A5036803864- Peptidase Inhibition and Analysis
- Nitric Oxide and Endothelin Effects
- Enzyme Catalysis and Immobilization
- Click Chemistry and Applications
- Microbial Metabolic Engineering and Bioproduction
- Immune cells in cancer
- Biochemical and Molecular Research
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Research and Splicing
- Quinazolinone synthesis and applications
- Ubiquitin and proteasome pathways
- Synthesis and Biological Evaluation
- Electrochemical sensors and biosensors
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Production and Characterization
- Environmental Toxicology and Ecotoxicology
- Endoplasmic Reticulum Stress and Disease
- Transgenic Plants and Applications
- Studies on Chitinases and Chitosanases
- Toxic Organic Pollutants Impact
- Eicosanoids and Hypertension Pharmacology
- Genetically Modified Organisms Research
UNSW Sydney
2023-2024
Children's Cancer Institute Australia
2024
Cancer Institute of New South Wales
2023
Martin Luther University Halle-Wittenberg
2017-2021
University of Cambridge
2016-2019
Universidade Federal de Santa Catarina
2009-2018
Departamento de Ciência e Tecnologia
2011
Universidade do Extremo Sul Catarinense
2009
Site-selective chemical conjugation of synthetic molecules to proteins expands their functional and therapeutic capacity. Current protein modification methods, based on biochemical technologies, can achieve site selectivity, but these techniques often require extensive sequence engineering or are restricted the N- C-terminus. Here we show computer-assisted design sulfonyl acrylate reagents for a single lysine residue native sequences. This feature designed acrylates, together with innate...
The IGF2 mRNA-binding protein 1 (IGF2BP1) is a non-catalytic post-transcriptional enhancer of tumor growth upregulated and associated with adverse prognosis in solid cancers. However, conserved effector pathway(s) the feasibility targeting IGF2BP1 cancer remained elusive. We reveal that E2F-driven hallmark promotes G1/S cell cycle transition by stabilizing mRNAs encoding positive regulators this checkpoint like E2F1. This IGF2BP1-driven shortening G1 phase relies on 3'UTR-, miRNA-...
NO has important physiological functions in plants, including the adaptative response to pathogen attack. We previously demonstrated that cryptogein, an elicitor of defence reaction produced by oomycete Phytophthora cryptogea, triggers synthesis tobacco. To decipher role tobacco cells elicited present study we performed a proteomic approach order identify proteins undergoing S-nitrosylation. provided evidence cryptogein induced S-nitrosylation several and identified 11 candidates, CDC48...
Profiling techniques have been suggested as a nontargeted approach to detect unintended effects in genetically modified (GM) plants. Seedlings from eight Brazilian maize varieties, four MON810 GM varieties and non-GM isogenic were grown under controlled environmental conditions. Physiological parameters (aerial part weight, main leaf length, chlorophyll total protein contents) compared, some differences observed. Nevertheless, these not constant among all counterparts. Leaf proteomic...
Abstract TNFα is a prominent proinflammatory cytokine and critical mediator for the development of many types cancer such as breast, colon, prostate, cervical, skin, liver, chronic lymphocytic leukemia. Binding to TNFR1 can lead divergent signaling pathways promoting predominantly NF-κB activation but also cell death. We report here that nitric oxide (NO) donor glyceryl trinitrate (GTN) converts TNFα, generated from immune cells or stimulated by chemotherapy, into prodeath in colon mammary...
Fluorophores are indispensable for imaging biological processes. We report the design and synthesis of azide-tagged boronic acid salicylidenehydrazone (BASHY) dyes their use site-selective labelling Annexin V. The V-BASHY conjugate maintained function fluorescence as demonstrated by targeted detection apoptotic cells.
Protein behavior is closely regulated by a plethora of post-translational modifications (PTMs). It therefore desirable to develop approaches design rational PTMs modulate specific protein functions. Here, we report one such method, and illustrate its successful implementation potentiating the anti-aggregation activity molecular chaperone. Molecular chaperones are multifaceted class proteins essential homeostasis, their major functions combat misfolding aggregation, phenomenon linked number...
Falcipain-2 (FP-2) is a key cysteine protease from the malaria parasite Plasmodium falciparum. Many previous studies have identified FP-2 inhibitors; however, none has yet met criteria for an antimalarial drug candidate. In this work, we assayed in-house library of non-peptidic organic compounds, including (E)-chalcones, (E)-N'-benzylidene-benzohydrazides and alkyl-esters gallic acid, assessed activity toward their mechanisms inhibition. The (E)-chalcones 48, 54 66 showed lowest IC50 values...
Lipases are well-known enzymes extensively used in industrial biotransformation processes. Besides, their structural and catalytic characteristics have attracted increasing attention of several industries the last years. In this work, we biophysical molecular modeling tools to assess properties Staphylococcus xylosus lipase (SXL). We studied thermal unfolding protein its zinc-dependent thermotolerance. demonstrated that SXL is able be active stable at moderate temperatures, but feature only...
The field of chemical site-selective modification proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use chemistry probe the role(s) unpaired Cys residues structure function disease relevant proteins. Phosphatases are important players successful infection pathogenic bacteria, which represent a significant health burden, particularly...
Chemical reactions can be performed to covalently modify specific residues in proteins. When applied native enzymes, these chemical modifications greatly expand the available set of building blocks for development biocatalysts. Nucleophilic canonical amino acid sidechains are most readily accessible targets such endeavors. A rich history attempts design enhanced or novel from various protein scaffolds, has paved way a rapidly developing field with growing scientific, industrial, and...
Abstract Cancers are dependent on copper for growth, angiogenesis and metastasis chelators in clinical trials against breast cancer, however the mechanisms of this emerging cancer treatment still largely unexplored. We previously demonstrated that chelation has efficacy mouse models neuroblastoma glioma. Multiomics analyses neuronal cancers suggested RNA-binding proteins were deregulated by chelator treatment. found “RNA Binding” gene set was most significantly ranked molecular function list...
Abstract BACKGROUND Protein post-translational modifications (PTMs) alter the properties of protein through proteolytic cleavage, or addition modifying groups to one more amino acids. Histones are major targets PTMs, including SUMOylation lysine residues and citrullination arginine residues, with both processes able regulate gene transcription. We aimed delineate potential role in epigenetic dysregulation that drives Diffuse Midline Glioma (DMG) tumorigenicity. METHODS AND RESULTS DMG...
Transthyretin-related proteins (TRPs) constitute a family of structurally related to transthyretin (TTR) and are found in large range bacterial, fungal, plant, invertebrate, vertebrate species. However, it was recently recognized that both prokaryotic eukaryotic members this not functionally transthyretins. TRPs fact involved the purine catabolic pathway function as hydroxyisourate hydrolases. An open reading frame encoding protein similar Escherichia coli TRP identified Herbaspirillum...
Site-specific modification approaches have been extensively employed in the development of protein-based technologies. In this field, stability and activity integrity are envisioned features chemically modified proteins. These methods especially used design antibody-drug conjugates (ADCs). Nevertheless, a biochemical feature target protein these reactions is often overlooked, residue specificity. Usually, course developing chemical probes to modify interest (POI), specific amino acids...
Falcipain-2 (FP2) is an important hemoglobinase from the malaria parasite Plasmodium falciparum and a suitable target for development of antimalarial chemotherapy. Many reports have indicated that radical nitrogen species (RNS) including nitric oxide (NO) are inhibitors P. growth promoters recovery symptoms. In this scenario, FP2 emerges as potential RNS, since its inhibition partially hinders growth. We report in vitro did not undergo S-nitrosylation when exposed to NO-donor GSNO. However,...
<p>This file contains: Figure S1: Apoptosis determined by annexin V-FITC/7-AAD. S2: NO prevents TNFa-induced classical NF-kB signaling pathway. S3: Detection of S-nitrosylation cIAP1 with the biotin-switch assay. S4: Example MALDI-TOF-MS spectra S-nitrosylated cIAP1. S5: SW480 stable cell line expressing 6myc-cIAP1-C571/574H. S6: Characterization apoptosis induced GTN/TNFa. S7: Functional analysis ubiquitin E3 ligase activity mutants. S8: TNFa-mediated complex I to promote II. Table...