A5002090343- Cancer Immunotherapy and Biomarkers
- Breast Cancer Treatment Studies
- Atherosclerosis and Cardiovascular Diseases
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Antioxidant Activity and Oxidative Stress
- Cardiac Ischemia and Reperfusion
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- Breast Lesions and Carcinomas
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Research Studies
- Nitric Oxide and Endothelin Effects
- Medical Imaging Techniques and Applications
- Inflammatory Bowel Disease
- Eicosanoids and Hypertension Pharmacology
- Diagnosis and Treatment of Venous Diseases
- Radiomics and Machine Learning in Medical Imaging
- Molecular Biology Techniques and Applications
University Hospital of Lausanne
2005-2022
University of Lausanne
2006-2022
Medizin Campus Bodensee
2013-2020
Heidelberg University
1989-2015
Johannes Gutenberg University Mainz
1999-2015
University of Washington
1993-2015
Hoshi University
2015
SIB Swiss Institute of Bioinformatics
2013
Ludwig Cancer Research
2013
Cancer Research Center
2013
Signal transducer and activator of transcription (STAT) 3 is a pleiotropic factor with important functions in cytokine signaling variety tissues. However, the role STAT3 intestinal epithelium not well understood. We demonstrate that development colonic inflammation associated induction activity epithelial cells (IECs). Studies genetically engineered mice showed activation dextran sodium sulfate colitis dependent on interleukin (IL)-22 rather than IL-6. IL-22 was secreted by CD11c+ response...
Abstract Estrogen receptor (ER) expression and proliferative activity are established prognostic factors in breast cancer. In a search for additional motifs, we analyzed the gene patterns of 200 tumors patients who were not treated by systemic therapy after surgery using discovery approach. After performing hierarchical cluster analysis, identified coregulated genes related to biological process proliferation, steroid hormone expression, as well B-cell T-cell infiltration. We calculated...
The overexpression of glucose transporters, especially Glut-1, is a common characteristic human malignancies, including head and neck carcinoma. Recently, the assessment metabolism in tumor with [(18)F]-2-fluoro-2 deoxy-D-glucose (FDG) positron emission tomography (FDG-PET) has been used to identify particularly aggressive tumors. authors tested hypothesis that both transport its play key role progression oral squamous cell carcinoma (OSCC).Retrospective analysis Glut-1 expression was...
Abstract Purpose: Although the central role of immune system for tumor prognosis is generally accepted, a single robust marker not yet available. Experimental Design: On basis receiver operating characteristic analyses, markers were identified from 60-gene B cell–derived metagene and analyzed in gene expression profiles 1,810 breast cancer; 1,056 non–small cell lung carcinoma (NSCLC); 513 colorectal; 426 ovarian cancer patients. Protein RNA levels examined paraffin-embedded tissue 330 The...
The benefit of quantifying estrogen receptor (ER) and progesterone (PR) expression in breast cancer is well established. However, routine diagnosis, often quantified arbitrary scores with high inter- intraobserver variability. In this study we tested the validity an image analysis system employing inexpensive, commercially available computer software on a personal computer. series 28 invasive ductal cancers, immunohistochemical determinations ER PR were performed, along biochemical analyses...
Hypertension is a side effect of systemically administered glucocorticoids, but the underlying molecular mechanism remains poorly understood. Ingestion dexamethasone by rats telemetrically instrumented increased blood pressure progressively over 7 days. Plasma concentrations Na + and K urinary excretion remained constant, excluding mineralocorticoid-mediated mechanism. NO 2 − /NO 3 (the oxidation products NO) decreased to 40%, expression endothelial synthase (NOS III) was found...
Simultaneous detection of two different antigens on paraffin-embedded and frozen tissues can be accomplished by double immunohistochemistry. However, many chromogen systems suffer from signal overlap, precluding definite quantification. To separate quantitatively analyze the chromogens, we imported images into a Macintosh computer using CCD camera attached to diagnostic microscope used Photoshop software for recognition, selection, separation colors. We show here that Photoshop-based image...
Abstract Although IL-12 and IL-23 share the common p40 subunit, IL-23, rather than IL-12, seems to drive pathogenesis of experimental autoimmune encephalomyelitis arthritis, because IL-23/p19 knockout mice are protected from disease. In contrast, we describe in this study that newly created LacZ knockin deficient for p19 were highly susceptible development T cell-mediated TNBS colitis showed even more severe wild-type by endoscopic histologic criteria. Subsequent studies revealed dendritic...
Leukotriene (LT)B4 promotes leukocyte chemotaxis and adhesion to the endothelium of postcapillary venules. The cysteinyl leukotrienes, LTC4, LTD4, LTE4, elicit macromolecular leakage from this vessel segment. Both venules hallmark microcirculatory failure after ischemia-reperfusion, suggesting a role leukotrienes as mediators ischemia-reperfusion injury. Using dorsal skinfold chamber model for intravital fluorescence microscopy microcirculation in striated muscle awake hamsters sequential...
Background — On the basis of our concept that atherosclerosis has an immunopathological background, we tested whether activation innate immune system influences its progression. Methods and Results Hypercholesterolemic (0.5% wt/wt diet) rabbits received either repeated intravenous injections endotoxin ( Escherichia coli lipopolysaccharide 1.25 to 2.5 μg, once per week) or a self-limiting cutaneous Staphylococcus aureus infection with without quinolone antibiotic. Measured laboratory...
BACKGROUND Despite the clinical efficacy of endovenous laser treatment (EVLT), its mode action is incompletely understood. OBJECTIVE To evaluate role intravascular blood for effective transfer thermal damage to vein wall through absorption energy. METHODS Laser energy (15 J/pulse, 940 nm) was endovenously administered explanted greater saphenous (GSV) segments filled with (n = 5) or normal saline in addition GSVs under vivo conditions immediately prior stripping. Histopathology performed on...
BackgroundGut bacteria trigger colitis in animal models and are suspected to aggravate inflammatory bowel diseases. We have recently reported that Escherichia coli accumulates murine ileitis exacerbates small intestinal inflammation via Toll-like receptor (TLR) signaling.Methodology Principal FindingsBecause knowledge on shifts the microflora during is limited, we performed a global survey of colon flora C57BL/10 wild-type (wt), TLR2-/-, TLR4-/-, TLR2/4-/- mice treated for seven days with...
Abstract Dendritic cells (DC) manipulated ex vivo can induce tumor immunity in experimental murine models. To improve DC-based vaccination, we studied whether DC maturation affects the T cell-activating potential vitro and induction of vivo. Maturation bone marrow-derived was induced by GM-CSF plus IL-4 alone or further addition TNF-α a cytidine-phosphate-guanosine (CpG)-containing oligonucleotide (ODN-1826), which mimics immunostimulatory effect bacterial DNA. Flow cytometric analysis...
Background— We have recently demonstrated that activity of red blood cell glutathione peroxidase-1 is inversely associated with the risk cardiovascular events in patients coronary artery disease. The present study analyzed effect deficiency on atherogenesis apolipoprotein E-deficient mouse. Methods and Results— Female mice without were placed a Western-type diet for another 6, 12, or 24 weeks. After weeks diet, double-knockout (GPx-1 −/− ApoE ) developed significantly more atherosclerosis...
<b><i>Background:</i></b> Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation due to dysregulation of the mucosal immune system. The cytokines IL-1β and IL-18 appear early in their pro-forms are processed via caspase-1-activating multiprotein complex, Nlrp3 inflammasome. Previously, we reported that uptake dextran sodium sulfate (DSS) macrophages activates inflammasome Nlrp3<sup>–/–</sup> mice protected acute DSS colitis...