A5059332727- Occupational and environmental lung diseases
- Medical Imaging and Pathology Studies
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Inflammatory mediators and NSAID effects
- Cancer Cells and Metastasis
- Renal and related cancers
- Pleural and Pulmonary Diseases
- Immune cells in cancer
- Tissue Engineering and Regenerative Medicine
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer-related Molecular Pathways
- Cancer Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Testicular diseases and treatments
- Ferroptosis and cancer prognosis
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Click Chemistry and Applications
- Neuroblastoma Research and Treatments
- Cell Adhesion Molecules Research
- Lung Cancer Diagnosis and Treatment
- Colorectal and Anal Carcinomas
Uppsala University
2011-2025
Uppsala University Hospital
2021
Gävle Hospital
2009
Karolinska Institutet
1999-2005
Karolinska University Hospital
2003-2005
National Institute of Oncology
1999-2000
BM Központi Kórház
1995
IntroductionImmune cells in the tumour microenvironment are associated with prognosis and response to therapy. We aimed comprehensively characterise spatial immune phenotypes mutational clinicopathological background of non–small cell lung cancer (NSCLC).MethodsWe established a multiplexed fluorescence imaging pipeline spatially quantify 13 subsets 359 NSCLC cases: CD4 effector (CD4-Eff), regulatory (CD4-Treg), CD8 (CD8-Eff), (CD8-Treg), B-cells, natural killer cells, T-cells, M1 macrophages...
Abstract Introduction Tertiary lymphoid structures (TLS) are lymphocyte aggregates resembling secondary organs and pivotal in cancer immunity. The ambiguous morphological definition of TLS makes it challenging to ascertain their clinical impact on patient survival response immunotherapy. Objectives This study aimed characterize hematoxylin-eosin tissue sections from lung patients, assessing occurrence relation the local immune environment, mutational background, outcome. Methods Two...
Abstract Malignant mesothelioma is a tumour frequently accompanied by an effusion with elevated hyaluronan levels. To distinguish malignant mesothelioma, adenocarcinoma, and reactive benign mesothelium cytological histological methods including immunocytochemistry major diagnostic challenge. The Wilms' susceptibility gene 1 (WT1), expressed during transition of mesenchyme to epithelial tissues, regarded as marker for the mesothelial lineage. Its effect on cell phenotype may be regulated via...
Abstract Immune cells of the tumor microenvironment are central but erratic targets for immunotherapy. The aim this study was to characterize novel patterns immune cell infiltration in non‐small lung cancer (NSCLC) relation its molecular and clinicopathologic characteristics. Lymphocytes (CD3+, CD4+, CD8+, CD20+, FOXP3+, CD45RO+), macrophages (CD163+), plasma (CD138+), NK (NKp46+), PD1+, PD‐L1+ were annotated on a tissue microarray including 357 NSCLC cases. Somatic mutations analyzed by...
An immunocytochemical battery comprising 9 antibodies specifically distinguishes 80% of the epithelial malignant mesotheliomas from adenocarcinomas.The discriminatory power to calretinin was tested together with this determine whether performance thereby could be improved.The study comprises 119 or mixed phenotype and 57 adenocarcinoma metastases in pleural cavity.The differences between 2 groups were highly significant for all recorded parameters, but typical reactivity parameters seen only...
Aim: Inhibition of cyclooxygenase-2 (COX-2) is proposed as a treatment option in several cancer types. However, non-small cell lung (NSCLC), phase III trials have failed to demonstrate benefit adding COX-2 inhibitors standard chemotherapy. The aim this study was analyze expression tumor and stromal cells predictive biomarker for inhibition.Methods: In multicenter trial, 316 patients with advanced NSCLC were randomized receive celecoxib (400 mg b.i.d.) or placebo up one year addition two-drug...
Human mesothelial cells obtained from benign effusions retain their proliferative capacity and grow uniformly either with a fibroblastic or epithelioid morphology in vitro. These cultures therefore provide model for the process of differentiation vivo. To study this differentiation, we isolated differentially expressed genes by suppression subtractive hybridization. Of nine found to be overexpressed cells, three are matrix-associated (integrin alpha5, collagen binding protein 2, human...
Aim-To determine whether assays of carcinoembryonic antigen (CEA) and cholesterol in ascites add diagnostic value to cytology.Methods-The additional eYcacy the biochemical was studied ascitic fluid from 130 patients, whom 57 had peritoneal carcinomatosis.All diagnoses were verified by subsequent necropsy and/or histology.Results-CEA concentrations over 5 ng/ ml indicated carcinomas, occasionally without involvement tumour.However, increased values significantly more common cancer with (p <...
Background: Inflammatory bowel disease (IBD; mainly ulcerative colitis and Crohn’s disease) is associated with the development of colorectal cancer (CRC) referred to as colitis-associated (CAC). In inflammatory flares IBD, production luminal nitric oxide (NO) increases due increased inducible synthase (iNOS) activity in inflamed tissue. It believed that iNOS parallels pro-inflammatory interleukin-1β (IL-1β). How these biomarkers relate CAC pathogenesis or survival unknown.
 Aim: The...
Xenografting is widely used for assessing in vivo pluripotency of human stem cell populations. Here, we report on early to late events the development mature experimental teratoma from a well-characterized embryonic (HESC) line, HS181. The results show an process, increasingly chaotic. Active proliferation derived cellular progeny was detected already at day 5, and characterized by appearance multiple sites engraftment, with structures single or pseudostratified columnar epithelium...
Accurate and reliable diagnosis is essential for lung cancer treatment. The study aim was to investigate interpathologist diagnostic concordance pulmonary tumours according WHO criteria.Fifty-two unselected bronchial biopsies were diagnosed by a thoracic pathologist based on broad spectrum of immunohistochemical (IHC) stainings, molecular data clinical/radiological information. Slides stained with H&E, thyroid transcription factor-1 (TTF-1) clone SPT24 p40 scanned provided digitally 20...
PPM1D is a negative regulator of p53 and genomic aberrations resulting in increased activity have been observed cancers different origins, indicating that has oncogenic properties. We established transgenic mouse model overexpressing showed these mice developed wide variety cancers. PPM1D-expressing tumors phenotypically genetically similar to with dysfunctional p53. T-cell lymphoblastic lymphoma was the most frequent cancer (55%) followed by adenocarcinomas (24%), leukemia (12%) other solid...
Background. Atelectasis occurs after a well performed endotracheal suction. Clinical studies have shown that recruitment manoeuvres added suction during mechanical ventilation restore lung function. Repetitive over-distension is, however, harmful for the lung, and effects of adding larger breath, directly repeated were therefore investigated. Methods. Twelve healthy anaesthetized pigs randomized into two groups: one without with breath manoeuvre (RBM), i.e. 15 cmH2O above inspiratory...
For clinically relevant studies on melanoma progression and invasiveness, in vivo experimental systems with a human cellular microenvironment would be advantageous. We have compared tumor formation from cutaneous malignant cell line (BL), after injection as conventional xenografts the mouse, or when injected into predominantly species-specific environment of embryonic stem cell-derived teratoma induced mouse (the hEST model). The resulting histology was generally analogous, both showing...
Patients with non-small cell lung cancer (NSCLC) harboring a ROS proto-oncogene 1 (ROS1)-rearrangement respond to treatment ROS1 inhibitors. To distinguish these rare cases, screening immunohistochemistry (IHC) for protein expression has been suggested. However, the reliability of such an assay and comparability antibody clones debated. Therefore we evaluated diagnostic performance current detection strategies ROS1-rearrangement in two NSCLC-patient cohorts.Resected tissue samples,...