A5001915657- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Immune cells in cancer
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Immune Cell Function and Interaction
- Radiomics and Machine Learning in Medical Imaging
- vaccines and immunoinformatics approaches
- Lung Cancer Research Studies
- Lung Cancer Diagnosis and Treatment
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Fibroblast Growth Factor Research
- Cancer survivorship and care
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Transplantation: Methods and Outcomes
- Lymphoma Diagnosis and Treatment
- COVID-19 diagnosis using AI
- Bioinformatics and Genomic Networks
- Genomics, phytochemicals, and oxidative stress
- Plant-derived Lignans Synthesis and Bioactivity
Uppsala University
2017-2025
Science for Life Laboratory
2021
Åbo Akademi University
2007
Red Cross University College of Nursing
2007
Saint Göran Hospital
2004
Abstract Semiquantitative assessment of immune markers by immunohistochemistry (IHC) has significant limitations for describing the diversity response in cancer. Therefore, we evaluated a fluorescence‐based multiplexed immunohistochemical method combination with multispectral imaging system to quantify infiltrates situ environment non‐small‐cell lung cancer (NSCLC). A tissue microarray including 57 NSCLC cases was stained antibodies against CD8, CD20, CD4, FOXP3, CD45RO, and pan‐cytokeratin,...
The fragmentation pattern of 30 compounds belonging to different classes the lignan family was studied by liquid chromatography-electrospray ionization ion-trap mass spectrometry. On basis observed patterns, identification types lignans achieved. For example, dibenzylbutyrolactone showed a characteristic pathway loss 44 Da (CO(2)) from lactone moiety, whereas dibenzylbutanediols 48 combined formaldehyde and water 1,4-butanediol moiety. Lignan glycosides readily lost sugar residue give parent...
Cancer immunity is based on the interaction of a multitude cells in spatial context tumour tissue. Clinically relevant immune signatures are therefore anticipated to fundamentally improve accuracy predicting disease progression.Through multiplex situ analysis we evaluated 15 cell classes 1481 samples. Single-cell and bulk RNAseq data sets were used for functional validation prognostic predictive associations.By combining information anti-tumoural CD8+ lymphocytes supportive CD68+CD163+...
IntroductionImmune cells in the tumour microenvironment are associated with prognosis and response to therapy. We aimed comprehensively characterise spatial immune phenotypes mutational clinicopathological background of non–small cell lung cancer (NSCLC).MethodsWe established a multiplexed fluorescence imaging pipeline spatially quantify 13 subsets 359 NSCLC cases: CD4 effector (CD4-Eff), regulatory (CD4-Treg), CD8 (CD8-Eff), (CD8-Treg), B-cells, natural killer cells, T-cells, M1 macrophages...
Fibroblasts regulate tumor growth and immune surveillance. Here, we study FAP, PDGFβR α-SMA fibroblast markers in a well-annotated clinical cohort of non-small-cell lung cancer (NSCLC) for analyses associations with cell infiltration, mutation status survival.A NSCLC was subjected to IHC stromal expression CD8 density. Fibroblast markers-related measurements were analyzed regard potential density, genetic driver mutations, survival PD-L1 the whole subsets patients.High FAP identified as an...
Cancer-associated fibroblasts (CAFs) are molecularly heterogeneous mesenchymal cells that interact with malignant and immune confer anti- protumorigenic functions. Prior in situ profiling studies of human CAFs have largely relied on scoring single markers, thus presenting a limited view their molecular complexity. Our objective was to study the complex spatial tumor microenvironment non-small cell lung cancer (NSCLC) multiple CAF biomarkers, identify novel subsets, explore associations...
The development of a reactive tumour stroma is hallmark progression and pronounced generally considered to be associated with clinical aggressiveness. variability between types regarding fraction, its prognosis associations, have not been systematically analysed.Using an objective machine-learning method we quantified the in 16 solid cancer from 2732 patients, representing retrospective tissue collections surgically resected primary tumours. Image analysis performed segmentation into stromal...
Amplification of fibroblast growth factor receptor 1 (FGFR1) in non-small cell lung cancer (NSCLC) has been considered as an actionable drug target. However, pan-FGFR tyrosine kinase inhibitors did not demonstrate convincing clinical efficacy FGFR1-amplified NSCLC patients. This study aimed to characterise the molecular context FGFR1 expression and define biomarkers predictive inhibitor response. In this study, 635 samples were characterised for protein by immunohistochemistry copy number...
While the clinical importance of CD8+ and CD3+ cells in colorectal cancer (CRC) is well established, impact other immune cell subsets less described. We sought to provide a detailed overview landscape CRC largest study date terms patient numbers situ analyzed types. Tissue microarrays from 536 patients were stained using multiplexed immunofluorescence panels, fifteen subclasses, representing adaptive innate immunity, analyzed. Overall, therapy-naïve clustered into an 'inflamed' 'desert'...
T-cell activation and clonal expansion are essential for the efficacy of immunotherapy in non-small cell lung cancer (NSCLC) patients. Since distribution clones might provide insights into immunogenic mechanisms, we determined α/β TCR clonality using RNA-sequencing from frozen tumor tissue 182 NSCLC patients paired results with extensive situ image sequence analyses immune microenvironment NSCLC. (Gini index) patterns ranged high clone diversity evenness index low) to dominance low high)....
Abstract Introduction Tertiary lymphoid structures (TLS) are lymphocyte aggregates resembling secondary organs and pivotal in cancer immunity. The ambiguous morphological definition of TLS makes it challenging to ascertain their clinical impact on patient survival response immunotherapy. Objectives This study aimed characterize hematoxylin-eosin tissue sections from lung patients, assessing occurrence relation the local immune environment, mutational background, outcome. Methods Two...
Abstract Immune cells of the tumor microenvironment are central but erratic targets for immunotherapy. The aim this study was to characterize novel patterns immune cell infiltration in non‐small lung cancer (NSCLC) relation its molecular and clinicopathologic characteristics. Lymphocytes (CD3+, CD4+, CD8+, CD20+, FOXP3+, CD45RO+), macrophages (CD163+), plasma (CD138+), NK (NKp46+), PD1+, PD‐L1+ were annotated on a tissue microarray including 357 NSCLC cases. Somatic mutations analyzed by...
The antigenic repertoire of tumors is critical for successful anti-cancer immune response and the efficacy immunotherapy. Cancer-testis antigens (CTAs) are targets humoral cellular reactions. We aimed to characterize CTA expression in non-small cell lung cancer (NSCLC) context microenvironment. Of 90 CTAs validated by RNA sequencing, eight (DPEP3, EZHIP, MAGEA4, MAGEB2, MAGEC2, PAGE1, PRAME, TKTL1) were selected immunohistochemical profiling tissues from 328 NSCLC patients. was compared with...
Gene amplification is considered to be one responsible cause for upregulation of Programmed Death Ligand-1 (PD-L1) in non-small cell lung cancer (NSCLC) and represent a specific molecular subgroup possibly associated with immunotherapy response. Our aim was analyze the frequency PD-L1 amplification, its relation mRNA protein expression, characterize immune microenvironment amplified cases. The study based on two independent NSCLC cohorts, including 354 349 cases, respectively. Tissue...
Earlier findings in different care settings have revealed that women with breast cancer admitted to anthroposophical clinics (complementary care) initially had lower quality of life scores compared those conventional care, but the after 1 year increased significantly. The hospital this study offers integrated and healthcare therapies. present examines experiences among during 1-year follow-up original study. A second aim was seek profiles differences between 37 matched pairs cancer. mean age...
AbstractBackground: The main purpose of this study was to compare omeprazole (ome) plus two antibiotics (OMC) with placebo (OP) regard gastric ulcer relapse for a period 2 years in patients who were Helicobacter pylori-positive at inclusion. Methods: Using double-blind randomization 125 treated either OMC (ome 20 mg b.i.d., metronidazole 400 clarithromycin 250 b.i.d.) (n = 64) or OP and placebo) 61) 1 week, followed by ome 20-40 o.d. until healing confirmed endoscopically after 4, 8 12...
We developed a deep learning framework that helps to automatically identify and segment lung cancer areas in patients' tissue specimens. The study was based on cohort of patients operated at the Uppsala University Hospital. tissues were reviewed by pathologists then cores compiled micro-arrays (TMAs). For experiments, hematoxylin-eosin stained slides from 712 scanned manually annotated. Then these scans annotations used train segmentation models framework. performance evaluated fully...
Immunohistochemistry (IHC) is the accepted standard for spatial analysis of protein expression in tissues. IHC widely used cancer diagnostics and basic research. The development new antibodies to proteins with unknown patterns has created a demand thorough validation. We have applied resources from Human Protein Atlas project Antibody Portal at National Cancer Institute generate data 12 across 39 cell lines 37 normal human tissue types. outcome on consecutive sections both microarrays using...
The immune microenvironment is involved in fundamental aspects of tumorigenesis, and scores are now being developed for clinical diagnostics.
Abstract The splice variants specific protein expression in tumor cells presents promising targets for cancer therapy. concept is supported by the recent introduction of variant-specific antibody against FGFR2 IIIb (FGFR2b) gastric cancer. Indeed, signaling two FGFR2b and IIIc (FGFR2c), whose mutually exclusive on a cellular level, mediated different ligands distinct downstream responses that can contribute to many aspects tumorigenesis. Our study aimed characterize 2c non-malignant lung...