A5065016477- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- RNA modifications and cancer
- Enzyme Structure and Function
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Genomics and Chromatin Dynamics
- Gut microbiota and health
- Heat shock proteins research
- Click Chemistry and Applications
- Bacterial Identification and Susceptibility Testing
- Quantum Information and Cryptography
- Quantum Mechanics and Applications
- Cancer, Lipids, and Metabolism
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Genetic Neurodegenerative Diseases
- Toxin Mechanisms and Immunotoxins
- Spectroscopy and Quantum Chemical Studies
- Bacteriophages and microbial interactions
- Escherichia coli research studies
Martin Luther University Halle-Wittenberg
2020-2022
Luther University
2022
University of Michigan
2013-2020
Università di Camerino
2016-2019
Universitätsmedizin Greifswald
2019
Ann Arbor Center for Independent Living
2018
The number of publications in the field chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and probe protein-protein interactions has increased during last years. As technique is now becoming routine vitro vivo applications proteomics structural biology there a pressing need define protocols as well data analysis reporting formats. Such consensus formats should become accepted be shown lead reproducible...
Abstract α‐Synuclein (α‐syn) is an intrinsically disordered protein (IDP) that undergoes liquid‐liquid phase separation (LLPS), fibrillation, and forms insoluble intracellular Lewy bodies in neurons, which are the hallmark of Parkinson's Disease (PD). Neurotoxicity precedes formation aggregates might be related to α‐syn LLPS. The molecular mechanisms underlying early stages LLPS still elusive. To obtain structural insights into upon LLPS, we take advantage cross‐linking/mass spectrometry...
The combination of cross-linking/mass spectrometry (XL-MS) and ion mobility is still underexplored for conducting protein conformational protein–protein interaction studies. We present a method analyzing cross-linking mixtures on timsTOF Pro mass spectrometer that allows separating ions based their gas-phase mobilities. Cross-linking was performed with three urea-based MS-cleavable cross-linkers deliver distinct fragmentation patterns cross-linked species upon collisional activation....
Abstract Cross-linking/mass spectrometry (XL-MS) has come a long way. Originally, XL-MS was used to study relatively small, purified proteins. Meanwhile, it is employed investigate protein-protein interactions on proteome-wide level, giving snapshots of cellular processes. Currently, at the intersection multitude workflows and impact this technique in addressing specific biological questions steadily growing. This article intended give bird’s-eye view current status XL-MS, benefits using...
Abstract Lecithin:cholesterol acyltransferase (LCAT) catalyzes a critical step of reverse cholesterol transport by esterifying in high density lipoprotein (HDL) particles. LCAT is activated apolipoprotein A-I (ApoA-I), which forms double belt around HDL, however the manner engages its lipidic substrates and ApoA-I HDL poorly understood. Here, we used negative stain electron microscopy, crosslinking, hydrogen-deuterium exchange studies to refine molecular details LCAT–HDL complex. Our data...
Cold-stress in Escherichia coli induces de novo synthesis of translation initiation factors IF1, IF2 and IF3 while ribosome assembly slow down. Consequently, the IFs/ribosome stoichiometric ratio increases about 3-fold during first hours cold adaptation. The IF1 increase plays a role regulation at low temperature (cold-shock-induced translational bias) but so far no specific could be attributed to extra copies IF2. In this work, we show that extra-copies made after stress are associated with...
Abstract α‐Synuclein (α‐syn) is an intrinsically disordered protein (IDP) that undergoes liquid‐liquid phase separation (LLPS), fibrillation, and forms insoluble intracellular Lewy bodies in neurons, which are the hallmark of Parkinson's Disease (PD). Neurotoxicity precedes formation aggregates might be related to α‐syn LLPS. The molecular mechanisms underlying early stages LLPS still elusive. To obtain structural insights into upon LLPS, we take advantage cross‐linking/mass spectrometry...
Abstract Protein Y ( PY ) is an E scherichia coli cold‐shock protein which has been proposed to be responsible for the repression of bulk synthesis during cold adaptation. Here, we present in vivo and vitro data clarify role its mechanism action. Deletion yfiA , gene encoding demonstrates that this dispensable adaptation not shutdown at onset stress, although it able partially inhibit translation. In assays reveal extent inhibition changes with different mRNA s related capacity binding 30S...
Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and analysis interactions organellar whole cell extracts. Each exhibits high aqueous solubility, excellent efficiencies, low collision induced dissociation (CID) energy fragmentation stoichiometries reaction, increased charges crosslinked peptide ions, maintenance overall surface charge balance proteins.
The field of quantum sensing aims at improving the detection and estimation classical parameters that are encoded in physical systems by resorting to sources light strategies. same approach can be used improve current measurements performed on biological systems. Here we consider scenario two bacteria (E. coli Salmonella) growing a Luria-Bertani broth monitored spectrophotometers. Their concentration related optical transmissivity via Beer-Lambert-Bouguer's law their growth curves described...
Abstract The number of publications in the field chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and probe protein-protein interactions has largely increased during last years. As technique is now becoming routine vitro vivo applications proteomics structural biology there a pressing need define protocols as well data analysis reporting formats that are generally accepted have shown lead high-quality...
Abstract The combination of cross-linking/mass spectrometry (XL-MS) and ion mobility is still underexplored for conducting protein conformational protein-protein interaction studies. We present a method analyzing cross-linking mixtures on timsTOF Pro mass spectrometer that allows separating ions based their gas phase mobilities. Cross-linking was performed with three urea-based MS-cleavable cross-linkers deliver distinct fragmentation patterns cross-linked species upon collisional...
Abstract α-Synuclein (α-syn) is an intrinsically disordered protein (IDP) that undergoes liquid-liquid phase separation (LLPS), fibrillation, and forms insoluble intracellular Lewy’s bodies in neurons, which are the hallmark of Parkinson’s Disease (PD). Neurotoxicity precedes formation aggregates probably related to LLPS α-syn cell. The molecular mechanisms underlying early stages still elusive. To obtain structural insights into upon LLPS, we take advantage cross-linking/mass spectrometry...