A5030572866- Testicular diseases and treatments
- Cancer Immunotherapy and Biomarkers
- Sperm and Testicular Function
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Urologic and reproductive health conditions
- Lung Cancer Research Studies
- Sarcoma Diagnosis and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Peptidase Inhibition and Analysis
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
- Prostate Cancer Treatment and Research
- Epilepsy research and treatment
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Brain Metastases and Treatment
- Pharmacological Effects and Toxicity Studies
- Drug-Induced Hepatotoxicity and Protection
- Radiomics and Machine Learning in Medical Imaging
- Reproductive Biology and Fertility
- Immune cells in cancer
- Pancreatic function and diabetes
Karolinska Institutet
2019-2022
University of the Basque Country
2011-2015
Cells Therapy (Poland)
2013
University of Miami
2005
Universidad de Málaga
2000
Fibroblasts regulate tumor growth and immune surveillance. Here, we study FAP, PDGFβR α-SMA fibroblast markers in a well-annotated clinical cohort of non-small-cell lung cancer (NSCLC) for analyses associations with cell infiltration, mutation status survival.A NSCLC was subjected to IHC stromal expression CD8 density. Fibroblast markers-related measurements were analyzed regard potential density, genetic driver mutations, survival PD-L1 the whole subsets patients.High FAP identified as an...
Cancer-associated fibroblasts (CAFs) are molecularly heterogeneous mesenchymal cells that interact with malignant and immune confer anti- protumorigenic functions. Prior in situ profiling studies of human CAFs have largely relied on scoring single markers, thus presenting a limited view their molecular complexity. Our objective was to study the complex spatial tumor microenvironment non-small cell lung cancer (NSCLC) multiple CAF biomarkers, identify novel subsets, explore associations...
We aimed to assess if the discrepant prognostic information between Programmed Death Ligand 1 (PD-L1) protein versus mRNA expression in early breast cancer (BC) could be attributed heterogeneity its expression. PD-L1 and BC tissue microarrays from two clinical patient cohorts were evaluated (105 patients; cohort 1: untreated; 2: neoadjuvant chemotherapy-treated). Immunohistochemistry (IHC) with SP142, SP263 was performed. using bulk gene RNA-FISH RNAscope®, latter scored a semi-quantitative...
Abstract Emerging data indicate that genomic alterations can shape immune cell composition in early breast cancer. However, there is a need for complementary imaging and sequencing methods the quantitative assessment of combined somatic copy number alteration (SCNA) profiling pathological samples. Here, we tested feasibility three approaches—CUTseq, high-throughput low-input SCNA profiling, multiplexed fluorescent immunohistochemistry (mfIHC) digital-image analysis (DIA) immuno-profiling-...
Summary Today cancer research studies have highlighted the role of cancer–stroma interaction in regulation invasive processes. However, very little is known about cell‐to‐cell relationships between germinal cells and somatic ones belong to their close environment, particularly at early invasion stages. Here, we studied potential seminiferous peritubular myoid ( PTC s), as part reactive stroma, like tumor myofibroblast, progression embryonal carcinoma EC ). To this end, show results on vitro...
Nuclear factor erythroid 2-related 2 (NRF2) protein expression promotes cancer progression in non-small cell lung (NSCLC). However, its role the clinical setting has not been established. We retrospectively analyzed data from 304 patients with surgically removed NSCLC. Multiplex antibody staining of NRF2 and thioredoxin reductase 1 (TrxR1) was conducted scored cytokeratin-positive (CK+) cells within whole-tissue core as well tumor stromal compartments each tissue microarray (TMA) core. A...
Abstract Background: Given the emerging role of immune-related biomarkers in breast cancer (BC), little is known about specific immune cell composition patterns and genomic alterations how these could interact early BC setting. The aim this proof-of-principle study to describe immuno-genomic correlates within tumor microenvironment using newly developed techniques for multiplexed fluorescent immunohistochemistry (mfIHC, Mezheyeuski et al., J Pathol. 2018) high-throughput low-input genome...