A5065864748- RNA Research and Splicing
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Chronic Lymphocytic Leukemia Research
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Peptidase Inhibition and Analysis
- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- RNA regulation and disease
- Protein Degradation and Inhibitors
- HER2/EGFR in Cancer Research
- Medical Image Segmentation Techniques
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Image Processing Techniques and Applications
Martin Luther University Halle-Wittenberg
2016-2025
Institute of Molecular Medicine
2012-2025
Stiftung des öffentlichen Rechts an der Martin-Luther-Universität Halle-Wittenberg
2024
Luther University
2011-2023
Chonnam National University Hwasun Hospital
2023
Kemerovo State University
2023
Kyungpook National University
2023
Institute of Molecular Biology
2018
Children's Cancer Institute Australia
2014-2015
German Cancer Research Center
2015
The oncofetal mRNA-binding protein IGF2BP1 and the transcriptional regulator SRF modulate gene expression in cancer. In cancer cells, we demonstrate that promotes of a conserved N6-methyladenosine (m6A)-dependent manner by impairing miRNA-directed decay mRNA. This results enhanced SRF-dependent activity tumor cell growth invasion. At post-transcriptional level, sustains various SRF-target genes. majority these SRF/IGF2BP1-enhanced genes, including PDLIM7 FOXK1, show upregulation with...
The RNA-binding protein IGF2BP1 (IGF-II mRNA binding 1) stabilizes the c-myc RNA by associating with Coding Region instability Determinant (CRD). If and how other proteins cooperate in promoting stabilization of via CRD remained elusive. Here, we identify various that associate an RNA-dependent fashion. Four these (HNRNPU, SYNCRIP, YBX1, DHX9) were essential to ensure CRD. These factors a CRD-dependent manner, co-distribute non-polysomal fractions comprising mRNA, colocalize cytoplasm. A...
Mass spectrometry (MS) can deliver valuable diagnostic data that complement genomic information and allow us to increase our current knowledge of the COVID-19 disease caused by SARS-CoV-2 virus. We developed a simple, MS-based method specifically detect proteins from gargle solution samples patients. The protocol consists an acetone precipitation tryptic digestion contained within solution, followed targeted MS analysis. Our methodology identifies unique peptides originating nucleoprotein....
Background — Vinculin and its isoform metavinculin are protein components of intercalated discs, structures that anchor thin filaments transmit contractile force between cardiac myocytes. We tested the hypothesis heritable dysfunction may contribute to pathogenesis dilated cardiomyopathy (DCM). Methods Results performed mutational analyses metavinculin-specific exon vinculin in 350 unrelated patients with DCM. One missense mutation (Arg975Trp) one 3-bp deletion (Leu954del) were identified....
The vasodilator-stimulated phosphoprotein (VASP) is a major substrate for cyclic nucleotide-dependent kinases in platelets and other cardiovascular cells. It promotes actin nucleation binds to filaments vitro associates with stress fibers VASP-actin interaction salt-sensitive, arguing electrostatic interactions. Hence, phosphorylation may significantly alter the binding properties of VASP. This hypothesis was investigated by analyzing complex formation recombinant murine VASP after...
Chicken embryo fibroblasts (CEFs) localize β-actin mRNA to their lamellae, a process important for the maintenance of cell polarity and motility. The localization requires cis element (zipcode) involves zipcode binding protein 1 (ZBP1), that specifically binds zipcode. Both lamellipodia polarized CEFs. ZBP1 its homologues contain two NH2-terminal RNA recognition motifs (RRMs) four COOH-terminal hnRNP K homology (KH) domains. By using truncations fused GFP in conjunction with situ...
Hepatocarcinogenesis is a stepwise process. It involves several genetic and epigenetic alterations, e.g., loss of tumor suppressor gene expression ( TP53, PTEN, RB ) as well activation oncogenes (c - MYC, MET, BRAF, RAS ). However, the role RNA-binding proteins (RBPs), which regulate oncogene at posttranscriptional level, are not understood in hepatocellular carcinoma (HCC). Here we analyzed RBPs induced human liver cancer, revealing 116 with significant more than 2-fold higher HCC compared...
An essential constituent of the integrated stress response (ISR) is a reversible translational suppression. This mRNA silencing occurs in distinct cytoplasmic foci called granules (SGs), which transiently associate with processing bodies (PBs), typically serving as decay centers. How mRNAs are protected from degradation these structures remains elusive. We identify that Zipcode-binding protein 1 (ZBP1) regulates fate specific nonstressed cells and key regulator turnover during ISR. ZBP1...
Central nervous system myelination requires the synthesis of large amounts myelin basic protein (MBP) at axon–glia contact site. MBP messenger RNA (mRNA) is transported in granules to oligodendroglial processes a translationally silenced state. This process regulated by trans-acting factor heterogeneous nuclear ribonucleoprotein (hnRNP) A2 binding cis-acting response element (A2RE). Release this repression mRNA translation thus essential for myelination. Mice deficient Src family tyrosine...
Small nucleolar and small Cajal body RNAs (snoRNAs scaRNAs) of the H/ACA box C/D type are generated by exonucleolytic shortening longer precursors. Removal last few nucleotides at 3′ end is known to be a distinct step. We report that, in human cells, knock-down poly(A) specific ribonuclease (PARN), previously implicated only mRNA metabolism, causes accumulation oligoadenylated processing intermediates but not RNAs. In agreement with role PARN snoRNA scaRNA processing, enzyme concentrated...
In primary neurons, the oncofetal RNA-binding protein IGF2BP1 (IGF2 mRNA-binding 1) controls spatially restricted β-actin (ACTB) mRNA translation and modulates growth cone guidance. cultured tumor-derived cells, was shown to regulate formation of lamellipodia invadopodia. However, how via which target mRNAs motility cells has remained elusive. this study, we reveal that promotes velocity directionality cell migration by determining cytoplasmic fate two novel mRNAs: MAPK4 PTEN. Inhibition...
Abstract The IGF2 mRNA-binding protein family (IGF2BPs) directs the cytoplasmic fate of various target mRNAs and controls essential cellular functions. three IGF2BP paralogues expressed in mammals comprise two RNA-recognition motifs (RRM) as well four KH domains. How these domains direct paralogue-dependent function remains largely elusive. In this study, we analyze role IGF2BPs by mutational GXXG-GEEG conversion single domain loops context full-length polypeptides. These analyses reveal...
The IGF2 mRNA-binding protein 1 (IGF2BP1) is a non-catalytic post-transcriptional enhancer of tumor growth upregulated and associated with adverse prognosis in solid cancers. However, conserved effector pathway(s) the feasibility targeting IGF2BP1 cancer remained elusive. We reveal that E2F-driven hallmark promotes G1/S cell cycle transition by stabilizing mRNAs encoding positive regulators this checkpoint like E2F1. This IGF2BP1-driven shortening G1 phase relies on 3'UTR-, miRNA-...
During cellular stress, protein synthesis is severely reduced and bulk mRNA recruited to stress granules (SGs). Previously, we showed that the SG-recruited IGF2 mRNA-binding 1 (IGF2BP1) interferes with target degradation during stress. Whether this requires formation of SGs remained elusive. Here, demonstrate sustained inhibition visible concomitant knockdown TIA1, TIAR G3BP1. FRAP photo-conversion studies, however, indicate these proteins only transiently associate SGs. This suggests...
Background Patients with neuroblastoma due to the amplification of a 130-kb genomic DNA region containing MYCN oncogene have poor prognoses.