A5072147297- Enzyme Catalysis and Immobilization
- Blood properties and coagulation
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Transgenic Plants and Applications
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- biodegradable polymer synthesis and properties
- Amino Acid Enzymes and Metabolism
- Enzyme Production and Characterization
- Protein purification and stability
- Protein Structure and Dynamics
- Chemical Synthesis and Analysis
- Analytical Chemistry and Chromatography
- Electrospun Nanofibers in Biomedical Applications
- Photosynthetic Processes and Mechanisms
- Metabolism and Genetic Disorders
- Crystallization and Solubility Studies
- Silk-based biomaterials and applications
- Chemical synthesis and alkaloids
- Chemical Reactions and Isotopes
- Asymmetric Synthesis and Catalysis
- Viral Infectious Diseases and Gene Expression in Insects
- Nanocomposite Films for Food Packaging
- Microbial Metabolic Engineering and Bioproduction
Martin Luther University Halle-Wittenberg
2015-2024
Luther University
2011-2012
University of Stuttgart
1996-2003
N.D. Zelinsky Institute of Organic Chemistry
2002
University of Regensburg
2000
Technische Universität Braunschweig
1992
The female sex in honeybees (Apis spp.) comprises a reproductive queen and sterile worker caste. Nurse bees feed all larvae progressively with caste-specific food jelly until the prepupal stage. Only those that are exclusively fed large amount of royal (RJ) develop into queens [1Snodgrass R.E. Alimentary Canal Its Glands. In Anatomy Physiology Honeybee. McGraw-Hill Book Company, 1925Google Scholar]. RJ is composite secretion two specialized head glands: mandibular glands, which produce...
Abstract Glycosylation of proteins is a key function the biosynthetic‐secretory pathway in endoplasmic reticulum (ER) and Golgi apparatus. Glycosylated play crucial role cell trafficking signaling, cell‐cell adhesion, blood‐group antigenicity, immune response. In addition, glycosylation an important parameter optimization many glycoprotein‐based drugs such as monoclonal antibodies. vitro glycoengineering requires glycosyltransferases well expensive nucleotide sugars. Here, we present...
The synthesis of diastereo- and enantiomerically pure β-aminocyclopropanecarboxylic acids (β-ACCs) is described. Starting from pyrrole, (rac)-4 readily obtained, which was kinetically resolved by enzymatic hydrolysis. Subsequent oxidation (−)-4 deformylation gives rise to the cis-β-ACC derivative (ent)-9, while (+)-10 converted trans-β-ACC 8. Both 8 (ent)-9 their benzyl esters 13 16, being conformationally restricted β-alanine or γ-aminobutyric acid (GABA) derivatives, represent useful...
Gelatin nanoparticles (GNPs) have demonstrated to be beneficial as a biodegradable and biocompatible delivery system. So far, prepared by the two-step desolvation technique were subsequently cross-linked glutaraldehyde guarantee storage stability. Although in vivo vitro toxicological studies not revealed any related undesired effects, an alternative chemical cross-linking could ease future clinical use humans. Therefore, recombinant enzyme microbial transglutaminase was used examine its...
Abstract Transglutaminases (EC 2.3.2.13) form an enzyme family that catalyzes the formation of isopeptide bonds between γ‐carboxamide group glutamine and ε‐amine lysine residues peptides proteins. Other primary amines can be accepted in place lysine. Because their important physiological pathophysiological functions, transglutaminases have been studied for 60 years. However, substrate preferences this class remain largely elusive. In study, we used focused combinatorial libraries 400 to...
Abstract 4‐Hydroxyphenylacetate 3‐hydroxylase (4HPA3H), a flavin‐dependent monooxygenase from E. coli that catalyzes the hydroxylation of monophenols to catechols, was modified by rational redesign convert also more bulky substrates, especially phenolic natural products like phenylpropanoids, flavones or coumarins. Selected amino acid positions in binding pocket 4HPA3H were exchanged with residues homologous protein Pseudomonas aeruginosa , yielding variants improved conversion spacious...
Chronic skin wounds place a high burden on patients and health care systems. The use of angiogenic mitogenic growth factors can facilitate the healing but are quickly inactivated by wound environment if added exogenously. Here, free-standing multilayer films (FSF) fabricated from chitosan alginate as opposing polyelectrolytes in an alternating manner using layer-by-layer technique. One hundred bilayers form about 450 μm thick, detachable film that is subsequently crosslinked either ethyl...
Mussel foot proteins (Mfps) possess unique binding properties to various surfaces due the presence of L-3,4-dihydroxyphenylalanine (DOPA). Mytilus edulis protein-3 (Mefp-3) is one several in byssal adhesive plaque. Its localization at plaque-substrate interface approved that Mefp-3 plays a key role adhesion. Therefore, protein suitable for development innovative bio-based binders. However, recombinant Mfp-3s are mainly purified from inclusion bodies under denaturing conditions. Here, we...
Poly(glycerol adipate)-graft-(poly(ε-caprolactone)-block-poly(ethylene oxide)) (PGA-g-(PCL-b-PEO)) is synthesized by ring opening polymerization of ε-caprolactone initiated the hydroxyl groups PGA. This followed grafting poly(ethylene oxide) monomethylether mPEO-N3 onto PCL blocks using copper-catalyzed azide–alkyne cycloaddition (CuAAC, "click" reaction). All polymers form micelles radii in range 10 nm after dissolving acetone and dialysis against water. Micelles formed PGA-g-(PCL-b-PEO)...
Objectives Gelatin, a degraded collagen, has been widely used as scaffolding material in tissue engineering applications. In this work, we aimed at the development of situ, cross-linking, cytocompatible hydrogels by use transglutaminase cross-linker for potential application regeneration tissues. Methods Hydrogels were prepared from gelatin different concentrations and bloom degree (175 (G175) or 300 (G300) gelatin) cross-linked with various amounts microbial (mTG) 37°C. Mechanical...