A5039533667- Cell Image Analysis Techniques
- RNA Research and Splicing
- Advanced Fluorescence Microscopy Techniques
- Cancer-related molecular mechanisms research
- Lipid Membrane Structure and Behavior
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Quantum Dots Synthesis And Properties
- Diffusion and Search Dynamics
- Prostate Cancer Treatment and Research
- RNA modifications and cancer
- Gold and Silver Nanoparticles Synthesis and Applications
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- ZnO doping and properties
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Radiopharmaceutical Chemistry and Applications
- Retinal Imaging and Analysis
- Luminescence and Fluorescent Materials
- Advanced Electron Microscopy Techniques and Applications
- Mitochondrial Function and Pathology
- Organ Transplantation Techniques and Outcomes
Centre National de la Recherche Scientifique
2011-2024
Université de Bordeaux
2016-2024
Inserm
2016-2024
Imaging Center
2023-2024
Bordeaux Imaging Center
2018-2021
Cancer Research UK
2016-2020
University of Cambridge
2016-2020
Cancer Research UK Cambridge Center
2016-2019
Institut de Pharmacologie et de Biologie Structurale
2011-2013
Université de Toulouse
2011-2013
Abstract Emerging data demonstrate homologous recombination (HR) defects in castration-resistant prostate cancers, rendering these tumours sensitive to PARP inhibition. Here we a direct requirement for the androgen receptor (AR) maintain HR gene expression and activity cancer. We show that PARP-mediated repair pathways are upregulated cancer following androgen-deprivation therapy (ADT). Furthermore, upregulation of is essential survival cells synthetic lethality between ADT inhibition vivo....
Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, main postsynaptic scaffold at excitatory synapses. Since synthetic recombinant recognize epitopes not directly involved target protein activity, we consider them here as tools to develop PSD-95 probes. After confirming...
Loss-of-function (LOF) methods such as RNA interference (RNAi), antisense oligonucleotides or CRISPR-based genome editing provide unparalleled power for studying the biological function of genes interest. However, a major concern is non-specific targeting, which involves depletion transcripts other than those intended. Little work has been performed to characterize off-target effects these common LOF at whole-transcriptome level. Here, we experimentally compared activity RNAi, and CRISPR...
The fluorescence ubiquitination-based cell cycle indicator (FUCCI) is a powerful tool for use in live cells but current FUCCI-based assays have limited throughput terms of image processing and quantification. Here, we developed lentiviral system that rapidly introduced FUCCI transgenes into by using an all-in-one expression cassette, FastFUCCI. approach alleviated the need sequential transduction characterisation, improving labelling efficiency. We coupled to automated imaging workflow...
Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs, we identify numerous lncRNAs involved in key steps cell division such as chromosome segregation, mitotic duration cytokinesis. Here, provide evidence that the chromatin-associated lncRNA, linc00899, leads to robust delay upon its depletion multiple...
We present a systematic study of the influence nanoparticle used as probe in Single Particle Tracking experiments with supported lipid bilayer model system. Quantum Dots, 40 nm and 200 diameter fluorescent latex spheres, gold colloids were attached to headgroup-labeled molecules by means an antibody. The percentage particle's surface covered antibody was also varied. made choice extract diffusion coefficient from first two points mean square displacement particle after demonstrating that...
Liver sinusoidal endothelial cells (LSECs) possess fenestrae, which are key for the exchange between blood and hepatocytes. Alterations in their number or diameter have important implications hepatic function liver diseases. They lost early development of fibrosis through a process called capillarization. In this study, we aimed to demonstrate whether vitro dedifferentiated LSECs that fenestrae able re‐form these structures. Using stimulated emission depletion super‐resolution microscopy...
The water dispersion of luminescent nanocrystals (NCs) synthesized in organic solvent by encapsulation a surfactant bilayer is promising strategy for preserving the optical properties NCs. phase transfer highly monodispersed ZnO NCs using monomer, dimer, trimer and polymer series alkyl ammonium surfactants compared. Transfer yields over 60% could be obtained with oligomers polymer. In contrast, we observed no measurable single chain surfactant. NMR spectroscopy, including DOSY NOESY,...
STED microscopy has been adopted by many research laboratories and imaging core facilities around the world. It offers biologists in principle a much higher spatial resolution than conventional light to reveal morphological molecular details of cells beyond diffraction barrier. However, this advantage is oftentimes hard for users attain presence optical aberrations, which can severely degrade microscope performance. A classic culprit mismatch refractive index between optics biological...
We investigated the "interdigitated double layer" strategy, with objective to transfer water hydrophobic photoluminescent ZnO nanocrystals (Ncs) stabilized by a ligand (octylamine). This strategy relies on formation of layer around Ncs interdigitation an added surfactant within alkyl chains pristine ligand. Various surfactants were evaluated and, surprisingly, could only be achieved limited choice molecular structures. Among them, family glycolic acid ethoxylate ethers yielded transfers up...
Background Information Liver sinusoidal endothelial cells (LSECs) possess fenestrae , open transcellular pores with an average diameter of 100 nm. These allow for the exchange between blood and hepatocytes. Alterations in their number or liver diseases have important implications hepatic microcirculation function. Although decades studies, are still observed into fixed we poor knowledge dynamics. Results Using stimulated emission depletion (STED) super‐resolution microscopy, established a...
ABSTRACT Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, main postsynaptic scaffold at excitatory synapses. Since synthetic recognize epitopes not directly involved target protein activity, we consider them here as tools to develop PSD-95 probes. After confirming their...
ABSTRACT Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture faithfully segregate chromosomes into daughter cells. The role long noncoding RNAs (lncRNAs) in controlling these processes however remains largely unexplored. To identify lncRNAs with mitotic functions, we performed a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs. By investigating major hallmarks cell division such as chromosome segregation,...
ABSTRACT Loss-of-function (LOF) methods, such as RNA interference (RNAi), antisense oligonucleotides or CRISPR-based genome editing, provide unparalleled power for studying the biological function of genes interest. When coupled with transcriptomic analyses, LOF methods allow researchers to dissect networks transcriptional regulation. However, a major concern is nonspecific targeting, which involves depletion transcripts other than those intended. The off-target effects each these common...
Abstract Clinical studies have shown the improved benefit of combining androgen deprivation therapy (ADT) with radiotherapy, indicating a potential interaction between signalling and DNA damage response (DDR) pathways in PCa. We explored underlying basis this by interrogating mechanism regulation DDR men PCa, both untreated treated an inhibitor. show that receptor (AR) regulates promotes foci formation leading to ATM activation Our data showing AR provides explanation for outcome combined...