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2014
Cancer immunity is based on the interaction of a multitude cells in spatial context tumour tissue. Clinically relevant immune signatures are therefore anticipated to fundamentally improve accuracy predicting disease progression.Through multiplex situ analysis we evaluated 15 cell classes 1481 samples. Single-cell and bulk RNAseq data sets were used for functional validation prognostic predictive associations.By combining information anti-tumoural CD8+ lymphocytes supportive CD68+CD163+...
Cancer-associated fibroblasts (CAFs) are molecularly heterogeneous mesenchymal cells that interact with malignant and immune confer anti- protumorigenic functions. Prior in situ profiling studies of human CAFs have largely relied on scoring single markers, thus presenting a limited view their molecular complexity. Our objective was to study the complex spatial tumor microenvironment non-small cell lung cancer (NSCLC) multiple CAF biomarkers, identify novel subsets, explore associations...
The development of a reactive tumour stroma is hallmark progression and pronounced generally considered to be associated with clinical aggressiveness. variability between types regarding fraction, its prognosis associations, have not been systematically analysed.Using an objective machine-learning method we quantified the in 16 solid cancer from 2732 patients, representing retrospective tissue collections surgically resected primary tumours. Image analysis performed segmentation into stromal...
A perimetastatic capsule is a strong positive prognostic factor in liver metastases, but its origin remains unclear. Here, we systematically quantify the capsule's extent and cellular composition 263 patients with colorectal cancer metastases to investigate clinical significance origin. We show that survival improves proportionally increasing encapsulation decreasing tumor-hepatocyte contact. Immunostaining reveals gradual zonation of capsule, transitioning from benign-like NGFR
Studies have implied that fibroblasts may act as regulators of immune cells in the tumor microenvironment (TME). We investigated clinical relevance fibroblast activation protein (FAP) positive stroma high-grade serous ovarian cancer (HGSC) relation to CD8+ lymphocyte's infiltration. In a discovery cohort (N = 113) HGSC, expression FAP and CD8 TME was analyzed with immunohistochemistry. Results were correlated overall survival (OS) progression-free (PFS). The findings validated an independent...
While the clinical importance of CD8+ and CD3+ cells in colorectal cancer (CRC) is well established, impact other immune cell subsets less described. We sought to provide a detailed overview landscape CRC largest study date terms patient numbers situ analyzed types. Tissue microarrays from 536 patients were stained using multiplexed immunofluorescence panels, fifteen subclasses, representing adaptive innate immunity, analyzed. Overall, therapy-naïve clustered into an 'inflamed' 'desert'...
KRAS mutations in non-small cell lung cancer (NSCLC) are a predictor of resistance to EGFR-targeted therapies. Because approaches target RAS signaling have been unsuccessful, targeting metabolism might help develop new strategy that could overcome drug such cancer. In this study, we applied large screening quantitative proteomic analysis evidence key enzymes involved metabolic adaptations We carried out the two KRAS-mutated NSCLC lines (A549 and NCI-H460) non tumoral bronchial line (BEAS-2B)...
Growing evidence is showing that acetylation plays an essential role in cancer, but studies on the impact of KDAC inhibition (KDACi) metabolic profile are still their infancy. Here, we analyzed, by using iTRAQ-based quantitative proteomics approach, changes proteome KRAS-mutated non-small cell lung cancer (NSCLC) A549 cells response to trichostatin-A (TSA) and nicotinamide (NAM) under normoxia hypoxia. Part this was further validated molecular biochemical analyses correlated with...
Nuclear factor erythroid 2-related 2 (NRF2) protein expression promotes cancer progression in non-small cell lung (NSCLC). However, its role the clinical setting has not been established. We retrospectively analyzed data from 304 patients with surgically removed NSCLC. Multiplex antibody staining of NRF2 and thioredoxin reductase 1 (TrxR1) was conducted scored cytokeratin-positive (CK+) cells within whole-tissue core as well tumor stromal compartments each tissue microarray (TMA) core. A...
Background: Cancer immunity is based on the interaction of a multitude cells in spatial context tumor tissue. Immune cell patterns are connected to patient prognosis and response therapy. Clinically relevant immune signatures therefore anticipated fundamentally improve accuracy predicting disease progression.Methods Findings: Through multiplex situ analysis pipeline we evaluated 15 classes 352 colorectal cancers associated infiltration with clinical outcome. By combining prognostic...
Abstract Immune cells in the microenvironment shape tumor development and progression. The prognostic value of T-cell-based immune scores exceeds those clinical parameters colon cancer, but reflects only a part anti-tumor response. Here, we assessed 15 distinct cell classes identified simple signature based on pro- anti-tumoral microenvironment. ratio cytotoxic lymphocytes to supportive macrophages predicted survival better than state-of-art score cancer had highest relative contribution...
Summary Immune cells in the microenvironment shape tumor development and progression. Through situ analyses we assessed 15 immune cell classes 352 colorectal cancers identified a simple prognostic signature based on ratio of anti-tumoral CD8+ lymphocytes to tumor-supportive M2-like macrophages (CD68+CD163+) microenvironment. The ability this was superior state-of-art score also demonstrated four other types. Single-cell these as source complement C1q, CD8A C1QA gene expression levels bulk...