A5076952708- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Metabolomics and Mass Spectrometry Studies
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Analytical Chemistry and Chromatography
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- Legume Nitrogen Fixing Symbiosis
- Cardiac Ischemia and Reperfusion
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Retinal Development and Disorders
- Advanced Glycation End Products research
- Cancer-related molecular mechanisms research
Martin Luther University Halle-Wittenberg
2016-2025
Luther University
2011-2024
Merck (Germany)
2013-2023
Ono Pharmaceutical (United States)
2022
Wittenberg University
2018
University Medical Center Freiburg
1994-2013
Praxis für Humangenetik
2008-2012
University of Cologne
2010
University of Freiburg
1997-2009
University of Amsterdam
2009
Chemical crosslinking in combination with mass spectrometry has matured into an alternative approach to derive low-resolution structural information of proteins and protein complexes. Yet, one the major drawbacks this strategy remains lack software that is able handle large MS datasets are created after chemical enzymatic digestion reaction mixtures. Here, we describe a software, termed StavroX, which been specifically designed for analyzing highly complex datasets. The StavroX was evaluated...
CID-MS/MS cleavable cross-linkers hold an enormous potential for automated analysis of cross-linked products, which is essential conducting structural proteomics studies. The created characteristic fragment ion patterns can easily be used assignment and discrimination products. To date, there are only a few software solutions available that make use these properties, but none allows MeroX fills this gap presents powerful tool protein 3D-structure in combination with MS/MS cross-linkers. We...
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by SARS-CoV-2 virus. We developed a simple, MS-based method specifically detect proteins from gargle solution samples patients. The protocol consists an acetone precipitation tryptic digestion contained within solution, followed targeted MS analysis. Our methodology identifies unique peptides originating nucleoprotein....
To compare the diagnostic accuracy of magnetic resonance (MR) imaging with that positron emission tomography (PET) 2-[fluorine 18]fluoro-2-deoxy-D-glucose (FDG) for detecting metastatic lymph nodes in patients cervical cancer.Before radical hysterectomy and pelvic lymphadenectomy 35 International Federation Gynecology Obstetrics stage IB or II cancer, abdominal FDG-PET MR were performed. Malignancy criteria a node diameter 1 cm more at focally increased FDG uptake PET. The findings compared...
Background The pathomorphological substrate of complicated coronary atherosclerotic lesions underlying unstable angina is characterized by a localized chronic inflammatory process. Functionally, associated with demonstrate an enhanced vasoreactivity. Endothelin-1 potent vasoconstrictor peptide produced not only endothelial cells but also macrophages and polymorphonuclear leukocytes, the cell types characteristic inflammation. Methods Results By use immunohistochemical techniques, we examined...
Background — Atherosclerosis is a chronic inflammatory response of the arterial wall to injury. Macrophage migration inhibitory factor (MIF), cytokine with potent functions, was thus considered be important in atherosclerotic lesion evolution. Methods and Results We studied presence distribution MIF immunoreactivity (MIF-IR) mRNA internal mammary arteries normal histology plaques different stages human atherosclerosis. To address potential role for coactivator Jab1 as cellular mediator...
Chemical cross-linking combined with a subsequent enzymatic cleavage of the created cross-linked complex and mass spectrometric analysis resulting peptide mixture presents an alternative approach to high-resolution analysis, such as NMR spectroscopy or X-ray crystallography, obtain low-resolution protein structures gain insight into interfaces. Here, we describe novel urea-based cross-linker, which allows distinguishing different products by collision-induced dissociation (CID) tandem MS...
Background — On the basis of our concept that atherosclerosis has an immunopathological background, we tested whether activation innate immune system influences its progression. Methods and Results Hypercholesterolemic (0.5% wt/wt diet) rabbits received either repeated intravenous injections endotoxin ( Escherichia coli lipopolysaccharide 1.25 to 2.5 μg, once per week) or a self-limiting cutaneous Staphylococcus aureus infection with without quinolone antibiotic. Measured laboratory...
The CCR4-NOT complex is the main enzyme catalyzing deadenylation of mRNA. We have investigated composition this in Drosophila melanogaster by immunoprecipitation with a monoclonal antibody directed against NOT1. CCR4, CAF1 (=POP2), NOT1, NOT2, NOT3, and CAF40 subunits were associated stable complex, but NOT4 was not. Factors known to be involved mRNA regulation prominent among other proteins coprecipitated as analyzed mass spectrometry. localized mostly cytoplasm did not appear major...
We present a cross-linking/mass spectrometry workflow for performing proteome-wide cross-linking analyses within 1 week. The is based on the commercially available mass spectrometry-cleavable cross-linker disuccinimidyl dibutyric urea and can be employed by every lab having access to spectrometer with tandem capabilities. provide an updated version 2.0 of freeware software tool MeroX, at www.StavroX.com, that allows us conduct fully automated reliable studies delivering insights into...
BACKGROUND. There is increasing evidence for the role of impaired intestinal permeability in obesity and associated metabolic diseases. Zonulin an established serum marker identical to pre-haptoglobin2. Here, we aimed investigate relationship between circulating zonulin traits related obesity. METHODS. Serum was measured by using a widely used commercial ELISA kit 376 subjects from metabolically well-characterized cohort Sorbs Germany. In addition, haptoglobin genotype determined DNA samples...
Nitrogen fixation is an agronomically and environmentally important process catalyzed by bacterial nitrogenase within legume root nodules. These unique symbiotic organs have high metabolic rates produce large amounts of reactive oxygen species that may modify proteins irreversibly. Here, we examined two types oxidative posttranslational modifications nodule proteins: carbonylation, which occurs direct oxidation certain amino acids or interaction with aldehydes arising from cell membrane...
A major challenge in cross-linking/mass spectrometry (MS) is targeting carboxyl functions proteins under physiological conditions that do not disturb the protein's conformation. Cross-linking of glutamic acid and aspartic residues will greatly expand scope structural mass spectrometry. We discovered carboxyl-reactive cross-linkers have already been employed for many years cross-linking/MS studies, yet a completely different context. Diazirine-based cross-linkers, such as photomethionine...
Abstract α‐Synuclein (α‐syn) is an intrinsically disordered protein (IDP) that undergoes liquid‐liquid phase separation (LLPS), fibrillation, and forms insoluble intracellular Lewy bodies in neurons, which are the hallmark of Parkinson's Disease (PD). Neurotoxicity precedes formation aggregates might be related to α‐syn LLPS. The molecular mechanisms underlying early stages LLPS still elusive. To obtain structural insights into upon LLPS, we take advantage cross‐linking/mass spectrometry...
The 3' ends of almost all eukaryotic mRNAs are generated in an essential two-step processing reaction: endonucleolytic cleavage extended precursor followed by the addition a poly(A) tail. By reconstituting reaction from overproduced and purified proteins, we provide minimal list 14 polypeptides that two stimulatory for RNA processing. In depending on polyadenylation signal AAUAAA, reconstituted system cleaves pre-mRNA at single preferred site corresponding to one used vivo. Among factor I...
Congestive heart failure is a leading cause of mortality in developed countries. Myocardial hypertrophy resulting from hypertension often precedes failure. Understanding the signaling underlying cardiac and major interest. Here, we identified Fas receptor activation, classical death signal causing apoptosis via activation caspase cascade many cell types, as novel pathway mediating cardiomyocyte vitro vivo. by ligand induced hypertrophic response cultured cardiomyocytes, which was dependent...
Heart failure as a consequence of sustained hemodynamic overload is among the most prevalent diseases in developed countries. The aim present study was to investigate specific role endothelial nitric oxide synthase (eNOS) pressure overload-induced left ventricular (LV) hypertrophy.Chronic pressure-overload LV hypertrophy induced by abdominal aortic banding (AC) wild-type (WT) and eNOS(-/-) mice. Six weeks after AC, consequences on morphology function were noninvasively invasively assessed...
Circulating blood-derived vasculogenic cells improve neovascularization of ischemic tissue by a broad repertoire potential therapeutic actions. Whereas initial studies documented that the incorporate and differentiate to cardiovascular cells, other suggested short-time paracrine mechanisms mediate beneficial effects. The question remains what extent physical incorporation is contributing effects cell therapy. By using inducible suicide gene thymidine kinase deplete transplanted we determined...
RATIONALE Chemical cross‐linking in combination with a mass spectrometric analysis of the created cross‐linked products is an area growing interest for deriving low‐resolution structural information proteins and protein complexes. One greatest challenges complexity mixtures, which can be met by charge‐based enrichment peptides after proteolytic digestion using strong cation‐exchange (SCX) chromatography. METHODS SCX chromatography was used N ‐hydroxysuccinimide ester...
To provide a basis for the development of approaches to treat elastin-degrading diseases, aim this study was investigate degradation natural substrate tropoelastin by elastinolytic matrix metalloproteinases MMP-7, MMP-9, and MMP-12 compare cleavage site specificities enzymes using complementary MS techniques molecular modeling. Furthermore, ability three proteases release bioactive peptides studied. Tropoelastin readily degraded all MMPs. Eighty-nine sites in were identified MMP-12, whereas...
In the modern world, crop plants represent a major source of daily consumed foods.Among them, cereals and legumes -i.e. crops accumulating oils, carbohydrates proteins in their seeds -dominate European agriculture, tremendously impacting global protein consumption biodiesel production.Therefore, attract special attention biologists, biochemists, nutritional physiologists food chemists.Seed development germination, as well age-and stress-related changes viability properties, can be addressed...