A5053262964- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Phytoplasmas and Hemiptera pathogens
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Parkinson's Disease Mechanisms and Treatments
- Plant Molecular Biology Research
- Cellular transport and secretion
- Blood groups and transfusion
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Plant nutrient uptake and metabolism
- Alzheimer's disease research and treatments
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Blood transfusion and management
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
Université de Bordeaux
2016-2025
Institut Européen de Chimie et Biologie
2024
Chimie et Biologie des Membranes et des Nanoobjects
2024
Institut de Génomique Fonctionnelle
2013-2023
Deutsches Elektronen-Synchrotron DESY
2023
Bordeaux Population Health
2018
Inserm
2007-2018
Institut Polytechnique de Bordeaux
2017-2018
Centre National de la Recherche Scientifique
2002-2017
Proteome Sciences (United Kingdom)
2017
A large body of evidence from the past decade supports existence functional microdomains in membranes animal and yeast cells, which play important roles protein sorting, signal transduction, or infection by pathogens. They are based on dynamic clustering sphingolipids cholesterol ergosterol characterized their insolubility, at low temperature, nonionic detergents. Here we show that similar also exist plant plasma membrane isolated both tobacco leaves BY2 cells. Tobacco lipid rafts were found...
Abstract Plasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made identifying PD proteins, the role played by major membrane constituents, such as lipids, defining specialized domains remains unknown. Through a rigorous isolation of “native” fractions and comparative mass spectrometry-based analysis, we demonstrate that lipids laterally segregated along plasma (PM) at cell-to-cell junction Arabidopsis...
Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, main postsynaptic scaffold at excitatory synapses. Since synthetic recombinant recognize epitopes not directly involved target protein activity, we consider them here as tools to develop PSD-95 probes. After confirming...
A large body of evidence from the past decade supports existence, in membrane animal and yeast cells, functional microdomains that play important roles protein sorting, signal transduction, or infection by pathogens. Recent reports demonstrated presence, plants, detergent-resistant fractions isolated plasma membrane. Analysis lipidic composition this fraction revealed its enrichment sphingolipids sterols depletion phospho- glycerolipids as previously observed for microdomains....
We report here the complete genome sequence of virulent strain JIP02/86 (ATCC 49511) Flavobacterium psychrophilum, a widely distributed pathogen wild and cultured salmonid fish. The consists 2,861,988–base pair (bp) circular chromosome with 2,432 predicted protein-coding genes. Among these proteins, stress response mediators, gliding motility adhesins many putative secreted proteases are probably involved in colonization, invasion destruction host tissues. provides basis for explaining...
Poplar is the first forest tree genome to be decoded. As an initial step comprehensive analysis of poplar proteome, we described reference 2-D-maps for eight tissues/organs plant, and functional characterization some proteins. A total 398 proteins were excised from gels. About 91.2% identified by nanospray LC-MS/MS, based on comparison with 260 000 Populus sp. ESTs. In comparison, reliable PMFs obtained only 51% spots MALDI-TOF-MS, which 43% (83 spots) positively matched gene models...
The characterization of proteins isolated from skin tissue is apparently an essential parameter for understanding grape ripening as this contains the key compounds wine quality. It has been particularly difficult to extract skins analysis by two-dimensional electrophoresis gels and, therefore, a protocol purpose adapted. focus was on evolution proteome profile during maturation. Proteome maps obtained at three stages were compared assess extent which protein distribution differs in ripening....
The number of publications in the field chemical cross-linking combined with mass spectrometry (XL-MS) to derive constraints for protein three-dimensional structure modeling and probe protein-protein interactions has increased during last years. As technique is now becoming routine vitro vivo applications proteomics structural biology there a pressing need define protocols as well data analysis reporting formats. Such consensus formats should become accepted be shown lead reproducible...
Article9 July 2019Open Access Transparent process Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata Marie L Brault Laboratoire de Biogenèse Membranaire, UMR5200, CNRS, Université Bordeaux, Villenave d'Ornon, France Search for more papers by this author Jules D Petit orcid.org/0000-0003-4652-2843 Biophysique Moléculaire aux Interfaces, TERRA Research Centre, GX ABT, Liège, Gembloux, Belgium Françoise Immel orcid.org/0000-0002-2028-902X William J...
Lipid droplets (LDs) are cell compartments specialized for oil storage. Although their role and biogenesis relatively well documented in seeds, little is known about composition, structure function senescing leaves where they also accumulate. Here, we used a label free quantitative mass spectrometry approach to define the LD proteome of aging Arabidopsis leaves. We found that its composition highly different from seed/cotyledon identified 28 proteins including 9 enzymes secondary metabolism...
Podocytes play a key role in diabetic nephropathy pathogenesis, but alteration of their metabolism remains unknown human kidney. By using conditionally differentiating podocyte cell line, we addressed the functional and molecular changes energetics during vitro development or under high glucose conditions. In 5 mM medium, observed stepwise activation oxidative differentiation that was characterized by peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α)-dependent stimulation...
Abstract Aberrant self-assembly and toxicity of wild-type mutant superoxide dismutase 1 (SOD1) has been widely examined in silico, vitro transgenic animal models amyotrophic lateral sclerosis. Detailed examination the protein disease-affected tissues from sclerosis patients, however, remains scarce. We used histological, biochemical analytical techniques to profile alterations SOD1 deposition, subcellular localization, maturation post-translational modification post-mortem spinal cord cases...
Ivory is a highly prized material in many cultures since it can be carved into intricate designs and have polished surface. Due to its popularity, the animals from which ivory sourced are under threat of extinction. Identification species not only important for CITES compliance, also provide information about context work was created. Here, we developed minimally invasive workflow remove minimal amounts precious objects and, using high-resolution mass spectrometry–based proteomics,...
The oil palm fruit mesocarp contains high lipase activity that increases free fatty acids and necessitates post-harvest inactivation by heat treatment of bunches. Even before the causes consequential losses requires costly measures to limit quantities. Here we demonstrate elite low-lipase lines yield with substantially less than standard genotypes, allowing more flexibility for processing extended ripening increased yields. We identify its gene cosegregates low-/high-lipase trait, providing...
Mitochondrial dysfunction in the spinal cord is a hallmark of amyotrophic lateral sclerosis (ALS), but neurometabolic alterations during early stages disease remain unknown. Here, we investigated bioenergetic and proteomic changes ALS mouse motor neurons patients' skin fibroblasts. We first observed that SODG93A mice presymptomatic display coupling efficiency oxidative phosphorylation, along with fragmentation mitochondrial network. The proteome also revealed peculiar metabolic signature...
Mycoplasmas are "minimal" bacteria able to infect humans, wildlife, and a large number of economically important livestock species. Mycoplasma infections include spectrum clinical manifestations ranging from simple fever fulminant inflammatory diseases with high mortality rates. These mostly chronic, suggesting that mycoplasmas have developed means evade the host immune response. Here we present functionally characterize two-protein system mycoides subspecies capri is involved in capture...
Spiroplasmas are helical and motile members of a cell wall-less eubacterial group called Mollicutes. Although all spiroplasmas associated with arthropods, they exhibit great diversity respect to both their modes transmission effects on hosts; ranging from horizontally transmitted pathogens commensals endosymbionts that transovarially (i.e., mother offspring). Here we provide the first genome sequence, along proteomic validation, an endosymbiotic inherited Spiroplasma bacterium, poulsonii...
Stealth α-synuclein amyloids proliferate in preparations of preformed fibrils and form aggressive self-replicating strains.
Significance Redox sensitivity is a common property of temperature-sensitive transient receptor potential (TRP) ion channels. Here we show that oxidation sensitizes and activates rodent TRPV2 orthologs known to have high temperature threshold (>50 °C), but also the heat-insensitive human TRPV2. This oxidation-induced channel gating intact in cell-free membrane patches, cysteine-independent reduced upon replacement methionine residues M528 M607. Blocking reducing agent DTT reduce...
Abstract Dopamine transmission is involved in reward processing and motor control, its impairment plays a central role numerous neurological disorders. Despite strong pathophysiological relevance, the molecular structural organization of dopaminergic synapse remains to be established. Here, we used targeted labelling fluorescence activated sorting purify striatal synaptosomes. We provide proteome synapses with 57 proteins specifically enriched. Beyond canonical markers dopamine...
By stimulating human CD8+ T lymphocytes with autologous dendritic cells infected an adenovirus encoding MAGE-3, we obtained a cytotoxic lymphocyte (CTL) clone that recognized new MAGE-3 antigenic peptide, AELVHFLLL, which is presented by HLA-B40. This peptide also encoded MAGE-12. The CTL MAGE-3–expressing tumor only when they were first treated IFN-γ. Since this treatment known to induce the exchange of three catalytic subunits proteasome form immunoproteasome, result suggested processing...
Wood is one of our most important natural resources. Surprisingly, we know hardly anything about the details process wood formation. The aim this work was to describe main proteins expressed in forming tissue a conifer species (Pinus pinaster Ait.). Using high resolution 2-DE with linear pH gradient ranging from 4 7, total 1039 spots were detected. Out 240 analyzed by MS/MS, 67.9% identified, 16.7% presented no homology databases, and 15.4% corresponded protein mixtures. 57 MALDI-MS, only...
Study of the complexome - all protein complexes cell is essential for a better understanding and more global vision function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, cytosolic membrane Escherichia coli were separated. Then, different partners each complex identified by LC-MS/MS. In this report, 306 separated identified. Among these complexes, 50 heteromultimeric 256 homomultimeric found. 18 previously described validate technology. study, 109 new found,...